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Application of an antibody chip for screening differentially expressed proteins during peach ripening and identification of a metabolon in the SAM cycle to generate a peach ethylene biosynthesis model

Peach (Prunus persica) is a typical climacteric fruit that produces ethylene rapidly during ripening, and its fruit softens quickly. Stony hard peach cultivars, however, do not produce large amounts of ethylene, and the fruit remains firm until fully ripe, thus differing from melting flesh peach cul...

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Autores principales: Zeng, Wenfang, Niu, Liang, Wang, Zhaohui, Wang, Xiaobei, Wang, Yan, Pan, Lei, Lu, Zhenhua, Cui, Guochao, Weng, Weining, Wang, Mingqiao, Meng, Xun, Wang, Zhiqiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7072073/
https://www.ncbi.nlm.nih.gov/pubmed/32194967
http://dx.doi.org/10.1038/s41438-020-0249-9
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author Zeng, Wenfang
Niu, Liang
Wang, Zhaohui
Wang, Xiaobei
Wang, Yan
Pan, Lei
Lu, Zhenhua
Cui, Guochao
Weng, Weining
Wang, Mingqiao
Meng, Xun
Wang, Zhiqiang
author_facet Zeng, Wenfang
Niu, Liang
Wang, Zhaohui
Wang, Xiaobei
Wang, Yan
Pan, Lei
Lu, Zhenhua
Cui, Guochao
Weng, Weining
Wang, Mingqiao
Meng, Xun
Wang, Zhiqiang
author_sort Zeng, Wenfang
collection PubMed
description Peach (Prunus persica) is a typical climacteric fruit that produces ethylene rapidly during ripening, and its fruit softens quickly. Stony hard peach cultivars, however, do not produce large amounts of ethylene, and the fruit remains firm until fully ripe, thus differing from melting flesh peach cultivars. To identify the key proteins involved in peach fruit ripening, an antibody-based proteomic analysis was conducted. A mega-monoclonal antibody (mAb) library was generated and arrayed on a chip (mAbArray) at a high density, covering ~4950 different proteins of peach. Through the screening of peach fruit proteins with the mAbArray chip, differentially expressed proteins recognized by 1587 mAbs were identified, and 33 corresponding antigens were ultimately identified by immunoprecipitation and mass spectrometry. These proteins included not only important enzymes involved in ethylene biosynthesis, such as ACO1, SAHH, SAMS, and MetE, but also novel factors such as NUDT2. Furthermore, protein–protein interaction analysis identified a metabolon containing SAHH and MetE. By combining the antibody-based proteomic data with the transcriptomic and metabolic data, a mathematical model of ethylene biosynthesis in peach was constructed. Simulation results showed that MetE is an important regulator during peach ripening, partially through interaction with SAHH.
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spelling pubmed-70720732020-03-19 Application of an antibody chip for screening differentially expressed proteins during peach ripening and identification of a metabolon in the SAM cycle to generate a peach ethylene biosynthesis model Zeng, Wenfang Niu, Liang Wang, Zhaohui Wang, Xiaobei Wang, Yan Pan, Lei Lu, Zhenhua Cui, Guochao Weng, Weining Wang, Mingqiao Meng, Xun Wang, Zhiqiang Hortic Res Article Peach (Prunus persica) is a typical climacteric fruit that produces ethylene rapidly during ripening, and its fruit softens quickly. Stony hard peach cultivars, however, do not produce large amounts of ethylene, and the fruit remains firm until fully ripe, thus differing from melting flesh peach cultivars. To identify the key proteins involved in peach fruit ripening, an antibody-based proteomic analysis was conducted. A mega-monoclonal antibody (mAb) library was generated and arrayed on a chip (mAbArray) at a high density, covering ~4950 different proteins of peach. Through the screening of peach fruit proteins with the mAbArray chip, differentially expressed proteins recognized by 1587 mAbs were identified, and 33 corresponding antigens were ultimately identified by immunoprecipitation and mass spectrometry. These proteins included not only important enzymes involved in ethylene biosynthesis, such as ACO1, SAHH, SAMS, and MetE, but also novel factors such as NUDT2. Furthermore, protein–protein interaction analysis identified a metabolon containing SAHH and MetE. By combining the antibody-based proteomic data with the transcriptomic and metabolic data, a mathematical model of ethylene biosynthesis in peach was constructed. Simulation results showed that MetE is an important regulator during peach ripening, partially through interaction with SAHH. Nature Publishing Group UK 2020-03-15 /pmc/articles/PMC7072073/ /pubmed/32194967 http://dx.doi.org/10.1038/s41438-020-0249-9 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Zeng, Wenfang
Niu, Liang
Wang, Zhaohui
Wang, Xiaobei
Wang, Yan
Pan, Lei
Lu, Zhenhua
Cui, Guochao
Weng, Weining
Wang, Mingqiao
Meng, Xun
Wang, Zhiqiang
Application of an antibody chip for screening differentially expressed proteins during peach ripening and identification of a metabolon in the SAM cycle to generate a peach ethylene biosynthesis model
title Application of an antibody chip for screening differentially expressed proteins during peach ripening and identification of a metabolon in the SAM cycle to generate a peach ethylene biosynthesis model
title_full Application of an antibody chip for screening differentially expressed proteins during peach ripening and identification of a metabolon in the SAM cycle to generate a peach ethylene biosynthesis model
title_fullStr Application of an antibody chip for screening differentially expressed proteins during peach ripening and identification of a metabolon in the SAM cycle to generate a peach ethylene biosynthesis model
title_full_unstemmed Application of an antibody chip for screening differentially expressed proteins during peach ripening and identification of a metabolon in the SAM cycle to generate a peach ethylene biosynthesis model
title_short Application of an antibody chip for screening differentially expressed proteins during peach ripening and identification of a metabolon in the SAM cycle to generate a peach ethylene biosynthesis model
title_sort application of an antibody chip for screening differentially expressed proteins during peach ripening and identification of a metabolon in the sam cycle to generate a peach ethylene biosynthesis model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7072073/
https://www.ncbi.nlm.nih.gov/pubmed/32194967
http://dx.doi.org/10.1038/s41438-020-0249-9
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