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Sjögren syndrome/scleroderma autoantigen 1 is a direct Tankyrase binding partner in cancer cells

Sjögren syndrome/scleroderma autoantigen 1 (SSSCA1) was first described as an auto-antigen over-expressed in Sjögren’s syndrome and in scleroderma patients. SSSCA1 has been linked to mitosis and centromere association and as a potential marker candidate in diverse solid cancers. Here we characterize...

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Autores principales: Perdreau-Dahl, Harmonie, Progida, Cinzia, Barfeld, Stefan J., Guldsten, Hanne, Thiede, Bernd, Arntzen, Magnus, Bakke, Oddmund, Mills, Ian G., Krauss, Stefan, Morth, J. Preben
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7070046/
https://www.ncbi.nlm.nih.gov/pubmed/32170109
http://dx.doi.org/10.1038/s42003-020-0851-2
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author Perdreau-Dahl, Harmonie
Progida, Cinzia
Barfeld, Stefan J.
Guldsten, Hanne
Thiede, Bernd
Arntzen, Magnus
Bakke, Oddmund
Mills, Ian G.
Krauss, Stefan
Morth, J. Preben
author_facet Perdreau-Dahl, Harmonie
Progida, Cinzia
Barfeld, Stefan J.
Guldsten, Hanne
Thiede, Bernd
Arntzen, Magnus
Bakke, Oddmund
Mills, Ian G.
Krauss, Stefan
Morth, J. Preben
author_sort Perdreau-Dahl, Harmonie
collection PubMed
description Sjögren syndrome/scleroderma autoantigen 1 (SSSCA1) was first described as an auto-antigen over-expressed in Sjögren’s syndrome and in scleroderma patients. SSSCA1 has been linked to mitosis and centromere association and as a potential marker candidate in diverse solid cancers. Here we characterize SSSCA1 for the first time, to our knowledge, at the molecular, structural and subcellular level. We have determined the crystal structure of a zinc finger fold, a zinc ribbon domain type 2 (ZNRD2), at 2.3 Å resolution. We show that the C-terminal domain serves a dual function as it both behaves as the interaction site to Tankyrase 1 (TNKS1) and as a nuclear export signal. We identify TNKS1 as a direct binding partner of SSSCA1, map the binding site to TNKS1 ankyrin repeat cluster 2 (ARC2) and thus define a new binding sequence. We experimentally verify and map a new nuclear export signal sequence in SSSCA1.
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spelling pubmed-70700462020-03-19 Sjögren syndrome/scleroderma autoantigen 1 is a direct Tankyrase binding partner in cancer cells Perdreau-Dahl, Harmonie Progida, Cinzia Barfeld, Stefan J. Guldsten, Hanne Thiede, Bernd Arntzen, Magnus Bakke, Oddmund Mills, Ian G. Krauss, Stefan Morth, J. Preben Commun Biol Article Sjögren syndrome/scleroderma autoantigen 1 (SSSCA1) was first described as an auto-antigen over-expressed in Sjögren’s syndrome and in scleroderma patients. SSSCA1 has been linked to mitosis and centromere association and as a potential marker candidate in diverse solid cancers. Here we characterize SSSCA1 for the first time, to our knowledge, at the molecular, structural and subcellular level. We have determined the crystal structure of a zinc finger fold, a zinc ribbon domain type 2 (ZNRD2), at 2.3 Å resolution. We show that the C-terminal domain serves a dual function as it both behaves as the interaction site to Tankyrase 1 (TNKS1) and as a nuclear export signal. We identify TNKS1 as a direct binding partner of SSSCA1, map the binding site to TNKS1 ankyrin repeat cluster 2 (ARC2) and thus define a new binding sequence. We experimentally verify and map a new nuclear export signal sequence in SSSCA1. Nature Publishing Group UK 2020-03-13 /pmc/articles/PMC7070046/ /pubmed/32170109 http://dx.doi.org/10.1038/s42003-020-0851-2 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Perdreau-Dahl, Harmonie
Progida, Cinzia
Barfeld, Stefan J.
Guldsten, Hanne
Thiede, Bernd
Arntzen, Magnus
Bakke, Oddmund
Mills, Ian G.
Krauss, Stefan
Morth, J. Preben
Sjögren syndrome/scleroderma autoantigen 1 is a direct Tankyrase binding partner in cancer cells
title Sjögren syndrome/scleroderma autoantigen 1 is a direct Tankyrase binding partner in cancer cells
title_full Sjögren syndrome/scleroderma autoantigen 1 is a direct Tankyrase binding partner in cancer cells
title_fullStr Sjögren syndrome/scleroderma autoantigen 1 is a direct Tankyrase binding partner in cancer cells
title_full_unstemmed Sjögren syndrome/scleroderma autoantigen 1 is a direct Tankyrase binding partner in cancer cells
title_short Sjögren syndrome/scleroderma autoantigen 1 is a direct Tankyrase binding partner in cancer cells
title_sort sjögren syndrome/scleroderma autoantigen 1 is a direct tankyrase binding partner in cancer cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7070046/
https://www.ncbi.nlm.nih.gov/pubmed/32170109
http://dx.doi.org/10.1038/s42003-020-0851-2
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