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Sjögren syndrome/scleroderma autoantigen 1 is a direct Tankyrase binding partner in cancer cells
Sjögren syndrome/scleroderma autoantigen 1 (SSSCA1) was first described as an auto-antigen over-expressed in Sjögren’s syndrome and in scleroderma patients. SSSCA1 has been linked to mitosis and centromere association and as a potential marker candidate in diverse solid cancers. Here we characterize...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7070046/ https://www.ncbi.nlm.nih.gov/pubmed/32170109 http://dx.doi.org/10.1038/s42003-020-0851-2 |
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author | Perdreau-Dahl, Harmonie Progida, Cinzia Barfeld, Stefan J. Guldsten, Hanne Thiede, Bernd Arntzen, Magnus Bakke, Oddmund Mills, Ian G. Krauss, Stefan Morth, J. Preben |
author_facet | Perdreau-Dahl, Harmonie Progida, Cinzia Barfeld, Stefan J. Guldsten, Hanne Thiede, Bernd Arntzen, Magnus Bakke, Oddmund Mills, Ian G. Krauss, Stefan Morth, J. Preben |
author_sort | Perdreau-Dahl, Harmonie |
collection | PubMed |
description | Sjögren syndrome/scleroderma autoantigen 1 (SSSCA1) was first described as an auto-antigen over-expressed in Sjögren’s syndrome and in scleroderma patients. SSSCA1 has been linked to mitosis and centromere association and as a potential marker candidate in diverse solid cancers. Here we characterize SSSCA1 for the first time, to our knowledge, at the molecular, structural and subcellular level. We have determined the crystal structure of a zinc finger fold, a zinc ribbon domain type 2 (ZNRD2), at 2.3 Å resolution. We show that the C-terminal domain serves a dual function as it both behaves as the interaction site to Tankyrase 1 (TNKS1) and as a nuclear export signal. We identify TNKS1 as a direct binding partner of SSSCA1, map the binding site to TNKS1 ankyrin repeat cluster 2 (ARC2) and thus define a new binding sequence. We experimentally verify and map a new nuclear export signal sequence in SSSCA1. |
format | Online Article Text |
id | pubmed-7070046 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-70700462020-03-19 Sjögren syndrome/scleroderma autoantigen 1 is a direct Tankyrase binding partner in cancer cells Perdreau-Dahl, Harmonie Progida, Cinzia Barfeld, Stefan J. Guldsten, Hanne Thiede, Bernd Arntzen, Magnus Bakke, Oddmund Mills, Ian G. Krauss, Stefan Morth, J. Preben Commun Biol Article Sjögren syndrome/scleroderma autoantigen 1 (SSSCA1) was first described as an auto-antigen over-expressed in Sjögren’s syndrome and in scleroderma patients. SSSCA1 has been linked to mitosis and centromere association and as a potential marker candidate in diverse solid cancers. Here we characterize SSSCA1 for the first time, to our knowledge, at the molecular, structural and subcellular level. We have determined the crystal structure of a zinc finger fold, a zinc ribbon domain type 2 (ZNRD2), at 2.3 Å resolution. We show that the C-terminal domain serves a dual function as it both behaves as the interaction site to Tankyrase 1 (TNKS1) and as a nuclear export signal. We identify TNKS1 as a direct binding partner of SSSCA1, map the binding site to TNKS1 ankyrin repeat cluster 2 (ARC2) and thus define a new binding sequence. We experimentally verify and map a new nuclear export signal sequence in SSSCA1. Nature Publishing Group UK 2020-03-13 /pmc/articles/PMC7070046/ /pubmed/32170109 http://dx.doi.org/10.1038/s42003-020-0851-2 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Perdreau-Dahl, Harmonie Progida, Cinzia Barfeld, Stefan J. Guldsten, Hanne Thiede, Bernd Arntzen, Magnus Bakke, Oddmund Mills, Ian G. Krauss, Stefan Morth, J. Preben Sjögren syndrome/scleroderma autoantigen 1 is a direct Tankyrase binding partner in cancer cells |
title | Sjögren syndrome/scleroderma autoantigen 1 is a direct Tankyrase binding partner in cancer cells |
title_full | Sjögren syndrome/scleroderma autoantigen 1 is a direct Tankyrase binding partner in cancer cells |
title_fullStr | Sjögren syndrome/scleroderma autoantigen 1 is a direct Tankyrase binding partner in cancer cells |
title_full_unstemmed | Sjögren syndrome/scleroderma autoantigen 1 is a direct Tankyrase binding partner in cancer cells |
title_short | Sjögren syndrome/scleroderma autoantigen 1 is a direct Tankyrase binding partner in cancer cells |
title_sort | sjögren syndrome/scleroderma autoantigen 1 is a direct tankyrase binding partner in cancer cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7070046/ https://www.ncbi.nlm.nih.gov/pubmed/32170109 http://dx.doi.org/10.1038/s42003-020-0851-2 |
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