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Potency Assessment of CBD Oils by Their Effects on Cell Signaling Pathways

This study used nanofluidic protein posttranslational modification (PTM) profiling to measure the effects of six cannabidiol (CBD) oils and isolated CBD on the signaling pathways of a cultured SH-SY5Y neuronal cell line. Chemical composition analysis revealed that all CBD oils met the label claims a...

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Autores principales: Urasaki, Yasuyo, Beaumont, Cody, Workman, Michelle, Talbot, Jeffery N., Hill, David K., Le, Thuc T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7071207/
https://www.ncbi.nlm.nih.gov/pubmed/32019055
http://dx.doi.org/10.3390/nu12020357
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author Urasaki, Yasuyo
Beaumont, Cody
Workman, Michelle
Talbot, Jeffery N.
Hill, David K.
Le, Thuc T.
author_facet Urasaki, Yasuyo
Beaumont, Cody
Workman, Michelle
Talbot, Jeffery N.
Hill, David K.
Le, Thuc T.
author_sort Urasaki, Yasuyo
collection PubMed
description This study used nanofluidic protein posttranslational modification (PTM) profiling to measure the effects of six cannabidiol (CBD) oils and isolated CBD on the signaling pathways of a cultured SH-SY5Y neuronal cell line. Chemical composition analysis revealed that all CBD oils met the label claims and legal regulatory limit regarding the CBD and tetrahydrocannabinol (THC) contents, respectively. Isolated CBD was cytotoxic, with an effective concentration (EC(50)) of 40 µM. In contrast, the CBD oils had no effect on cell viability at CBD concentrations exceeding 1.2 mM. Interestingly, only an unadulterated CBD oil had strong and statistically significant suppressive effects on the pI3K/Akt/mTOR signaling pathway with an EC(50) value of 143 µM and a slow-acting timescale requiring hours. Systematic profiling of twenty-six proteins, which served as biomarkers for nine signaling pathways, revealed that the unadulterated CBD oil downregulated seven signaling pathways but had no measurable effect on the other two signaling pathways. The remaining CBD oils, which were adulterated, and isolated CBD had weak, variable, or undetectable effects on neuronal signaling pathways. Our data clearly showed that adulteration diminished the biological activities of CBD oils. In addition, nanofluidic protein PTM profiling provided a robust means for potency assessment of CBD oils.
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spelling pubmed-70712072020-03-19 Potency Assessment of CBD Oils by Their Effects on Cell Signaling Pathways Urasaki, Yasuyo Beaumont, Cody Workman, Michelle Talbot, Jeffery N. Hill, David K. Le, Thuc T. Nutrients Article This study used nanofluidic protein posttranslational modification (PTM) profiling to measure the effects of six cannabidiol (CBD) oils and isolated CBD on the signaling pathways of a cultured SH-SY5Y neuronal cell line. Chemical composition analysis revealed that all CBD oils met the label claims and legal regulatory limit regarding the CBD and tetrahydrocannabinol (THC) contents, respectively. Isolated CBD was cytotoxic, with an effective concentration (EC(50)) of 40 µM. In contrast, the CBD oils had no effect on cell viability at CBD concentrations exceeding 1.2 mM. Interestingly, only an unadulterated CBD oil had strong and statistically significant suppressive effects on the pI3K/Akt/mTOR signaling pathway with an EC(50) value of 143 µM and a slow-acting timescale requiring hours. Systematic profiling of twenty-six proteins, which served as biomarkers for nine signaling pathways, revealed that the unadulterated CBD oil downregulated seven signaling pathways but had no measurable effect on the other two signaling pathways. The remaining CBD oils, which were adulterated, and isolated CBD had weak, variable, or undetectable effects on neuronal signaling pathways. Our data clearly showed that adulteration diminished the biological activities of CBD oils. In addition, nanofluidic protein PTM profiling provided a robust means for potency assessment of CBD oils. MDPI 2020-01-30 /pmc/articles/PMC7071207/ /pubmed/32019055 http://dx.doi.org/10.3390/nu12020357 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Urasaki, Yasuyo
Beaumont, Cody
Workman, Michelle
Talbot, Jeffery N.
Hill, David K.
Le, Thuc T.
Potency Assessment of CBD Oils by Their Effects on Cell Signaling Pathways
title Potency Assessment of CBD Oils by Their Effects on Cell Signaling Pathways
title_full Potency Assessment of CBD Oils by Their Effects on Cell Signaling Pathways
title_fullStr Potency Assessment of CBD Oils by Their Effects on Cell Signaling Pathways
title_full_unstemmed Potency Assessment of CBD Oils by Their Effects on Cell Signaling Pathways
title_short Potency Assessment of CBD Oils by Their Effects on Cell Signaling Pathways
title_sort potency assessment of cbd oils by their effects on cell signaling pathways
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7071207/
https://www.ncbi.nlm.nih.gov/pubmed/32019055
http://dx.doi.org/10.3390/nu12020357
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