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Octacalcium phosphate/gelatin composite facilitates bone regeneration of critical-sized mandibular defects in rats: A quantitative study

Background. Regeneration of bone defects remains a challenge for maxillofacial surgeons. The present study aimed to compare the effects of octacalcium phosphate (OCP) and the combination of octacalcium phosphate/gelatin (OCP/Gel) on mandibular bone regeneration in rats Methods. In the present study,...

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Autores principales: Sargolzaei-Aval, Fereydoon, Saberi, Eshagh Ali, Arab, Mohammad Reza, Sargolzaei, Narjes, Sanchooli, Tayebeh, Tavakolinezhad, Sima
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tabriz University of Medical Sciences 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7072084/
https://www.ncbi.nlm.nih.gov/pubmed/32190209
http://dx.doi.org/10.15171/joddd.2019.040
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author Sargolzaei-Aval, Fereydoon
Saberi, Eshagh Ali
Arab, Mohammad Reza
Sargolzaei, Narjes
Sanchooli, Tayebeh
Tavakolinezhad, Sima
author_facet Sargolzaei-Aval, Fereydoon
Saberi, Eshagh Ali
Arab, Mohammad Reza
Sargolzaei, Narjes
Sanchooli, Tayebeh
Tavakolinezhad, Sima
author_sort Sargolzaei-Aval, Fereydoon
collection PubMed
description Background. Regeneration of bone defects remains a challenge for maxillofacial surgeons. The present study aimed to compare the effects of octacalcium phosphate (OCP) and the combination of octacalcium phosphate/gelatin (OCP/Gel) on mandibular bone regeneration in rats Methods. In the present study, 36 male Sprague-Dawley rats were used. The animals were randomly assigned to the following experimental groups: OCP (n=12), OCP/Gel (n=12), and the control group (n=12). Defects were created in the rat mandibles and filled with 10 mg of OCP and OCP/Gel disks in the experimental groups. In the control group, however, no substance was administered. Samples were taken on days 7, 14, 21 and 56, respectively, after the implantation. Sections (5 µ) were prepared and stained by H&E. The sections were studied, and the volume fraction of newly formed bone was measured by Dunnett's T3 test based on the significance level (P=0.05). Results. In the experimental groups, the new bone formation began from the margin of defects 7‒14 days after the implantation. During the healing process, the newly formed bone healed a larger area of the defects and grew structurally. In the control group, the defects were primarily filled with dense connective tissue, and only a small amount of new bone was formed. The present study showed a statistically significant difference in the volume of newly formed bone between the experimental groups and the control group (P<0.001). Conclusion. OCP/Gel composite can be beneficial in the healing process of mandibular bone defects.
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spelling pubmed-70720842020-03-18 Octacalcium phosphate/gelatin composite facilitates bone regeneration of critical-sized mandibular defects in rats: A quantitative study Sargolzaei-Aval, Fereydoon Saberi, Eshagh Ali Arab, Mohammad Reza Sargolzaei, Narjes Sanchooli, Tayebeh Tavakolinezhad, Sima J Dent Res Dent Clin Dent Prospects Original Research Background. Regeneration of bone defects remains a challenge for maxillofacial surgeons. The present study aimed to compare the effects of octacalcium phosphate (OCP) and the combination of octacalcium phosphate/gelatin (OCP/Gel) on mandibular bone regeneration in rats Methods. In the present study, 36 male Sprague-Dawley rats were used. The animals were randomly assigned to the following experimental groups: OCP (n=12), OCP/Gel (n=12), and the control group (n=12). Defects were created in the rat mandibles and filled with 10 mg of OCP and OCP/Gel disks in the experimental groups. In the control group, however, no substance was administered. Samples were taken on days 7, 14, 21 and 56, respectively, after the implantation. Sections (5 µ) were prepared and stained by H&E. The sections were studied, and the volume fraction of newly formed bone was measured by Dunnett's T3 test based on the significance level (P=0.05). Results. In the experimental groups, the new bone formation began from the margin of defects 7‒14 days after the implantation. During the healing process, the newly formed bone healed a larger area of the defects and grew structurally. In the control group, the defects were primarily filled with dense connective tissue, and only a small amount of new bone was formed. The present study showed a statistically significant difference in the volume of newly formed bone between the experimental groups and the control group (P<0.001). Conclusion. OCP/Gel composite can be beneficial in the healing process of mandibular bone defects. Tabriz University of Medical Sciences 2019 /pmc/articles/PMC7072084/ /pubmed/32190209 http://dx.doi.org/10.15171/joddd.2019.040 Text en © 2019 Sargolzaei-Aval et al. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article published and distributed by Tabriz University of Medical Sciences under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Sargolzaei-Aval, Fereydoon
Saberi, Eshagh Ali
Arab, Mohammad Reza
Sargolzaei, Narjes
Sanchooli, Tayebeh
Tavakolinezhad, Sima
Octacalcium phosphate/gelatin composite facilitates bone regeneration of critical-sized mandibular defects in rats: A quantitative study
title Octacalcium phosphate/gelatin composite facilitates bone regeneration of critical-sized mandibular defects in rats: A quantitative study
title_full Octacalcium phosphate/gelatin composite facilitates bone regeneration of critical-sized mandibular defects in rats: A quantitative study
title_fullStr Octacalcium phosphate/gelatin composite facilitates bone regeneration of critical-sized mandibular defects in rats: A quantitative study
title_full_unstemmed Octacalcium phosphate/gelatin composite facilitates bone regeneration of critical-sized mandibular defects in rats: A quantitative study
title_short Octacalcium phosphate/gelatin composite facilitates bone regeneration of critical-sized mandibular defects in rats: A quantitative study
title_sort octacalcium phosphate/gelatin composite facilitates bone regeneration of critical-sized mandibular defects in rats: a quantitative study
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7072084/
https://www.ncbi.nlm.nih.gov/pubmed/32190209
http://dx.doi.org/10.15171/joddd.2019.040
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