Cargando…
Study on Cecropin B2 Production via Construct Bearing Intein Oligopeptide Cleavage Variants
In this study, genetic engineering was applied to the overexpression of the antimicrobial peptide (AMP) cecropin B2 (cecB2). pTWIN1 vector with a chitin-binding domain (CBD) and an auto-cleavage Ssp DnaB intein (INT) was coupled to the cecB2 to form a fusion protein construct and expressed via Esche...
Autores principales: | Fang, Yi-Ting, Li, Si-Yu, Hu, Nien-Jen, Yang, Jie, Liu, Jyung-Hurng, Liu, Yung-Chuan |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7070832/ https://www.ncbi.nlm.nih.gov/pubmed/32102349 http://dx.doi.org/10.3390/molecules25041005 |
Ejemplares similares
-
Antibacterial Peptide CecropinB2 Production via Various Host and Construct Systems
por: Lai, Wei-Shiang, et al.
Publicado: (2016) -
A novel secretion and online-cleavage strategy for production of cecropin A in Escherichia coli
por: Wang, Meng, et al.
Publicado: (2017) -
Protein Trans-Splicing of Multiple Atypical Split Inteins Engineered from Natural Inteins
por: Lin, Ying, et al.
Publicado: (2013) -
Semisynthesis of Protein variants Using Intein-Mediated Protein Ligation
por: Arnold, Ulrich, et al.
Publicado: (2001) -
Intein
Zymogens: Conditional Assembly and Splicing
of Split Inteins via Targeted Proteolysis
por: Gramespacher, Josef A., et al.
Publicado: (2017)