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Comparison of Single, Fixed-Time Artificial Insemination in Gilts Using Two Different Protocols to Synchronize Ovulation

SIMPLE SUMMARY: It is necessary to have a consistent supply of service-ready gilts available to incorporate into each batch of breeding sows. Techniques to manipulate the timing of estrus and possibly the timing of ovulation in gilts are helpful in order to achieve this goal. This study investigated...

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Autores principales: Rodrigues, Lima, Amezcua, Rocio, Cassar, Glen, O’Sullivan, Terri L, Friendship, Robert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7070714/
https://www.ncbi.nlm.nih.gov/pubmed/32075043
http://dx.doi.org/10.3390/ani10020306
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author Rodrigues, Lima
Amezcua, Rocio
Cassar, Glen
O’Sullivan, Terri L
Friendship, Robert
author_facet Rodrigues, Lima
Amezcua, Rocio
Cassar, Glen
O’Sullivan, Terri L
Friendship, Robert
author_sort Rodrigues, Lima
collection PubMed
description SIMPLE SUMMARY: It is necessary to have a consistent supply of service-ready gilts available to incorporate into each batch of breeding sows. Techniques to manipulate the timing of estrus and possibly the timing of ovulation in gilts are helpful in order to achieve this goal. This study investigated two different techniques for inducing ovulation to allow fixed-time artificial insemination (FTAI) protocols in gilts and compared results with gilts bred when observed in standing heat following cessation of daily altrenogest treatment. Pubertal gilts (n = 180) were assigned to one of three treatment groups. Group 1 gilts (LUT, n = 62) were induced to ovulate utilizing intramuscular injections of equine chorionic gonadotropin followed by porcine luteinizing hormone, and bred using a single FTAI. Group 2 gilts (TRI, n = 61) were induced to ovulate by intravaginal deposition of triptorelin acetate and were bred by a single FTAI. Group 3 gilts (CON, n = 57) were observed for estrus and bred twice (24 h apart) using artificial insemination (AI). LUT and TRI gilts completed farrowing in a smaller window of time compared to CON gilts; however, they also tended to have poorer reproductive performance. LUT and TRI piglets were 80 g and 64 g, respectively, heavier at weaning than CON piglets. Results indicate that FTAI might be useful as a means of minimizing the range in lactation length in a farrowing batch. However, modifications of the protocols may be required to ensure optimum farrowing rates and litter size. ABSTRACT: In order to efficiently have a consistent supply of service-ready gilts available to incorporate into each batch of breeding sows, it is necessary to manipulate the timing of estrus and possibly the timing of ovulation of gilts. Estrus can be synchronized by the withdrawal of altrenogest after at least 14 days of treatment. It is possible that protocols developed to induce ovulation, and therefore allow fixed-time artificial insemination (FTAI), can improve the predictability of gilt breeding. This study investigated the effect of two FTAI protocols in gilts on reproductive performance and timing of farrowing and piglet weaning weight compared to gilts bred based on signs of estrus after cessation of altrenogest. Puberty was induced in gilts, followed by treatment with altrenogest. Following altrenogest withdrawal, 180 gilts were assigned to one of three treatment groups. Group 1 gilts (LUT, n = 62) were treated with 600 IU equine chorionic gonadotropin 24 h after altrenogest withdrawal and 5 mg porcine luteinizing hormone (pLH) 80 h later, followed by a single FTAI 36 h after pLH. Group 2 gilts (TRI, n= 61) received 2 mL of a gonadotropin-releasing hormone agonist, triptorelin acetate, intravaginally 6 d after altrenogest withdrawal and were bred by a single FTAI 24 h later. Group 3 gilts (CON, n = 57) were observed for estrus and bred twice by AI, 24 h apart. LUT and TRI gilts farrowed closer together (2.4 ± 1.6 and 2.9 ± 1.2 d(days), respectively) compared to CON gilts (4.5 ± 3.3 d). Piglets in LUT were 80 g (p < 0.001) heavier and piglets in TRI were 64 g (p < 0.05) heavier at weaning than CON piglets, when controlling for birth weight. Results indicate that FTAI might be useful as a means of minimizing the time from the first to the last gilt farrowing in a breeding batch of gilts. However, modifications of the protocols may be required to ensure optimum farrowing rates and litter size.
