Cargando…

Aerobic Training Down-Regulates Pentraxin 3 and Pentraxin 3/Toll-Like Receptor 4 Ratio, Irrespective of Oxidative Stress Response, in Elderly Subjects

Reactive oxygen and nitrogen species-mediated cellular aging has been linked to diseases such as atherothrombosis and cancer. Although pentraxin 3 (PTX3) is associated with aging-related diseases via TLR4-dependent anti-inflammatory effects, its relationship with oxidative stress in aging remains to...

Descripción completa

Detalles Bibliográficos
Autores principales: Estébanez, Brisamar, Rodriguez, Alexandra L., Visavadiya, Nishant P., Whitehurst, Michael, Cuevas, María J., González-Gallego, Javier, Huang, Chun-Jung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7070734/
https://www.ncbi.nlm.nih.gov/pubmed/32012711
http://dx.doi.org/10.3390/antiox9020110
_version_ 1783506043481882624
author Estébanez, Brisamar
Rodriguez, Alexandra L.
Visavadiya, Nishant P.
Whitehurst, Michael
Cuevas, María J.
González-Gallego, Javier
Huang, Chun-Jung
author_facet Estébanez, Brisamar
Rodriguez, Alexandra L.
Visavadiya, Nishant P.
Whitehurst, Michael
Cuevas, María J.
González-Gallego, Javier
Huang, Chun-Jung
author_sort Estébanez, Brisamar
collection PubMed
description Reactive oxygen and nitrogen species-mediated cellular aging has been linked to diseases such as atherothrombosis and cancer. Although pentraxin 3 (PTX3) is associated with aging-related diseases via TLR4-dependent anti-inflammatory effects, its relationship with oxidative stress in aging remains to be elucidated. Exercise is proposed as the key intervention for health maintenance in the elderly. This study aimed to examine the association of PTX3 levels with changes in oxidative stress in both plasma and peripheral blood mononuclear cells (PBMCs), following aerobic training in elderly adults. Nine trained and five controls participated in an eight-week aerobic training protocol. Enzyme-linked immunosorbent assay (ELISA) and Western blot analyses were used to determine PTX3, toll-like receptor 4 (TLR4), and levels of oxidative stress biomarkers [3-nitrotyrosine (3NT), 4-hydroxynonenal (4-HNE), glutathione (GSH), protein carbonyl (PC), reactive oxygen/ nitrogen species (ROS/RNS), and trolox equivalent antioxidant capacity (TEAC)] in plasma and/or PBMCs. Results showed a down-regulation of PTX3 expression in PBMCs following aerobic training, along with decreased PTX3/TLR4 ratios. Oxidative stress responses in PBMCs remained unchanged with the exercise protocol. Comparable levels of plasma PTX3 and oxidative stress biomarkers were observed in trained vs. control groups. No correlation was found between PTX3 and any oxidative stress biomarkers following training. These findings demonstrated the down-regulation of PTX3 and PTX3/TLR4 ratio, irrespective of oxidative stress response, in elderly adults following eight weeks of aerobic training.
format Online
Article
Text
id pubmed-7070734
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-70707342020-03-19 Aerobic Training Down-Regulates Pentraxin 3 and Pentraxin 3/Toll-Like Receptor 4 Ratio, Irrespective of Oxidative Stress Response, in Elderly Subjects Estébanez, Brisamar Rodriguez, Alexandra L. Visavadiya, Nishant P. Whitehurst, Michael Cuevas, María J. González-Gallego, Javier Huang, Chun-Jung Antioxidants (Basel) Article Reactive oxygen and nitrogen species-mediated cellular aging has been linked to diseases such as atherothrombosis and cancer. Although pentraxin 3 (PTX3) is associated with aging-related diseases via TLR4-dependent anti-inflammatory effects, its relationship with oxidative stress in aging remains to be elucidated. Exercise is proposed as the key intervention for health maintenance in the elderly. This study aimed to examine the association of PTX3 levels with changes in