A rapid and simple method for routine determination of antibiotic sensitivity to biofilm populations of Pseudomonas aeruginosa

Treatment of infections by Pseudomonas aeruginosa forming biofilms after antimicrobial testing on planktonic bacteria can result in substantial failure. Therefore, we offer a robust and simple experimental platform to test the impact of antimicrobials on biofilms. Antibiotic response patterns varied...

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Autores principales: Wannigama, Dhammika Leshan, Hurst, Cameron, Hongsing, Parichart, Pearson, Lachlan, Saethang, Thammakorn, Chantaravisoot, Naphat, Singkham-in, Uthaibhorn, Luk-in, Sirirat, Storer, Robin James, Chatsuwan, Tanittha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7071750/
https://www.ncbi.nlm.nih.gov/pubmed/32169075
http://dx.doi.org/10.1186/s12941-020-00350-6
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author Wannigama, Dhammika Leshan
Hurst, Cameron
Hongsing, Parichart
Pearson, Lachlan
Saethang, Thammakorn
Chantaravisoot, Naphat
Singkham-in, Uthaibhorn
Luk-in, Sirirat
Storer, Robin James
Chatsuwan, Tanittha
author_facet Wannigama, Dhammika Leshan
Hurst, Cameron
Hongsing, Parichart
Pearson, Lachlan
Saethang, Thammakorn
Chantaravisoot, Naphat
Singkham-in, Uthaibhorn
Luk-in, Sirirat
Storer, Robin James
Chatsuwan, Tanittha
author_sort Wannigama, Dhammika Leshan
collection PubMed
description Treatment of infections by Pseudomonas aeruginosa forming biofilms after antimicrobial testing on planktonic bacteria can result in substantial failure. Therefore, we offer a robust and simple experimental platform to test the impact of antimicrobials on biofilms. Antibiotic response patterns varied uniquely within biofilm formation capacity and minimal biofilm eradication concentrations (MBECs) has a significantly better discriminatory power than minimum inhibitory concentrations (MICs) to differentiate the overall efficiency of antibiotics to eradicate biofilm. Our resazurin-based 96-well-plate platform is able to emulate bacterial responses to antibiotics under biofilm conditions in a fast, simple, and cost-effective screening method adaptable to automation, and warrants trials in the clinic.
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spelling pubmed-70717502020-03-18 A rapid and simple method for routine determination of antibiotic sensitivity to biofilm populations of Pseudomonas aeruginosa Wannigama, Dhammika Leshan Hurst, Cameron Hongsing, Parichart Pearson, Lachlan Saethang, Thammakorn Chantaravisoot, Naphat Singkham-in, Uthaibhorn Luk-in, Sirirat Storer, Robin James Chatsuwan, Tanittha Ann Clin Microbiol Antimicrob Short Report Treatment of infections by Pseudomonas aeruginosa forming biofilms after antimicrobial testing on planktonic bacteria can result in substantial failure. Therefore, we offer a robust and simple experimental platform to test the impact of antimicrobials on biofilms. Antibiotic response patterns varied uniquely within biofilm formation capacity and minimal biofilm eradication concentrations (MBECs) has a significantly better discriminatory power than minimum inhibitory concentrations (MICs) to differentiate the overall efficiency of antibiotics to eradicate biofilm. Our resazurin-based 96-well-plate platform is able to emulate bacterial responses to antibiotics under biofilm conditions in a fast, simple, and cost-effective screening method adaptable to automation, and warrants trials in the clinic. BioMed Central 2020-03-13 /pmc/articles/PMC7071750/ /pubmed/32169075 http://dx.doi.org/10.1186/s12941-020-00350-6 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Short Report
Wannigama, Dhammika Leshan
Hurst, Cameron
Hongsing, Parichart
Pearson, Lachlan
Saethang, Thammakorn
Chantaravisoot, Naphat
Singkham-in, Uthaibhorn
Luk-in, Sirirat
Storer, Robin James
Chatsuwan, Tanittha
A rapid and simple method for routine determination of antibiotic sensitivity to biofilm populations of Pseudomonas aeruginosa
title A rapid and simple method for routine determination of antibiotic sensitivity to biofilm populations of Pseudomonas aeruginosa
title_full A rapid and simple method for routine determination of antibiotic sensitivity to biofilm populations of Pseudomonas aeruginosa
title_fullStr A rapid and simple method for routine determination of antibiotic sensitivity to biofilm populations of Pseudomonas aeruginosa
title_full_unstemmed A rapid and simple method for routine determination of antibiotic sensitivity to biofilm populations of Pseudomonas aeruginosa
title_short A rapid and simple method for routine determination of antibiotic sensitivity to biofilm populations of Pseudomonas aeruginosa
title_sort rapid and simple method for routine determination of antibiotic sensitivity to biofilm populations of pseudomonas aeruginosa
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7071750/
https://www.ncbi.nlm.nih.gov/pubmed/32169075
http://dx.doi.org/10.1186/s12941-020-00350-6
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