Cargando…

Erythrocyte Ascorbate Is a Potential Indicator of Steady-State Plasma Ascorbate Concentrations in Healthy Non-Fasting Individuals

Plasma vitamin C concentrations fluctuate in response to recent dietary intake; therefore levels are typically determined in the fasting state. Erythrocyte ascorbate concentrations have been shown to be similar to plasma levels, but little is known about the kinetics of ascorbate accumulation in the...

Descripción completa

Detalles Bibliográficos
Autores principales: M. Pullar, Juliet, Dunham, Susannah, U. Dachs, Gabi, C. M. Vissers, Margreet, C. Carr, Anitra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7071312/
https://www.ncbi.nlm.nih.gov/pubmed/32041092
http://dx.doi.org/10.3390/nu12020418
_version_ 1783506172966338560
author M. Pullar, Juliet
Dunham, Susannah
U. Dachs, Gabi
C. M. Vissers, Margreet
C. Carr, Anitra
author_facet M. Pullar, Juliet
Dunham, Susannah
U. Dachs, Gabi
C. M. Vissers, Margreet
C. Carr, Anitra
author_sort M. Pullar, Juliet
collection PubMed
description Plasma vitamin C concentrations fluctuate in response to recent dietary intake; therefore levels are typically determined in the fasting state. Erythrocyte ascorbate concentrations have been shown to be similar to plasma levels, but little is known about the kinetics of ascorbate accumulation in these cells. In this study, we investigated ascorbate uptake into erythrocytes after dietary supplementation with vitamin C and compared it to changes in plasma ascorbate concentrations. Seven individuals with baseline fasting plasma vitamin C concentrations ≥ 50 µmol/L were depleted of vitamin C-containing foods and drinks for one week, and then supplemented with 250 mg vitamin C/day in addition to resuming their normal diet. Fasting or steady-state plasma ascorbate concentrations declined to almost half of their baseline concentration over the week of vitamin C depletion, and then returned to saturation within two days of beginning supplementation. Erythrocyte ascorbate concentrations exhibited a very similar profile to plasma levels, with values ~76% of plasma, and a strong linear correlation (r = 0.89, p < 0.0001). Using a pharmacokinetic study design in six individuals with baseline fasting plasma vitamin C concentrations ≥50 µmol/L, we also showed that, unlike plasma, which peaked between 2 and 4 h following ingestion of 200 mg of vitamin C, erythrocyte ascorbate concentrations did not change in the six hours after supplementation. The data from these two intervention studies indicate that erythrocyte ascorbate concentration provides a stable measure of steady-state plasma ascorbate status and could be used to monitor ascorbate status in healthy non-fasting individuals.
format Online
Article
Text
id pubmed-7071312
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-70713122020-03-19 Erythrocyte Ascorbate Is a Potential Indicator of Steady-State Plasma Ascorbate Concentrations in Healthy Non-Fasting Individuals M. Pullar, Juliet Dunham, Susannah U. Dachs, Gabi C. M. Vissers, Margreet C. Carr, Anitra Nutrients Article Plasma vitamin C concentrations fluctuate in response to recent dietary intake; therefore levels are typically determined in the fasting state. Erythrocyte ascorbate concentrations have been shown to be similar to plasma levels, but little is known about the kinetics of ascorbate accumulation in these cells. In this study, we investigated ascorbate uptake into erythrocytes after dietary supplementation with vitamin C and compared it to changes in plasma ascorbate concentrations. Seven individuals with baseline fasting plasma vitamin C concentrations ≥ 50 µmol/L were depleted of vitamin C-containing foods and drinks for one week, and then supplemented with 250 mg vitamin C/day in addition to resuming their normal diet. Fasting or steady-state plasma ascorbate concentrations declined to almost half of their baseline concentration over the week of vitamin C depletion, and then returned to saturation within two days of beginning supplementation. Erythrocyte ascorbate concentrations exhibited a very similar profile to plasma levels, with values ~76% of plasma, and a strong linear correlation (r = 0.89, p < 0.0001). Using a pharmacokinetic study design in six individuals with baseline fasting plasma vitamin C concentrations ≥50 µmol/L, we also showed that, unlike plasma, which peaked between 2 and 4 h following ingestion of 200 mg of vitamin C, erythrocyte ascorbate concentrations did not change in the six hours after supplementation. The data from these two intervention studies indicate that erythrocyte ascorbate concentration provides a stable measure of steady-state plasma ascorbate status and could be used to monitor ascorbate status in healthy non-fasting individuals. MDPI 2020-02-06 /pmc/articles/PMC7071312/ /pubmed/32041092 http://dx.doi.org/10.3390/nu12020418 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
M. Pullar, Juliet
Dunham, Susannah
U. Dachs, Gabi
C. M. Vissers, Margreet
C. Carr, Anitra
Erythrocyte Ascorbate Is a Potential Indicator of Steady-State Plasma Ascorbate Concentrations in Healthy Non-Fasting Individuals
title Erythrocyte Ascorbate Is a Potential Indicator of Steady-State Plasma Ascorbate Concentrations in Healthy Non-Fasting Individuals
title_full Erythrocyte Ascorbate Is a Potential Indicator of Steady-State Plasma Ascorbate Concentrations in Healthy Non-Fasting Individuals
title_fullStr Erythrocyte Ascorbate Is a Potential Indicator of Steady-State Plasma Ascorbate Concentrations in Healthy Non-Fasting Individuals
title_full_unstemmed Erythrocyte Ascorbate Is a Potential Indicator of Steady-State Plasma Ascorbate Concentrations in Healthy Non-Fasting Individuals
title_short Erythrocyte Ascorbate Is a Potential Indicator of Steady-State Plasma Ascorbate Concentrations in Healthy Non-Fasting Individuals
title_sort erythrocyte ascorbate is a potential indicator of steady-state plasma ascorbate concentrations in healthy non-fasting individuals
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7071312/
https://www.ncbi.nlm.nih.gov/pubmed/32041092
http://dx.doi.org/10.3390/nu12020418
work_keys_str_mv AT mpullarjuliet erythrocyteascorbateisapotentialindicatorofsteadystateplasmaascorbateconcentrationsinhealthynonfastingindividuals
AT dunhamsusannah erythrocyteascorbateisapotentialindicatorofsteadystateplasmaascorbateconcentrationsinhealthynonfastingindividuals
AT udachsgabi erythrocyteascorbateisapotentialindicatorofsteadystateplasmaascorbateconcentrationsinhealthynonfastingindividuals
AT cmvissersmargreet erythrocyteascorbateisapotentialindicatorofsteadystateplasmaascorbateconcentrationsinhealthynonfastingindividuals
AT ccarranitra erythrocyteascorbateisapotentialindicatorofsteadystateplasmaascorbateconcentrationsinhealthynonfastingindividuals