oxidative stress in both plasma and peripheral blood mononuclear cells (PBMCs), following aerobic training in elderly adults. Nine trained and five controls participated in an eight-week aerobic training protocol. Enzyme-linked immunosorbent assay (ELISA) and Western blot analyses were used to determine PTX3, toll-like receptor 4 (TLR4), and levels of oxidative stress biomarkers [3-nitrotyrosine (3NT), 4-hydroxynonenal (4-HNE), glutathione (GSH), protein carbonyl (PC), reactive oxygen/ nitrogen species (ROS/RNS), and trolox equivalent antioxidant capacity (TEAC)] in plasma and/or PBMCs. Results showed a down-regulation of PTX3 expression in PBMCs following aerobic training, along with decreased PTX3/TLR4 ratios. Oxidative stress responses in PBMCs remained unchanged with the exercise protocol. Comparable levels of plasma PTX3 and oxidative stress biomarkers were observed in trained vs. control groups. No correlation was found between PTX3 and any oxidative stress biomarkers following training. These findings demonstrated the down-regulation of PTX3 and PTX3/TLR4 ratio, irrespective of oxidative stress response, in elderly adults following eight weeks of aerobic training. MDPI 2020-01-27 /pmc/articles/PMC7070734/ /pubmed/32012711 http://dx.doi.org/10.3390/antiox9020110 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Estébanez, Brisamar
Rodriguez, Alexandra L.
Visavadiya, Nishant P.
Whitehurst, Michael
Cuevas, María J.
González-Gallego, Javier
Huang, Chun-Jung
Aerobic Training Down-Regulates Pentraxin 3 and Pentraxin 3/Toll-Like Receptor 4 Ratio, Irrespective of Oxidative Stress Response, in Elderly Subjects
title Aerobic Training Down-Regulates Pentraxin 3 and Pentraxin 3/Toll-Like Receptor 4 Ratio, Irrespective of Oxidative Stress Response, in Elderly Subjects
title_full Aerobic Training Down-Regulates Pentraxin 3 and Pentraxin 3/Toll-Like Receptor 4 Ratio, Irrespective of Oxidative Stress Response, in Elderly Subjects
title_fullStr Aerobic Training Down-Regulates Pentraxin 3 and Pentraxin 3/Toll-Like Receptor 4 Ratio, Irrespective of Oxidative Stress Response, in Elderly Subjects
title_full_unstemmed Aerobic Training Down-Regulates Pentraxin 3 and Pentraxin 3/Toll-Like Receptor 4 Ratio, Irrespective of Oxidative Stress Response, in Elderly Subjects
title_short Aerobic Training Down-Regulates Pentraxin 3 and Pentraxin 3/Toll-Like Receptor 4 Ratio, Irrespective of Oxidative Stress Response, in Elderly Subjects
title_sort aerobic training down-regulates pentraxin 3 and pentraxin 3/toll-like receptor 4 ratio, irrespective of oxidative stress response, in elderly subjects
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7070734/
https://www.ncbi.nlm.nih.gov/pubmed/32012711
http://dx.doi.org/10.3390/antiox9020110
work_keys_str_mv AT estebanezbrisamar aerobictrainingdownregulatespentraxin3andpentraxin3tolllikereceptor4ratioirrespectiveofoxidativestressresponseinelderlysubjects
AT rodriguezalexandral aerobictrainingdownregulatespentraxin3andpentraxin3tolllikereceptor4ratioirrespectiveofoxidativestressresponseinelderlysubjects
AT visavadiyanishantp aerobictrainingdownregulatespentraxin3andpentraxin3tolllikereceptor4ratioirrespectiveofoxidativestressresponseinelderlysubjects
AT whitehurstmichael aerobictrainingdownregulatespentraxin3andpentraxin3tolllikereceptor4ratioirrespectiveofoxidativestressresponseinelderlysubjects
AT cuevasmariaj aerobictrainingdownregulatespentraxin3andpentraxin3tolllikereceptor4ratioirrespectiveofoxidativestressresponseinelderlysubjects
AT gonzalezgallegojavier aerobictrainingdownregulatespentraxin3andpentraxin3tolllikereceptor4ratioirrespectiveofoxidativestressresponseinelderlysubjects
AT huangchunjung aerobictrainingdownregulatespentraxin3andpentraxin3tolllikereceptor4ratioirrespectiveofoxidativestressresponseinelderlysubjects