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Exploration of Rapid Evaporative-Ionization Mass Spectrometry as a Shotgun Approach for the Comprehensive Characterization of Kigelia Africana (Lam) Benth. Fruit

Rapid evaporative-ionization mass spectrometry (REIMS) coupled with an electroknife as a sampling device was recently employed in many application fields to obtain a rapid characterization of different samples without any need for extraction or cleanup procedures. In the present research, REIMS was...

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Autores principales: Arena, Katia, Rigano, Francesca, Mangraviti, Domenica, Cacciola, Francesco, Occhiuto, Francesco, Dugo, Laura, Dugo, Paola, Mondello, Luigi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7070896/
https://www.ncbi.nlm.nih.gov/pubmed/32093421
http://dx.doi.org/10.3390/molecules25040962
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author Arena, Katia
Rigano, Francesca
Mangraviti, Domenica
Cacciola, Francesco
Occhiuto, Francesco
Dugo, Laura
Dugo, Paola
Mondello, Luigi
author_facet Arena, Katia
Rigano, Francesca
Mangraviti, Domenica
Cacciola, Francesco
Occhiuto, Francesco
Dugo, Laura
Dugo, Paola
Mondello, Luigi
author_sort Arena, Katia
collection PubMed
description Rapid evaporative-ionization mass spectrometry (REIMS) coupled with an electroknife as a sampling device was recently employed in many application fields to obtain a rapid characterization of different samples without any need for extraction or cleanup procedures. In the present research, REIMS was used to obtain a metabolic profiling of the Kigelia africana fruit, thus extending the applicability of such a technique to the investigation of phytochemical constituents. In particular, the advantages of REIMS linked to a typical electrosurgical handpiece were applied for a comprehensive screening of this botanical species, by exploiting the mass accuracy and tandem MS capabilities of a quadrupole-time of flight analyzer. Then, 78 biomolecules were positively identified, including phenols, fatty acids and phospholipids. In the last decade, Kigelia africana (Lam.) Benth. fruit has attracted special interest for its drug-like properties, e.g., its use for infertility treatments and as anti-tumor agent, as well as against fungal and bacterial infections, diabetes, and inflammatory processes. Many of these properties are currently correlated to the presence of phenolic compounds, also detected in the present study, while the native lipid composition is here reported for the first time and could open new directions in the evaluation of therapeutic activity.
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spelling pubmed-70708962020-03-19 Exploration of Rapid Evaporative-Ionization Mass Spectrometry as a Shotgun Approach for the Comprehensive Characterization of Kigelia Africana (Lam) Benth. Fruit Arena, Katia Rigano, Francesca Mangraviti, Domenica Cacciola, Francesco Occhiuto, Francesco Dugo, Laura Dugo, Paola Mondello, Luigi Molecules Article Rapid evaporative-ionization mass spectrometry (REIMS) coupled with an electroknife as a sampling device was recently employed in many application fields to obtain a rapid characterization of different samples without any need for extraction or cleanup procedures. In the present research, REIMS was used to obtain a metabolic profiling of the Kigelia africana fruit, thus extending the applicability of such a technique to the investigation of phytochemical constituents. In particular, the advantages of REIMS linked to a typical electrosurgical handpiece were applied for a comprehensive screening of this botanical species, by exploiting the mass accuracy and tandem MS capabilities of a quadrupole-time of flight analyzer. Then, 78 biomolecules were positively identified, including phenols, fatty acids and phospholipids. In the last decade, Kigelia africana (Lam.) Benth. fruit has attracted special interest for its drug-like properties, e.g., its use for infertility treatments and as anti-tumor agent, as well as against fungal and bacterial infections, diabetes, and inflammatory processes. Many of these properties are currently correlated to the presence of phenolic compounds, also detected in the present study, while the native lipid composition is here reported for the first time and could open new directions in the evaluation of therapeutic activity. MDPI 2020-02-20 /pmc/articles/PMC7070896/ /pubmed/32093421 http://dx.doi.org/10.3390/molecules25040962 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Arena, Katia
Rigano, Francesca
Mangraviti, Domenica
Cacciola, Francesco
Occhiuto, Francesco
Dugo, Laura
Dugo, Paola
Mondello, Luigi
Exploration of Rapid Evaporative-Ionization Mass Spectrometry as a Shotgun Approach for the Comprehensive Characterization of Kigelia Africana (Lam) Benth. Fruit
title Exploration of Rapid Evaporative-Ionization Mass Spectrometry as a Shotgun Approach for the Comprehensive Characterization of Kigelia Africana (Lam) Benth. Fruit
title_full Exploration of Rapid Evaporative-Ionization Mass Spectrometry as a Shotgun Approach for the Comprehensive Characterization of Kigelia Africana (Lam) Benth. Fruit
title_fullStr Exploration of Rapid Evaporative-Ionization Mass Spectrometry as a Shotgun Approach for the Comprehensive Characterization of Kigelia Africana (Lam) Benth. Fruit
title_full_unstemmed Exploration of Rapid Evaporative-Ionization Mass Spectrometry as a Shotgun Approach for the Comprehensive Characterization of Kigelia Africana (Lam) Benth. Fruit
title_short Exploration of Rapid Evaporative-Ionization Mass Spectrometry as a Shotgun Approach for the Comprehensive Characterization of Kigelia Africana (Lam) Benth. Fruit
title_sort exploration of rapid evaporative-ionization mass spectrometry as a shotgun approach for the comprehensive characterization of kigelia africana (lam) benth. fruit
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7070896/
https://www.ncbi.nlm.nih.gov/pubmed/32093421
http://dx.doi.org/10.3390/molecules25040962
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