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Extraction and Determination of Vitamin K(1) in Foods by Ultrasound-Assisted Extraction, SPE, and LC-MS/MS
Vitamin K(1) is one of the important hydrophobic vitamins in fat-containing foods. Traditionally, lipase is employed in the determination of vitamin K(1) to remove the lipids, which makes the detection complex, time-consuming, and insensitive. In this study, the determination of vitamin K(1) in fat-...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7070738/ https://www.ncbi.nlm.nih.gov/pubmed/32075057 http://dx.doi.org/10.3390/molecules25040839 |
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author | Xu, Yueqing Zhang, Liangxiao Yang, Ruinan Yu, Xu Yu, Li Ma, Fei Li, Hui Wang, Xiupin Li, Peiwu |
author_facet | Xu, Yueqing Zhang, Liangxiao Yang, Ruinan Yu, Xu Yu, Li Ma, Fei Li, Hui Wang, Xiupin Li, Peiwu |
author_sort | Xu, Yueqing |
collection | PubMed |
description | Vitamin K(1) is one of the important hydrophobic vitamins in fat-containing foods. Traditionally, lipase is employed in the determination of vitamin K(1) to remove the lipids, which makes the detection complex, time-consuming, and insensitive. In this study, the determination of vitamin K(1) in fat-containing foods was developed based on ultrasound-assisted extraction (UAE), solid-phase extraction (SPE) combined with liquid chromatography–tandem mass spectrometry (LC-MS/MS). The optimal conditions for extraction of vitamin K(1) were material–liquid ratio of 1:70 (g/mL), extraction temperature of 50 °C, extraction power of 700 W, extraction time of 50 min, material-wash fluid ratio of 1:60 (g/mL), and 8 mL of hexane/anhydrous ether (97:3, v/v) as the elution solvent. Then, vitamin K(1) was analyzed on a ZORBAX SB-C18 column (50 mm × 2.1 mm, 1.8 μm) by gradient elution with water (0.01% formic acid) and methanol (0.01 formic acid + 2.5 mmol/L ammonium formate) as the mobile phase. The limit of detection (LOD) and limit of quantification (LOQ) were 0.05 and 0.16 μg/kg, respectively. Calibration curve was linear over the range of 10–500 ng/mL (R(2) > 0.9988). The recoveries at three spiked levels were between 80.9% and 119.1%. The validation and application indicated that the proposed method was simple and sensitive in determination of vitamin K(1) in fat-containing foods. |
format | Online Article Text |
id | pubmed-7070738 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-70707382020-03-19 Extraction and Determination of Vitamin K(1) in Foods by Ultrasound-Assisted Extraction, SPE, and LC-MS/MS Xu, Yueqing Zhang, Liangxiao Yang, Ruinan Yu, Xu Yu, Li Ma, Fei Li, Hui Wang, Xiupin Li, Peiwu Molecules Article Vitamin K(1) is one of the important hydrophobic vitamins in fat-containing foods. Traditionally, lipase is employed in the determination of vitamin K(1) to remove the lipids, which makes the detection complex, time-consuming, and insensitive. In this study, the determination of vitamin K(1) in fat-containing foods was developed based on ultrasound-assisted extraction (UAE), solid-phase extraction (SPE) combined with liquid chromatography–tandem mass spectrometry (LC-MS/MS). The optimal conditions for extraction of vitamin K(1) were material–liquid ratio of 1:70 (g/mL), extraction temperature of 50 °C, extraction power of 700 W, extraction time of 50 min, material-wash fluid ratio of 1:60 (g/mL), and 8 mL of hexane/anhydrous ether (97:3, v/v) as the elution solvent. Then, vitamin K(1) was analyzed on a ZORBAX SB-C18 column (50 mm × 2.1 mm, 1.8 μm) by gradient elution with water (0.01% formic acid) and methanol (0.01 formic acid + 2.5 mmol/L ammonium formate) as the mobile phase. The limit of detection (LOD) and limit of quantification (LOQ) were 0.05 and 0.16 μg/kg, respectively. Calibration curve was linear over the range of 10–500 ng/mL (R(2) > 0.9988). The recoveries at three spiked levels were between 80.9% and 119.1%. The validation and application indicated that the proposed method was simple and sensitive in determination of vitamin K(1) in fat-containing foods. MDPI 2020-02-14 /pmc/articles/PMC7070738/ /pubmed/32075057 http://dx.doi.org/10.3390/molecules25040839 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Xu, Yueqing Zhang, Liangxiao Yang, Ruinan Yu, Xu Yu, Li Ma, Fei Li, Hui Wang, Xiupin Li, Peiwu Extraction and Determination of Vitamin K(1) in Foods by Ultrasound-Assisted Extraction, SPE, and LC-MS/MS |
title | Extraction and Determination of Vitamin K(1) in Foods by Ultrasound-Assisted Extraction, SPE, and LC-MS/MS |
title_full | Extraction and Determination of Vitamin K(1) in Foods by Ultrasound-Assisted Extraction, SPE, and LC-MS/MS |
title_fullStr | Extraction and Determination of Vitamin K(1) in Foods by Ultrasound-Assisted Extraction, SPE, and LC-MS/MS |
title_full_unstemmed | Extraction and Determination of Vitamin K(1) in Foods by Ultrasound-Assisted Extraction, SPE, and LC-MS/MS |
title_short | Extraction and Determination of Vitamin K(1) in Foods by Ultrasound-Assisted Extraction, SPE, and LC-MS/MS |
title_sort | extraction and determination of vitamin k(1) in foods by ultrasound-assisted extraction, spe, and lc-ms/ms |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7070738/ https://www.ncbi.nlm.nih.gov/pubmed/32075057 http://dx.doi.org/10.3390/molecules25040839 |
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