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Dynamics of bacterial population growth in biofilms resemble spatial and structural aspects of urbanization
Biofilms develop from bacteria bound on surfaces that grow into structured communities (microcolonies). Although surface topography is known to affect bacterial colonization, how multiple individual settlers develop into microcolonies simultaneously remains underexplored. Here, we use multiscale pop...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7070081/ https://www.ncbi.nlm.nih.gov/pubmed/32170131 http://dx.doi.org/10.1038/s41467-020-15165-4 |
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author | Paula, Amauri J. Hwang, Geelsu Koo, Hyun |
author_facet | Paula, Amauri J. Hwang, Geelsu Koo, Hyun |
author_sort | Paula, Amauri J. |
collection | PubMed |
description | Biofilms develop from bacteria bound on surfaces that grow into structured communities (microcolonies). Although surface topography is known to affect bacterial colonization, how multiple individual settlers develop into microcolonies simultaneously remains underexplored. Here, we use multiscale population-growth and 3D-morphometric analyses to assess the spatiotemporal development of hundreds of bacterial colonizers towards submillimeter-scale microcolony communities. Using an oral bacterium (Streptococcus mutans), we find that microbial cells settle on the surface randomly under sucrose-rich conditions, regardless of surface topography. However, only a subset of colonizers display clustering behavior and growth following a power law. These active colonizers expand three-dimensionally by amalgamating neighboring bacteria into densely populated microcolonies. Clustering and microcolony assembly are dependent on exopolysaccharides, while population growth dynamics and spatial structure are affected by cooperative or antagonistic microbes. Our work suggests that biofilm assembly resembles certain spatial-structural features of urbanization, where population growth and expansion can be influenced by type of settlers, neighboring cells, and further community merging and scaffolding occurring at various scales. |
format | Online Article Text |
id | pubmed-7070081 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-70700812020-03-18 Dynamics of bacterial population growth in biofilms resemble spatial and structural aspects of urbanization Paula, Amauri J. Hwang, Geelsu Koo, Hyun Nat Commun Article Biofilms develop from bacteria bound on surfaces that grow into structured communities (microcolonies). Although surface topography is known to affect bacterial colonization, how multiple individual settlers develop into microcolonies simultaneously remains underexplored. Here, we use multiscale population-growth and 3D-morphometric analyses to assess the spatiotemporal development of hundreds of bacterial colonizers towards submillimeter-scale microcolony communities. Using an oral bacterium (Streptococcus mutans), we find that microbial cells settle on the surface randomly under sucrose-rich conditions, regardless of surface topography. However, only a subset of colonizers display clustering behavior and growth following a power law. These active colonizers expand three-dimensionally by amalgamating neighboring bacteria into densely populated microcolonies. Clustering and microcolony assembly are dependent on exopolysaccharides, while population growth dynamics and spatial structure are affected by cooperative or antagonistic microbes. Our work suggests that biofilm assembly resembles certain spatial-structural features of urbanization, where population growth and expansion can be influenced by type of settlers, neighboring cells, and further community merging and scaffolding occurring at various scales. Nature Publishing Group UK 2020-03-13 /pmc/articles/PMC7070081/ /pubmed/32170131 http://dx.doi.org/10.1038/s41467-020-15165-4 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Paula, Amauri J. Hwang, Geelsu Koo, Hyun Dynamics of bacterial population growth in biofilms resemble spatial and structural aspects of urbanization |
title | Dynamics of bacterial population growth in biofilms resemble spatial and structural aspects of urbanization |
title_full | Dynamics of bacterial population growth in biofilms resemble spatial and structural aspects of urbanization |
title_fullStr | Dynamics of bacterial population growth in biofilms resemble spatial and structural aspects of urbanization |
title_full_unstemmed | Dynamics of bacterial population growth in biofilms resemble spatial and structural aspects of urbanization |
title_short | Dynamics of bacterial population growth in biofilms resemble spatial and structural aspects of urbanization |
title_sort | dynamics of bacterial population growth in biofilms resemble spatial and structural aspects of urbanization |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7070081/ https://www.ncbi.nlm.nih.gov/pubmed/32170131 http://dx.doi.org/10.1038/s41467-020-15165-4 |
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