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Cell-bound IgE and plasma IgE as a combined clinical diagnostic indicator for allergic patients

Allergic responses are mainly caused by IgE, which is often located on the cell surface. The current diagnostic method detects both allergen-specific IgE and total IgE levels, but a number of allergic patients have a normal serum IgE level, which is a poor clinical correlate for allergy. Here, we de...

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Autores principales: Qiu, Chuanghua, Zhong, Lihong, Huang, Chunxiu, Long, Jia, Ye, Xuejun, Wu, Jingbo, Dai, Wenjie, Lv, Wei, Xie, Chongwei, Zhang, Junfang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7069990/
https://www.ncbi.nlm.nih.gov/pubmed/32170187
http://dx.doi.org/10.1038/s41598-020-61455-8
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author Qiu, Chuanghua
Zhong, Lihong
Huang, Chunxiu
Long, Jia
Ye, Xuejun
Wu, Jingbo
Dai, Wenjie
Lv, Wei
Xie, Chongwei
Zhang, Junfang
author_facet Qiu, Chuanghua
Zhong, Lihong
Huang, Chunxiu
Long, Jia
Ye, Xuejun
Wu, Jingbo
Dai, Wenjie
Lv, Wei
Xie, Chongwei
Zhang, Junfang
author_sort Qiu, Chuanghua
collection PubMed
description Allergic responses are mainly caused by IgE, which is often located on the cell surface. The current diagnostic method detects both allergen-specific IgE and total IgE levels, but a number of allergic patients have a normal serum IgE level, which is a poor clinical correlate for allergy. Here, we developed a simple method to detect the level of cell-bound IgE by dissociating it from blood cells with lactic acid. Dissociated cell-bound IgE and plasma IgE levels were detected using the same ELISA kit at the same time. We established two clinical cohorts: an allergic patient group and a healthy participant group. In general, cell-bound IgE correlated well with plasma IgE; however, some patients exhibited high cell-bound IgE levels but low plasma IgE levels. We recommended 350 ng/mL peripheral blood total IgE (cell-bound IgE + plasma IgE) as the cut-off value for allergy diagnosis. Using this indicator, 90.32% of our allergic patients were correctly diagnosed. The peripheral blood total IgE level is a promising clinical diagnostic indicator in allergic patients and will provide more guidance for allergy diagnosis and therapeutic evaluation.
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spelling pubmed-70699902020-03-22 Cell-bound IgE and plasma IgE as a combined clinical diagnostic indicator for allergic patients Qiu, Chuanghua Zhong, Lihong Huang, Chunxiu Long, Jia Ye, Xuejun Wu, Jingbo Dai, Wenjie Lv, Wei Xie, Chongwei Zhang, Junfang Sci Rep Article Allergic responses are mainly caused by IgE, which is often located on the cell surface. The current diagnostic method detects both allergen-specific IgE and total IgE levels, but a number of allergic patients have a normal serum IgE level, which is a poor clinical correlate for allergy. Here, we developed a simple method to detect the level of cell-bound IgE by dissociating it from blood cells with lactic acid. Dissociated cell-bound IgE and plasma IgE levels were detected using the same ELISA kit at the same time. We established two clinical cohorts: an allergic patient group and a healthy participant group. In general, cell-bound IgE correlated well with plasma IgE; however, some patients exhibited high cell-bound IgE levels but low plasma IgE levels. We recommended 350 ng/mL peripheral blood total IgE (cell-bound IgE + plasma IgE) as the cut-off value for allergy diagnosis. Using this indicator, 90.32% of our allergic patients were correctly diagnosed. The peripheral blood total IgE level is a promising clinical diagnostic indicator in allergic patients and will provide more guidance for allergy diagnosis and therapeutic evaluation. Nature Publishing Group UK 2020-03-13 /pmc/articles/PMC7069990/ /pubmed/32170187 http://dx.doi.org/10.1038/s41598-020-61455-8 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Qiu, Chuanghua
Zhong, Lihong
Huang, Chunxiu
Long, Jia
Ye, Xuejun
Wu, Jingbo
Dai, Wenjie
Lv, Wei
Xie, Chongwei
Zhang, Junfang
Cell-bound IgE and plasma IgE as a combined clinical diagnostic indicator for allergic patients
title Cell-bound IgE and plasma IgE as a combined clinical diagnostic indicator for allergic patients
title_full Cell-bound IgE and plasma IgE as a combined clinical diagnostic indicator for allergic patients
title_fullStr Cell-bound IgE and plasma IgE as a combined clinical diagnostic indicator for allergic patients
title_full_unstemmed Cell-bound IgE and plasma IgE as a combined clinical diagnostic indicator for allergic patients
title_short Cell-bound IgE and plasma IgE as a combined clinical diagnostic indicator for allergic patients
title_sort cell-bound ige and plasma ige as a combined clinical diagnostic indicator for allergic patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7069990/
https://www.ncbi.nlm.nih.gov/pubmed/32170187
http://dx.doi.org/10.1038/s41598-020-61455-8
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