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Meniscus Matrix Remodeling in Response to Compressive Forces in Dogs

Joint motion and postnatal stress of weight bearing are the principal factors that determine the phenotypical and architectural changes that characterize the maturation process of the meniscus. In this study, the effect of compressive forces on the meniscus will be evaluated in a litter of 12 Doberm...

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Autores principales: Polito, Umberto, Peretti, Giuseppe M., Di Giancamillo, Mauro, Boschetti, Federica, Carnevale, Liliana, Veronesi, Maria C., Sconfienza, Luca M., Agnoletto, Marco, Mangiavini, Laura, Modina, Silvia C., Di Giancamillo, Alessia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7072134/
https://www.ncbi.nlm.nih.gov/pubmed/31973209
http://dx.doi.org/10.3390/cells9020265
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author Polito, Umberto
Peretti, Giuseppe M.
Di Giancamillo, Mauro
Boschetti, Federica
Carnevale, Liliana
Veronesi, Maria C.
Sconfienza, Luca M.
Agnoletto, Marco
Mangiavini, Laura
Modina, Silvia C.
Di Giancamillo, Alessia
author_facet Polito, Umberto
Peretti, Giuseppe M.
Di Giancamillo, Mauro
Boschetti, Federica
Carnevale, Liliana
Veronesi, Maria C.
Sconfienza, Luca M.
Agnoletto, Marco
Mangiavini, Laura
Modina, Silvia C.
Di Giancamillo, Alessia
author_sort Polito, Umberto
collection PubMed
description Joint motion and postnatal stress of weight bearing are the principal factors that determine the phenotypical and architectural changes that characterize the maturation process of the meniscus. In this study, the effect of compressive forces on the meniscus will be evaluated in a litter of 12 Dobermann Pinschers, of approximately 2 months of age, euthanized as affected by the quadriceps contracture muscle syndrome of a single limb focusing on extracellular matrix remodeling and cell–extracellular matrix interaction (i.e., meniscal cells maturation, collagen fibers typology and arrangement). The affected limbs were considered as models of continuous compression while the physiologic loaded limbs were considered as controls. The results of this study suggest that a compressive continuous force, applied to the native meniscal cells, triggers an early maturation of the cellular phenotype, at the expense of the proper organization of collagen fibers. Nevertheless, an application of a compressive force could be useful in the engineering process of meniscal tissue in order to induce a faster achievement of the mature cellular phenotype and, consequently, the earlier production of the fundamental extracellular matrix (ECM), in order to improve cellular viability and adhesion of the cells within a hypothetical synthetic scaffold.
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spelling pubmed-70721342020-03-19 Meniscus Matrix Remodeling in Response to Compressive Forces in Dogs Polito, Umberto Peretti, Giuseppe M. Di Giancamillo, Mauro Boschetti, Federica Carnevale, Liliana Veronesi, Maria C. Sconfienza, Luca M. Agnoletto, Marco Mangiavini, Laura Modina, Silvia C. Di Giancamillo, Alessia Cells Article Joint motion and postnatal stress of weight bearing are the principal factors that determine the phenotypical and architectural changes that characterize the maturation process of the meniscus. In this study, the effect of compressive forces on the meniscus will be evaluated in a litter of 12 Dobermann Pinschers, of approximately 2 months of age, euthanized as affected by the quadriceps contracture muscle syndrome of a single limb focusing on extracellular matrix remodeling and cell–extracellular matrix interaction (i.e., meniscal cells maturation, collagen fibers typology and arrangement). The affected limbs were considered as models of continuous compression while the physiologic loaded limbs were considered as controls. The results of this study suggest that a compressive continuous force, applied to the native meniscal cells, triggers an early maturation of the cellular phenotype, at the expense of the proper organization of collagen fibers. Nevertheless, an application of a compressive force could be useful in the engineering process of meniscal tissue in order to induce a faster achievement of the mature cellular phenotype and, consequently, the earlier production of the fundamental extracellular matrix (ECM), in order to improve cellular viability and adhesion of the cells within a hypothetical synthetic scaffold. MDPI 2020-01-21 /pmc/articles/PMC7072134/ /pubmed/31973209 http://dx.doi.org/10.3390/cells9020265 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Polito, Umberto
Peretti, Giuseppe M.
Di Giancamillo, Mauro
Boschetti, Federica
Carnevale, Liliana
Veronesi, Maria C.
Sconfienza, Luca M.
Agnoletto, Marco
Mangiavini, Laura
Modina, Silvia C.
Di Giancamillo, Alessia
Meniscus Matrix Remodeling in Response to Compressive Forces in Dogs
title Meniscus Matrix Remodeling in Response to Compressive Forces in Dogs
title_full Meniscus Matrix Remodeling in Response to Compressive Forces in Dogs
title_fullStr Meniscus Matrix Remodeling in Response to Compressive Forces in Dogs
title_full_unstemmed Meniscus Matrix Remodeling in Response to Compressive Forces in Dogs
title_short Meniscus Matrix Remodeling in Response to Compressive Forces in Dogs
title_sort meniscus matrix remodeling in response to compressive forces in dogs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7072134/
https://www.ncbi.nlm.nih.gov/pubmed/31973209
http://dx.doi.org/10.3390/cells9020265
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