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Identification of Important Proteins and Pathways Affecting Feed Efficiency in DLY Pigs by iTRAQ-Based Proteomic Analysis

SIMPLE SUMMARY: Feed efficiency is one of the most valuable economic traits in the pig industry. The small intestine is the site where most of the nutrients are absorbed from ingested food. Here, we studied the relationship between small intestinal proteomics and feed efficiency in Duroc × (Landrace...

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Autores principales: Wu, Jie, Wang, Xingwang, Ding, Rongrong, Quan, Jianping, Ye, Yong, Gu, Ting, Xu, Zheng, Zheng, Enqin, Cai, Gengyuan, Wu, Zhenfang, Yang, Ming, Yang, Jie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7070517/
https://www.ncbi.nlm.nih.gov/pubmed/31978958
http://dx.doi.org/10.3390/ani10020189
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author Wu, Jie
Wang, Xingwang
Ding, Rongrong
Quan, Jianping
Ye, Yong
Gu, Ting
Xu, Zheng
Zheng, Enqin
Cai, Gengyuan
Wu, Zhenfang
Yang, Ming
Yang, Jie
author_facet Wu, Jie
Wang, Xingwang
Ding, Rongrong
Quan, Jianping
Ye, Yong
Gu, Ting
Xu, Zheng
Zheng, Enqin
Cai, Gengyuan
Wu, Zhenfang
Yang, Ming
Yang, Jie
author_sort Wu, Jie
collection PubMed
description SIMPLE SUMMARY: Feed efficiency is one of the most valuable economic traits in the pig industry. The small intestine is the site where most of the nutrients are absorbed from ingested food. Here, we studied the relationship between small intestinal proteomics and feed efficiency in Duroc × (Landrace × Yorkshire) pigs, which is the most popular commercial pig in the Chinese pork market. Exploring the molecular mechanisms of feed efficiency will create great value for the pig industry. Our research provided a reference for further understanding of the key proteins that affect small intestinal microvilli formation and the important pathways related to feed efficiency in pigs. ABSTRACT: Feed efficiency is an economically important trait controlled by multiple genes in pigs. The small intestine is the main organ of digestion and nutrient absorption. To explore the biological processes by which small intestine proteomics affects feed efficiency (FE), we investigated the small intestinal tissue proteomes of high-FE and low-FE pigs by the isobaric tag for relative and absolute quantification (iTRAQ) method. In this study, a total of 225 Duroc × (Landrace × Yorkshire) (DLY) commercial pigs were ranked according to feed efficiency, which ranged from 30 kg to 100 kg, and six pigs with extreme phenotypes were selected, three in each of the high and low groups. A total of 1219 differentially expressed proteins (DEPs) were identified between the high-FE and low-FE groups (fold change ≥1.2 or ≤0.84; p ≤ 0.05), of which 785 were upregulated, and 484 were downregulated. Enrichment analysis indicated that the DEPs were mainly enriched in actin filament formation, microvilli formation, and small intestinal movement pathways. Protein functional analysis and protein interaction networks indicated that RHOA, HCLS1, EZR, CDC42, and RAC1 were important proteins that regulate FE in pigs. This study provided new insights into the important pathways and proteins involved in feed efficiency in pigs.
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spelling pubmed-70705172020-03-19 Identification of Important Proteins and Pathways Affecting Feed Efficiency in DLY Pigs by iTRAQ-Based Proteomic Analysis Wu, Jie Wang, Xingwang Ding, Rongrong Quan, Jianping Ye, Yong Gu, Ting Xu, Zheng Zheng, Enqin Cai, Gengyuan Wu, Zhenfang Yang, Ming Yang, Jie Animals (Basel) Article SIMPLE SUMMARY: Feed efficiency is one of the most valuable economic traits in the pig industry. The small intestine is the site where most of the nutrients are absorbed from ingested food. Here, we studied the relationship between small intestinal proteomics and feed efficiency in Duroc × (Landrace × Yorkshire) pigs, which is the most popular commercial pig in the Chinese pork market. Exploring the molecular mechanisms of feed efficiency will create great value for the pig industry. Our research provided a reference for further understanding of the key proteins that affect small intestinal microvilli formation and the important pathways related to feed efficiency in pigs. ABSTRACT: Feed efficiency is an economically important trait controlled by multiple genes in pigs. The small intestine is the main organ of digestion and nutrient absorption. To explore the biological processes by which small intestine proteomics affects feed efficiency (FE), we investigated the small intestinal tissue proteomes of high-FE and low-FE pigs by the isobaric tag for relative and absolute quantification (iTRAQ) method. In this study, a total of 225 Duroc × (Landrace × Yorkshire) (DLY) commercial pigs were ranked according to feed efficiency, which ranged from 30 kg to 100 kg, and six pigs with extreme phenotypes were selected, three in each of the high and low groups. A total of 1219 differentially expressed proteins (DEPs) were identified between the high-FE and low-FE groups (fold change ≥1.2 or ≤0.84; p ≤ 0.05), of which 785 were upregulated, and 484 were downregulated. Enrichment analysis indicated that the DEPs were mainly enriched in actin filament formation, microvilli formation, and small intestinal movement pathways. Protein functional analysis and protein interaction networks indicated that RHOA, HCLS1, EZR, CDC42, and RAC1 were important proteins that regulate FE in pigs. This study provided new insights into the important pathways and proteins involved in feed efficiency in pigs. MDPI 2020-01-22 /pmc/articles/PMC7070517/ /pubmed/31978958 http://dx.doi.org/10.3390/ani10020189 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wu, Jie
Wang, Xingwang
Ding, Rongrong
Quan, Jianping
Ye, Yong
Gu, Ting
Xu, Zheng
Zheng, Enqin
Cai, Gengyuan
Wu, Zhenfang
Yang, Ming
Yang, Jie
Identification of Important Proteins and Pathways Affecting Feed Efficiency in DLY Pigs by iTRAQ-Based Proteomic Analysis
title Identification of Important Proteins and Pathways Affecting Feed Efficiency in DLY Pigs by iTRAQ-Based Proteomic Analysis
title_full Identification of Important Proteins and Pathways Affecting Feed Efficiency in DLY Pigs by iTRAQ-Based Proteomic Analysis
title_fullStr Identification of Important Proteins and Pathways Affecting Feed Efficiency in DLY Pigs by iTRAQ-Based Proteomic Analysis
title_full_unstemmed Identification of Important Proteins and Pathways Affecting Feed Efficiency in DLY Pigs by iTRAQ-Based Proteomic Analysis
title_short Identification of Important Proteins and Pathways Affecting Feed Efficiency in DLY Pigs by iTRAQ-Based Proteomic Analysis
title_sort identification of important proteins and pathways affecting feed efficiency in dly pigs by itraq-based proteomic analysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7070517/
https://www.ncbi.nlm.nih.gov/pubmed/31978958
http://dx.doi.org/10.3390/ani10020189
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