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Snow Mountain Virus recovery by synthetic human histo-blood group antigens is heavily influenced by matrix effects
Noroviruses are known to bind to histo-blood group antigens (HBGAs) and the specific binding patterns depend on the virus genotype. However, the development of point-of-care diagnostic assays based on this binding has been challenging due to low assay sensitivity. This study utilized a well-defined...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7069939/ https://www.ncbi.nlm.nih.gov/pubmed/32170122 http://dx.doi.org/10.1038/s41598-020-60639-6 |
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author | Kirby, Amy E. Kienast, Yvonne Aldeco, Milagros Steele, Molly Dhawane, Abasaheb N. Liu, Dandan Cui, Xikai Das, Amrita Iyer, Suri Moe, Christine L. |
author_facet | Kirby, Amy E. Kienast, Yvonne Aldeco, Milagros Steele, Molly Dhawane, Abasaheb N. Liu, Dandan Cui, Xikai Das, Amrita Iyer, Suri Moe, Christine L. |
author_sort | Kirby, Amy E. |
collection | PubMed |
description | Noroviruses are known to bind to histo-blood group antigens (HBGAs) and the specific binding patterns depend on the virus genotype. However, the development of point-of-care diagnostic assays based on this binding has been challenging due to low assay sensitivity. This study utilized a well-defined stool collection from a GII.2 Snow Mountain Virus (SMV) human challenge study to investigate virus recovery from stool and emesis samples using HBGA-coated beads. SMV was recovered from H type III-coated beads for 13 stool specimens out of 27 SMV-positive specimens tested. After adjusting for non-specific binding to PEG-coated beads, the mean percent recovery by H type III-coated beads was 308.11% +/− 861.61. Recovery by H type III ligands was subject-specific and weakly correlated with stool consistency. Input virus titer was not correlated with SMV recovery. The results suggest that the generally low virus recovery we observed may be due to bead saturation or hindrance by existing glycans in the matrix that precluded the virus from being captured by the synthetic glycans. These results indicate a strong role for subject-specific and matrix effects in HBGA binding by SMV. Further investigation of the nature of this interference is needed to facilitate development of high sensitivity diagnostic assays. |
format | Online Article Text |
id | pubmed-7069939 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-70699392020-03-22 Snow Mountain Virus recovery by synthetic human histo-blood group antigens is heavily influenced by matrix effects Kirby, Amy E. Kienast, Yvonne Aldeco, Milagros Steele, Molly Dhawane, Abasaheb N. Liu, Dandan Cui, Xikai Das, Amrita Iyer, Suri Moe, Christine L. Sci Rep Article Noroviruses are known to bind to histo-blood group antigens (HBGAs) and the specific binding patterns depend on the virus genotype. However, the development of point-of-care diagnostic assays based on this binding has been challenging due to low assay sensitivity. This study utilized a well-defined stool collection from a GII.2 Snow Mountain Virus (SMV) human challenge study to investigate virus recovery from stool and emesis samples using HBGA-coated beads. SMV was recovered from H type III-coated beads for 13 stool specimens out of 27 SMV-positive specimens tested. After adjusting for non-specific binding to PEG-coated beads, the mean percent recovery by H type III-coated beads was 308.11% +/− 861.61. Recovery by H type III ligands was subject-specific and weakly correlated with stool consistency. Input virus titer was not correlated with SMV recovery. The results suggest that the generally low virus recovery we observed may be due to bead saturation or hindrance by existing glycans in the matrix that precluded the virus from being captured by the synthetic glycans. These results indicate a strong role for subject-specific and matrix effects in HBGA binding by SMV. Further investigation of the nature of this interference is needed to facilitate development of high sensitivity diagnostic assays. Nature Publishing Group UK 2020-03-13 /pmc/articles/PMC7069939/ /pubmed/32170122 http://dx.doi.org/10.1038/s41598-020-60639-6 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Kirby, Amy E. Kienast, Yvonne Aldeco, Milagros Steele, Molly Dhawane, Abasaheb N. Liu, Dandan Cui, Xikai Das, Amrita Iyer, Suri Moe, Christine L. Snow Mountain Virus recovery by synthetic human histo-blood group antigens is heavily influenced by matrix effects |
title | Snow Mountain Virus recovery by synthetic human histo-blood group antigens is heavily influenced by matrix effects |
title_full | Snow Mountain Virus recovery by synthetic human histo-blood group antigens is heavily influenced by matrix effects |
title_fullStr | Snow Mountain Virus recovery by synthetic human histo-blood group antigens is heavily influenced by matrix effects |
title_full_unstemmed | Snow Mountain Virus recovery by synthetic human histo-blood group antigens is heavily influenced by matrix effects |
title_short | Snow Mountain Virus recovery by synthetic human histo-blood group antigens is heavily influenced by matrix effects |
title_sort | snow mountain virus recovery by synthetic human histo-blood group antigens is heavily influenced by matrix effects |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7069939/ https://www.ncbi.nlm.nih.gov/pubmed/32170122 http://dx.doi.org/10.1038/s41598-020-60639-6 |
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