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Modulatory Effect of Chelidonium majus Extract and Its Alkaloids on LPS-Stimulated Cytokine Secretion in Human Neutrophils

Due to certain differences in terms of molecular structure, isoquinoline alkaloids from Chelidonium majus engage in various biological activities. Apart from their well-documented antimicrobial potential, some phenanthridine and protoberberine derivatives as well as C. majus extract present with ant...

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Autores principales: Zielińska, Sylwia, Czerwińska, Monika Ewa, Dziągwa-Becker, Magdalena, Dryś, Andrzej, Kucharski, Mariusz, Jezierska-Domaradzka, Anna, Płachno, Bartosz J., Matkowski, Adam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7070267/
https://www.ncbi.nlm.nih.gov/pubmed/32075082
http://dx.doi.org/10.3390/molecules25040842
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author Zielińska, Sylwia
Czerwińska, Monika Ewa
Dziągwa-Becker, Magdalena
Dryś, Andrzej
Kucharski, Mariusz
Jezierska-Domaradzka, Anna
Płachno, Bartosz J.
Matkowski, Adam
author_facet Zielińska, Sylwia
Czerwińska, Monika Ewa
Dziągwa-Becker, Magdalena
Dryś, Andrzej
Kucharski, Mariusz
Jezierska-Domaradzka, Anna
Płachno, Bartosz J.
Matkowski, Adam
author_sort Zielińska, Sylwia
collection PubMed
description Due to certain differences in terms of molecular structure, isoquinoline alkaloids from Chelidonium majus engage in various biological activities. Apart from their well-documented antimicrobial potential, some phenanthridine and protoberberine derivatives as well as C. majus extract present with anti-inflammatory and cytotoxic effects. In this study, the LC–MS/MS method was used to determine alkaloids, phenolic acids, carboxylic acids, and hydroxybenzoic acids. We investigated five individually tested alkaloids (coptisine, berberine, chelidonine, chelerythrine, and sanguinarine) as well as C. majus root extract for their effect on the secretion of IL-1β, IL-8, and TNF-α in human polymorphonuclear leukocytes (neutrophils). Berberine, chelidonine, and chelerythrine significantly decreased the secretion of TNF-α in a concentration-dependent manner. Sanguinarine was found to be the most potent inhibitor of IL-1β secretion. However, the overproduction of IL-8 and TNF-α and a high cytotoxicity for these compounds were observed. Coptisine was highly cytotoxic and slightly decreased the secretion of the studied cytokines. The extract (1.25–12.5 μg/mL) increased cytokine secretion in a concentration-dependent manner, but an increase in cytotoxicity was also noted. The alkaloids were active at very low concentrations (0.625–2.5 μM), but their potential cytotoxic effects, except for chelidonine and chelerythrine, should not be ignored.
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spelling pubmed-70702672020-03-19 Modulatory Effect of Chelidonium majus Extract and Its Alkaloids on LPS-Stimulated Cytokine Secretion in Human Neutrophils Zielińska, Sylwia Czerwińska, Monika Ewa Dziągwa-Becker, Magdalena Dryś, Andrzej Kucharski, Mariusz Jezierska-Domaradzka, Anna Płachno, Bartosz J. Matkowski, Adam Molecules Article Due to certain differences in terms of molecular structure, isoquinoline alkaloids from Chelidonium majus engage in various biological activities. Apart from their well-documented antimicrobial potential, some phenanthridine and protoberberine derivatives as well as C. majus extract present with anti-inflammatory and cytotoxic effects. In this study, the LC–MS/MS method was used to determine alkaloids, phenolic acids, carboxylic acids, and hydroxybenzoic acids. We investigated five individually tested alkaloids (coptisine, berberine, chelidonine, chelerythrine, and sanguinarine) as well as C. majus root extract for their effect on the secretion of IL-1β, IL-8, and TNF-α in human polymorphonuclear leukocytes (neutrophils). Berberine, chelidonine, and chelerythrine significantly decreased the secretion of TNF-α in a concentration-dependent manner. Sanguinarine was found to be the most potent inhibitor of IL-1β secretion. However, the overproduction of IL-8 and TNF-α and a high cytotoxicity for these compounds were observed. Coptisine was highly cytotoxic and slightly decreased the secretion of the studied cytokines. The extract (1.25–12.5 μg/mL) increased cytokine secretion in a concentration-dependent manner, but an increase in cytotoxicity was also noted. The alkaloids were active at very low concentrations (0.625–2.5 μM), but their potential cytotoxic effects, except for chelidonine and chelerythrine, should not be ignored. MDPI 2020-02-14 /pmc/articles/PMC7070267/ /pubmed/32075082 http://dx.doi.org/10.3390/molecules25040842 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zielińska, Sylwia
Czerwińska, Monika Ewa
Dziągwa-Becker, Magdalena
Dryś, Andrzej
Kucharski, Mariusz
Jezierska-Domaradzka, Anna
Płachno, Bartosz J.
Matkowski, Adam
Modulatory Effect of Chelidonium majus Extract and Its Alkaloids on LPS-Stimulated Cytokine Secretion in Human Neutrophils
title Modulatory Effect of Chelidonium majus Extract and Its Alkaloids on LPS-Stimulated Cytokine Secretion in Human Neutrophils
title_full Modulatory Effect of Chelidonium majus Extract and Its Alkaloids on LPS-Stimulated Cytokine Secretion in Human Neutrophils
title_fullStr Modulatory Effect of Chelidonium majus Extract and Its Alkaloids on LPS-Stimulated Cytokine Secretion in Human Neutrophils
title_full_unstemmed Modulatory Effect of Chelidonium majus Extract and Its Alkaloids on LPS-Stimulated Cytokine Secretion in Human Neutrophils
title_short Modulatory Effect of Chelidonium majus Extract and Its Alkaloids on LPS-Stimulated Cytokine Secretion in Human Neutrophils
title_sort modulatory effect of chelidonium majus extract and its alkaloids on lps-stimulated cytokine secretion in human neutrophils
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7070267/
https://www.ncbi.nlm.nih.gov/pubmed/32075082
http://dx.doi.org/10.3390/molecules25040842
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