Cargando…

Discovery of Antifungal and Biofilm Preventative Compounds from Mycelial Cultures of a Unique North American Hericium sp. Fungus

Edible mushrooms are an important source of nutraceuticals and for the discovery of bioactive metabolites as pharmaceuticals. In this work, the OSMAC (One Strain, Many Active Compounds) approach was used to isolate two new compounds (1 and 2) along with seven known compounds (3–9) from a mycelial cu...

Descripción completa

Detalles Bibliográficos
Autores principales: Song, Xun, Gaascht, François, Schmidt-Dannert, Claudia, Salomon, Christine E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7070493/
https://www.ncbi.nlm.nih.gov/pubmed/32093422
http://dx.doi.org/10.3390/molecules25040963
_version_ 1783505988063592448
author Song, Xun
Gaascht, François
Schmidt-Dannert, Claudia
Salomon, Christine E.
author_facet Song, Xun
Gaascht, François
Schmidt-Dannert, Claudia
Salomon, Christine E.
author_sort Song, Xun
collection PubMed
description Edible mushrooms are an important source of nutraceuticals and for the discovery of bioactive metabolites as pharmaceuticals. In this work, the OSMAC (One Strain, Many Active Compounds) approach was used to isolate two new compounds (1 and 2) along with seven known compounds (3–9) from a mycelial culture of a unique North American edible mushroom Hericium sp. The fruiting body was collected in Marine on St. Croix, Minnesota (USA), and mycelial cultures were grown on four different solid and liquid media. Extracts from the mycelial cultures were screened for antimicrobial activity and only the extract from the Cheerios substrate culture exhibited antifungal activity. Bioassay guided fractionation and HPLC analysis were used to isolate nine pure compounds and the structures of the known compounds were established by analysis of the NMR and mass spectrometry data and comparison to published reports. Compound 1 is a new erinacerin alkaloid and 2 is an aldehyde derivative of 4-hydroxy chroman. Four chlorinated orcinol derivatives (3–6), a pyran (7), erinaceolactone (8), and erinacine (9) were identified. Compound 4 showed antifungal activity against C. albicans and C. neoformans (MIC = 31.3–62.5 μg/mL, respectively). Compound 4 also inhibited biofilm formation of C. albicans and C. neoformans at 7.8 μg/mL. These results suggest that mycelial cultures of edible fungi may provide useful, bioactive compounds.
format Online
Article
Text
id pubmed-7070493
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-70704932020-03-19 Discovery of Antifungal and Biofilm Preventative Compounds from Mycelial Cultures of a Unique North American Hericium sp. Fungus Song, Xun Gaascht, François Schmidt-Dannert, Claudia Salomon, Christine E. Molecules Article Edible mushrooms are an important source of nutraceuticals and for the discovery of bioactive metabolites as pharmaceuticals. In this work, the OSMAC (One Strain, Many Active Compounds) approach was used to isolate two new compounds (1 and 2) along with seven known compounds (3–9) from a mycelial culture of a unique North American edible mushroom Hericium sp. The fruiting body was collected in Marine on St. Croix, Minnesota (USA), and mycelial cultures were grown on four different solid and liquid media. Extracts from the mycelial cultures were screened for antimicrobial activity and only the extract from the Cheerios substrate culture exhibited antifungal activity. Bioassay guided fractionation and HPLC analysis were used to isolate nine pure compounds and the structures of the known compounds were established by analysis of the NMR and mass spectrometry data and comparison to published reports. Compound 1 is a new erinacerin alkaloid and 2 is an aldehyde derivative of 4-hydroxy chroman. Four chlorinated orcinol derivatives (3–6), a pyran (7), erinaceolactone (8), and erinacine (9) were identified. Compound 4 showed antifungal activity against C. albicans and C. neoformans (MIC = 31.3–62.5 μg/mL, respectively). Compound 4 also inhibited biofilm formation of C. albicans and C. neoformans at 7.8 μg/mL. These results suggest that mycelial cultures of edible fungi may provide useful, bioactive compounds. MDPI 2020-02-20 /pmc/articles/PMC7070493/ /pubmed/32093422 http://dx.doi.org/10.3390/molecules25040963 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Song, Xun
Gaascht, François
Schmidt-Dannert, Claudia
Salomon, Christine E.
Discovery of Antifungal and Biofilm Preventative Compounds from Mycelial Cultures of a Unique North American Hericium sp. Fungus
title Discovery of Antifungal and Biofilm Preventative Compounds from Mycelial Cultures of a Unique North American Hericium sp. Fungus
title_full Discovery of Antifungal and Biofilm Preventative Compounds from Mycelial Cultures of a Unique North American Hericium sp. Fungus
title_fullStr Discovery of Antifungal and Biofilm Preventative Compounds from Mycelial Cultures of a Unique North American Hericium sp. Fungus
title_full_unstemmed Discovery of Antifungal and Biofilm Preventative Compounds from Mycelial Cultures of a Unique North American Hericium sp. Fungus
title_short Discovery of Antifungal and Biofilm Preventative Compounds from Mycelial Cultures of a Unique North American Hericium sp. Fungus
title_sort discovery of antifungal and biofilm preventative compounds from mycelial cultures of a unique north american hericium sp. fungus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7070493/
https://www.ncbi.nlm.nih.gov/pubmed/32093422
http://dx.doi.org/10.3390/molecules25040963
work_keys_str_mv AT songxun discoveryofantifungalandbiofilmpreventativecompoundsfrommycelialculturesofauniquenorthamericanhericiumspfungus
AT gaaschtfrancois discoveryofantifungalandbiofilmpreventativecompoundsfrommycelialculturesofauniquenorthamericanhericiumspfungus
AT schmidtdannertclaudia discoveryofantifungalandbiofilmpreventativecompoundsfrommycelialculturesofauniquenorthamericanhericiumspfungus
AT salomonchristinee discoveryofantifungalandbiofilmpreventativecompoundsfrommycelialculturesofauniquenorthamericanhericiumspfungus