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spelling pubmed-70707142020-03-19 Comparison of Single, Fixed-Time Artificial Insemination in Gilts Using Two Different Protocols to Synchronize Ovulation Rodrigues, Lima Amezcua, Rocio Cassar, Glen O’Sullivan, Terri L Friendship, Robert Animals (Basel) Article SIMPLE SUMMARY: It is necessary to have a consistent supply of service-ready gilts available to incorporate into each batch of breeding sows. Techniques to manipulate the timing of estrus and possibly the timing of ovulation in gilts are helpful in order to achieve this goal. This study investigated two different techniques for inducing ovulation to allow fixed-time artificial insemination (FTAI) protocols in gilts and compared results with gilts bred when observed in standing heat following cessation of daily altrenogest treatment. Pubertal gilts (n = 180) were assigned to one of three treatment groups. Group 1 gilts (LUT, n = 62) were induced to ovulate utilizing intramuscular injections of equine chorionic gonadotropin followed by porcine luteinizing hormone, and bred using a single FTAI. Group 2 gilts (TRI, n = 61) were induced to ovulate by intravaginal deposition of triptorelin acetate and were bred by a single FTAI. Group 3 gilts (CON, n = 57) were observed for estrus and bred twice (24 h apart) using artificial insemination (AI). LUT and TRI gilts completed farrowing in a smaller window of time compared to CON gilts; however, they also tended to have poorer reproductive performance. LUT and TRI piglets were 80 g and 64 g, respectively, heavier at weaning than CON piglets. Results indicate that FTAI might be useful as a means of minimizing the range in lactation length in a farrowing batch. However, modifications of the protocols may be required to ensure optimum farrowing rates and litter size. ABSTRACT: In order to efficiently have a consistent supply of service-ready gilts available to incorporate into each batch of breeding sows, it is necessary to manipulate the timing of estrus and possibly the timing of ovulation of gilts. Estrus can be synchronized by the withdrawal of altrenogest after at least 14 days of treatment. It is possible that protocols developed to induce ovulation, and therefore allow fixed-time artificial insemination (FTAI), can improve the predictability of gilt breeding. This study investigated the effect of two FTAI protocols in gilts on reproductive performance and timing of farrowing and piglet weaning weight compared to gilts bred based on signs of estrus after cessation of altrenogest. Puberty was induced in gilts, followed by treatment with altrenogest. Following altrenogest withdrawal, 180 gilts were assigned to one of three treatment groups. Group 1 gilts (LUT, n = 62) were treated with 600 IU equine chorionic gonadotropin 24 h after altrenogest withdrawal and 5 mg porcine luteinizing hormone (pLH) 80 h later, followed by a single FTAI 36 h after pLH. Group 2 gilts (TRI, n= 61) received 2 mL of a gonadotropin-releasing hormone agonist, triptorelin acetate, intravaginally 6 d after altrenogest withdrawal and were bred by a single FTAI 24 h later. Group 3 gilts (CON, n = 57) were observed for estrus and bred twice by AI, 24 h apart. LUT and TRI gilts farrowed closer together (2.4 ± 1.6 and 2.9 ± 1.2 d(days), respectively) compared to CON gilts (4.5 ± 3.3 d). Piglets in LUT were 80 g (p < 0.001) heavier and piglets in TRI were 64 g (p < 0.05) heavier at weaning than CON piglets, when controlling for birth weight. Results indicate that FTAI might be useful as a means of minimizing the time from the first to the last gilt farrowing in a breeding batch of gilts. However, modifications of the protocols may be required to ensure optimum farrowing rates and litter size. MDPI 2020-02-14 /pmc/articles/PMC7070714/ /pubmed/32075043 http://dx.doi.org/10.3390/ani10020306 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rodrigues, Lima
Amezcua, Rocio
Cassar, Glen
O’Sullivan, Terri L
Friendship, Robert
Comparison of Single, Fixed-Time Artificial Insemination in Gilts Using Two Different Protocols to Synchronize Ovulation
title Comparison of Single, Fixed-Time Artificial Insemination in Gilts Using Two Different Protocols to Synchronize Ovulation
title_full Comparison of Single, Fixed-Time Artificial Insemination in Gilts Using Two Different Protocols to Synchronize Ovulation
title_fullStr Comparison of Single, Fixed-Time Artificial Insemination in Gilts Using Two Different Protocols to Synchronize Ovulation
title_full_unstemmed Comparison of Single, Fixed-Time Artificial Insemination in Gilts Using Two Different Protocols to Synchronize Ovulation
title_short Comparison of Single, Fixed-Time Artificial Insemination in Gilts Using Two Different Protocols to Synchronize Ovulation
title_sort comparison of single, fixed-time artificial insemination in gilts using two different protocols to synchronize ovulation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7070714/
https://www.ncbi.nlm.nih.gov/pubmed/32075043
http://dx.doi.org/10.3390/ani10020306
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