Cargando…

Secretome Analysis of Mouse Dendritic Cells Interacting with a Probiotic Strain of Lactobacillus gasseri

Probiotics play a key role in the modulation of the gut immune system in health and disease and their action is mediated by molecules exposed on the microorganism surface or secreted probiotic-derived factors. In particular, Lactobacillus gasseri OLL2809, a probiotic microorganism isolated from huma...

Descripción completa

Detalles Bibliográficos
Autores principales: Mazzeo, Maria Fiorella, Luongo, Diomira, Sashihara, Toshihiro, Rossi, Mauro, Siciliano, Rosa Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7071482/
https://www.ncbi.nlm.nih.gov/pubmed/32093322
http://dx.doi.org/10.3390/nu12020555
_version_ 1783506212219781120
author Mazzeo, Maria Fiorella
Luongo, Diomira
Sashihara, Toshihiro
Rossi, Mauro
Siciliano, Rosa Anna
author_facet Mazzeo, Maria Fiorella
Luongo, Diomira
Sashihara, Toshihiro
Rossi, Mauro
Siciliano, Rosa Anna
author_sort Mazzeo, Maria Fiorella
collection PubMed
description Probiotics play a key role in the modulation of the gut immune system in health and disease and their action is mediated by molecules exposed on the microorganism surface or secreted probiotic-derived factors. In particular, Lactobacillus gasseri OLL2809, a probiotic microorganism isolated from human feces, has the potential to modulate various immune responses. The dendritic cells (DCs) are considered the main players in orchestrating the immune response, and their contact with intestinal microbiota is crucial for the development and homeostasis of gut immunity. To gain a perspective on the molecular mechanisms involved in the maturation process of DCs and investigate factors that could modulate these processes, a differential proteomic analysis was performed on the secretome of immature DCs, mature DCs (mDCs, induced by lipopolysaccharide (LPS)), and immature DCs challenged with L. gasseri OLL2809 before treatment with LPS (LGmDCs). The maturation process of DCs was associated to profound changes in the protein secretome and probiotic pre-treatment led to a dramatic modulation of several secreted proteins of mDC, not only classical immune mediators (i.e., cytokines, complement factors, T cell Receptor ligands) but also proteins involved in the contractile and desmosome machineries. The latter data highlight a novel mechanism by which L. gasseri can modulate the maturation process of DCs, reinforcing the concept of a protective anti-inflammatory role ascribed to this probiotic strain.
format Online
Article
Text
id pubmed-7071482
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-70714822020-03-19 Secretome Analysis of Mouse Dendritic Cells Interacting with a Probiotic Strain of Lactobacillus gasseri Mazzeo, Maria Fiorella Luongo, Diomira Sashihara, Toshihiro Rossi, Mauro Siciliano, Rosa Anna Nutrients Article Probiotics play a key role in the modulation of the gut immune system in health and disease and their action is mediated by molecules exposed on the microorganism surface or secreted probiotic-derived factors. In particular, Lactobacillus gasseri OLL2809, a probiotic microorganism isolated from human feces, has the potential to modulate various immune responses. The dendritic cells (DCs) are considered the main players in orchestrating the immune response, and their contact with intestinal microbiota is crucial for the development and homeostasis of gut immunity. To gain a perspective on the molecular mechanisms involved in the maturation process of DCs and investigate factors that could modulate these processes, a differential proteomic analysis was performed on the secretome of immature DCs, mature DCs (mDCs, induced by lipopolysaccharide (LPS)), and immature DCs challenged with L. gasseri OLL2809 before treatment with LPS (LGmDCs). The maturation process of DCs was associated to profound changes in the protein secretome and probiotic pre-treatment led to a dramatic modulation of several secreted proteins of mDC, not only classical immune mediators (i.e., cytokines, complement factors, T cell Receptor ligands) but also proteins involved in the contractile and desmosome machineries. The latter data highlight a novel mechanism by which L. gasseri can modulate the maturation process of DCs, reinforcing the concept of a protective anti-inflammatory role ascribed to this probiotic strain. MDPI 2020-02-20 /pmc/articles/PMC7071482/ /pubmed/32093322 http://dx.doi.org/10.3390/nu12020555 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mazzeo, Maria Fiorella
Luongo, Diomira
Sashihara, Toshihiro
Rossi, Mauro
Siciliano, Rosa Anna
Secretome Analysis of Mouse Dendritic Cells Interacting with a Probiotic Strain of Lactobacillus gasseri
title Secretome Analysis of Mouse Dendritic Cells Interacting with a Probiotic Strain of Lactobacillus gasseri
title_full Secretome Analysis of Mouse Dendritic Cells Interacting with a Probiotic Strain of Lactobacillus gasseri
title_fullStr Secretome Analysis of Mouse Dendritic Cells Interacting with a Probiotic Strain of Lactobacillus gasseri
title_full_unstemmed Secretome Analysis of Mouse Dendritic Cells Interacting with a Probiotic Strain of Lactobacillus gasseri
title_short Secretome Analysis of Mouse Dendritic Cells Interacting with a Probiotic Strain of Lactobacillus gasseri
title_sort secretome analysis of mouse dendritic cells interacting with a probiotic strain of lactobacillus gasseri
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7071482/
https://www.ncbi.nlm.nih.gov/pubmed/32093322
http://dx.doi.org/10.3390/nu12020555
work_keys_str_mv AT mazzeomariafiorella secretomeanalysisofmousedendriticcellsinteractingwithaprobioticstrainoflactobacillusgasseri
AT luongodiomira secretomeanalysisofmousedendriticcellsinteractingwithaprobioticstrainoflactobacillusgasseri
AT sashiharatoshihiro secretomeanalysisofmousedendriticcellsinteractingwithaprobioticstrainoflactobacillusgasseri
AT rossimauro secretomeanalysisofmousedendriticcellsinteractingwithaprobioticstrainoflactobacillusgasseri
AT sicilianorosaanna secretomeanalysisofmousedendriticcellsinteractingwithaprobioticstrainoflactobacillusgasseri