Cargando…

A simple plant high-molecular-weight DNA extraction method suitable for single-molecule technologies

BACKGROUND: High-molecular-weight and pure DNA is crucial for high-quality results from 3rd generation DNA Analyzers and optical mapping technologies. Conventional nuclei isolation methods for preparing high-molecular-weight genomic DNA from plant tissues include the preparation of protoplasts or em...

Descripción completa

Detalles Bibliográficos
Autores principales:	Li, Zhigang, Parris, Stephen, Saski, Christopher A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2020
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7071634/ https://www.ncbi.nlm.nih.gov/pubmed/32190102 http://dx.doi.org/10.1186/s13007-020-00579-4

Ejemplares similares

Protocol: a versatile, inexpensive, high-throughput plant genomic DNA extraction method suitable for genotyping-by-sequencing
por: Anderson, Craig B., et al.
Publicado: (2018)

Protocol: A high-throughput DNA extraction system suitable for conifers
por: Bashalkhanov, Stanislav, et al.
Publicado: (2008)

Suitable transfection methods for single particle tracing in plant suspension cells
por: Göhring, Janett, et al.
Publicado: (2014)

SILEX: a fast and inexpensive high-quality DNA extraction method suitable for multiple sequencing platforms and recalcitrant plant species
por: Vilanova, Santiago, et al.
Publicado: (2020)

A high quality, high molecular weight DNA extraction method for PacBio HiFi genome sequencing of recalcitrant plants
por: Nishii, Kanae, et al.
Publicado: (2023)

Protocol: a simple method for extracting next-generation sequencing quality genomic DNA from recalcitrant plant species
por: Healey, Adam, et al.
Publicado: (2014)

An improved method with a wider applicability to isolate plant mitochondria for mtDNA extraction
por: Ahmed, Zaheer, et al.
Publicado: (2015)

DNA-free high-quality RNA extraction from 39 difficult-to-extract plant species (representing seasonal tissues and tissue types) of 32 families, and its validation for downstream molecular applications
por: Sasi, Shina, et al.
Publicado: (2023)

Assessment of differences between DNA content of cell-cultured and freely suspended oocysts of Cryptosporidium parvum and their suitability as DNA standards in qPCR
por: Woolsey, Ian D., et al.
Publicado: (2019)

Improving the performance of true single molecule sequencing for ancient DNA
por: Ginolhac, Aurelien, et al.
Publicado: (2012)

A rapid and sensitive method for the simultaneous analysis of aliphatic and polar molecules containing free carboxyl groups in plant extracts by LC-MS/MS
por: Kallenbach, Mario, et al.
Publicado: (2009)

An integrated platform for bovine DNA methylome analysis suitable for small samples
por: Shojaei Saadi, Habib A, et al.
Publicado: (2014)

Application of FTA technology for sampling, recovery and molecular characterization of viral pathogens and virus-derived transgenes from plant tissues
por: Ndunguru, Joseph, et al.
Publicado: (2005)

A simple and efficient method for the long-term preservation of plant cell suspension cultures
por: Boisson, Anne-Marie, et al.
Publicado: (2012)

A simple and efficient method for isolating small RNAs from different plant species
por: Rosas-Cárdenas, Flor de Fátima, et al.
Publicado: (2011)

Establishment of an efficient cotton root protoplast isolation protocol suitable for single-cell RNA sequencing and transient gene expression analysis
por: Zhang, Ke, et al.
Publicado: (2023)

Protocol: a fast and simple in situ PCR method for localising gene expression in plant tissue
por: Athman, Asmini, et al.
Publicado: (2014)

Simple and efficient methods to generate split roots and grafted plants useful for long-distance signaling studies in Medicago truncatula and other small plants
por: Kassaw, Tessema K, et al.
Publicado: (2012)

A comparison of semi-quantitative methods suitable for establishing volatile profiles
por: Ruiz-Hernández, Victoria, et al.
Publicado: (2018)

A fully automatable enzymatic method for DNA extraction from plant tissues
por: Manen, Jean-François, et al.
Publicado: (2005)

Analysis of RNA base modification and structural rearrangement by single-molecule real-time detection of reverse transcription
por: Vilfan, Igor D, et al.
Publicado: (2013)

A method for detecting single mRNA molecules in Arabidopsis thaliana
por: Duncan, Susan, et al.
Publicado: (2016)

INC-Seq: accurate single molecule reads using nanopore sequencing
por: Li, Chenhao, et al.
Publicado: (2016)

Single-molecule quantification of 5-hydroxymethylcytosine for diagnosis of blood and colon cancers
por: Gilat, Noa, et al.
Publicado: (2017)

Protocol: A simple phenol-based method for 96-well extraction of high quality RNA from Arabidopsis
por: Box, Mathew S, et al.
Publicado: (2011)

Simple extraction methods that prevent the artifactual conversion of chlorophyll to chlorophyllide during pigment isolation from leaf samples
por: Hu, Xueyun, et al.
Publicado: (2013)

A simple and efficient micrografting method for stably transformed Nicotiana attenuata plants to examine shoot-root signaling
por: Fragoso, Variluska, et al.
Publicado: (2011)

An efficient method for high molecular weight bacterial DNA extraction suitable for shotgun metagenomics from skin swabs
por: Serghiou, Iliana R., et al.
Publicado: (2023)

An improved method of constructing degradome library suitable for sequencing using Illumina platform
por: Li, Yong-Fang, et al.
Publicado: (2019)

Simultaneous molecular formula determinations of natural compounds in a plant extract using 15 T Fourier transform ion cyclotron resonance mass spectrometry
por: Park, Kyu Hwan, et al.
Publicado: (2013)

The plant leaf movement analyzer (PALMA): a simple tool for the analysis of periodic cotyledon and leaf movement in Arabidopsis thaliana
por: Wagner, Lucas, et al.
Publicado: (2017)

Rapid and reliable extraction of genomic DNA from various wild-type and transgenic plants
por: Kang, Tae-Jin, et al.
Publicado: (2004)

Advances in Molecular Imprinting Technology for the Extraction and Detection of Quercetin in Plants
por: Ye, Kexi, et al.
Publicado: (2023)

Optimization of DNA extraction for advancing coral microbiota investigations
por: Weber, Laura, et al.
Publicado: (2017)

Selection of a protein solubilization method suitable for phytopathogenic bacteria: a proteomics approach
por: Malafaia, Carolina B, et al.
Publicado: (2015)

Protocol: a simple gel-free method for SNP genotyping using allele-specific primers in rice and other plant species
por: Hirotsu, Naoki, et al.
Publicado: (2010)

Fluctuating light experiments and semi-automated plant phenotyping enabled by self-built growth racks and simple upgrades to the IMAGING-PAM
por: Schneider, Dominik, et al.
Publicado: (2019)

A simple and robust method for connecting small-molecule drugs using gene-expression signatures
por: Zhang, Shu-Dong, et al.
Publicado: (2008)

Studies on cellulase-ultrasonic assisted extraction technology for flavonoids from Illicium verum residues
por: Huang, Danna, et al.
Publicado: (2016)

Protocol: a rapid and economical procedure for purification of plasmid or plant DNA with diverse applications in plant biology
por: Li, Jian-Feng, et al.
Publicado: (2010)

Cannot write session to /tmp/vufind_sessions/sess_tk0j28r7cmv6oglp6f42n30b4m