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Identification of New Peptides from Fermented Milk Showing Antioxidant Properties: Mechanism of Action
Due to their beneficial properties, fermented foods are considered important constituents of the human diet. They also contain bioactive peptides, health-promoting compounds studied for a wide range of effects. In this work, several antioxidant peptides extracted from fermented milk proteins were in...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7070694/ https://www.ncbi.nlm.nih.gov/pubmed/32013158 http://dx.doi.org/10.3390/antiox9020117 |
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author | Tonolo, Federica Fiorese, Federico Moretto, Laura Folda, Alessandra Scalcon, Valeria Grinzato, Alessandro Ferro, Stefania Arrigoni, Giorgio Bindoli, Alberto Feller, Emiliano Bellamio, Marco Marin, Oriano Rigobello, Maria Pia |
author_facet | Tonolo, Federica Fiorese, Federico Moretto, Laura Folda, Alessandra Scalcon, Valeria Grinzato, Alessandro Ferro, Stefania Arrigoni, Giorgio Bindoli, Alberto Feller, Emiliano Bellamio, Marco Marin, Oriano Rigobello, Maria Pia |
author_sort | Tonolo, Federica |
collection | PubMed |
description | Due to their beneficial properties, fermented foods are considered important constituents of the human diet. They also contain bioactive peptides, health-promoting compounds studied for a wide range of effects. In this work, several antioxidant peptides extracted from fermented milk proteins were investigated. First, enriched peptide fractions were purified and analysed for their antioxidant capacity in vitro and in a cellular model. Subsequently, from the most active fractions, 23 peptides were identified by mass spectrometry MS/MS), synthesized and tested. Peptides N-15-M, E-11-F, Q-14-R and A-17-E were selected for their antioxidant effects on Caco-2 cells both in the protection against oxidative stress and inhibition of ROS production. To define their action mechanism, the activation of the Kelch-like ECH-associated protein 1/nuclear factor erythroid 2-related factor 2(Keap1/Nrf2) pathway was studied evaluating the translocation of Nrf2 from cytosol to nucleus. In cells treated with N-15-M, Q-14-R and A-17-E, a higher amount of Nrf2 was found in the nucleus with respect to the control. In addition, the three active peptides, through the activation of Keap1/Nrf2 pathway, led to overexpression and increased activity of antioxidant enzymes. Molecular docking analysis confirmed the potential ability of N-15-M, Q-14-R and A-17-E to bind Keap1, showing their destabilizing effect on Keap1/Nrf2 interaction. |
format | Online Article Text |
id | pubmed-7070694 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-70706942020-03-19 Identification of New Peptides from Fermented Milk Showing Antioxidant Properties: Mechanism of Action Tonolo, Federica Fiorese, Federico Moretto, Laura Folda, Alessandra Scalcon, Valeria Grinzato, Alessandro Ferro, Stefania Arrigoni, Giorgio Bindoli, Alberto Feller, Emiliano Bellamio, Marco Marin, Oriano Rigobello, Maria Pia Antioxidants (Basel) Article Due to their beneficial properties, fermented foods are considered important constituents of the human diet. They also contain bioactive peptides, health-promoting compounds studied for a wide range of effects. In this work, several antioxidant peptides extracted from fermented milk proteins were investigated. First, enriched peptide fractions were purified and analysed for their antioxidant capacity in vitro and in a cellular model. Subsequently, from the most active fractions, 23 peptides were identified by mass spectrometry MS/MS), synthesized and tested. Peptides N-15-M, E-11-F, Q-14-R and A-17-E were selected for their antioxidant effects on Caco-2 cells both in the protection against oxidative stress and inhibition of ROS production. To define their action mechanism, the activation of the Kelch-like ECH-associated protein 1/nuclear factor erythroid 2-related factor 2(Keap1/Nrf2) pathway was studied evaluating the translocation of Nrf2 from cytosol to nucleus. In cells treated with N-15-M, Q-14-R and A-17-E, a higher amount of Nrf2 was found in the nucleus with respect to the control. In addition, the three active peptides, through the activation of Keap1/Nrf2 pathway, led to overexpression and increased activity of antioxidant enzymes. Molecular docking analysis confirmed the potential ability of N-15-M, Q-14-R and A-17-E to bind Keap1, showing their destabilizing effect on Keap1/Nrf2 interaction. MDPI 2020-01-29 /pmc/articles/PMC7070694/ /pubmed/32013158 http://dx.doi.org/10.3390/antiox9020117 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Tonolo, Federica Fiorese, Federico Moretto, Laura Folda, Alessandra Scalcon, Valeria Grinzato, Alessandro Ferro, Stefania Arrigoni, Giorgio Bindoli, Alberto Feller, Emiliano Bellamio, Marco Marin, Oriano Rigobello, Maria Pia Identification of New Peptides from Fermented Milk Showing Antioxidant Properties: Mechanism of Action |
title | Identification of New Peptides from Fermented Milk Showing Antioxidant Properties: Mechanism of Action |
title_full | Identification of New Peptides from Fermented Milk Showing Antioxidant Properties: Mechanism of Action |
title_fullStr | Identification of New Peptides from Fermented Milk Showing Antioxidant Properties: Mechanism of Action |
title_full_unstemmed | Identification of New Peptides from Fermented Milk Showing Antioxidant Properties: Mechanism of Action |
title_short | Identification of New Peptides from Fermented Milk Showing Antioxidant Properties: Mechanism of Action |
title_sort | identification of new peptides from fermented milk showing antioxidant properties: mechanism of action |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7070694/ https://www.ncbi.nlm.nih.gov/pubmed/32013158 http://dx.doi.org/10.3390/antiox9020117 |
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