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Q-Cell Glioblastoma Resource: Proteomics Analysis Reveals Unique Cell-States Are Maintained in 3D Culture

Glioblastoma (GBM) is a treatment-refractory central nervous system (CNS) tumour, and better therapies to treat this aggressive disease are urgently needed. Primary GBM models that represent the true disease state are essential to better understand disease biology and for accurate preclinical therap...

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Autores principales: D’Souza, Rochelle C. J., Offenhäuser, Carolin, Straube, Jasmin, Baumgartner, Ulrich, Kordowski, Anja, Li, Yuchen, Stringer, Brett W., Alexander, Hamish, Lwin, Zarnie, Inglis, Po-Ling, Jeffree, Rosalind L., Johns, Terrance G., Boyd, Andrew W., Day, Bryan W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7072469/
https://www.ncbi.nlm.nih.gov/pubmed/31973233
http://dx.doi.org/10.3390/cells9020267
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author D’Souza, Rochelle C. J.
Offenhäuser, Carolin
Straube, Jasmin
Baumgartner, Ulrich
Kordowski, Anja
Li, Yuchen
Stringer, Brett W.
Alexander, Hamish
Lwin, Zarnie
Inglis, Po-Ling
Jeffree, Rosalind L.
Johns, Terrance G.
Boyd, Andrew W.
Day, Bryan W.
author_facet D’Souza, Rochelle C. J.
Offenhäuser, Carolin
Straube, Jasmin
Baumgartner, Ulrich
Kordowski, Anja
Li, Yuchen
Stringer, Brett W.
Alexander, Hamish
Lwin, Zarnie
Inglis, Po-Ling
Jeffree, Rosalind L.
Johns, Terrance G.
Boyd, Andrew W.
Day, Bryan W.
author_sort D’Souza, Rochelle C. J.
collection PubMed
description Glioblastoma (GBM) is a treatment-refractory central nervous system (CNS) tumour, and better therapies to treat this aggressive disease are urgently needed. Primary GBM models that represent the true disease state are essential to better understand disease biology and for accurate preclinical therapy assessment. We have previously presented a comprehensive transcriptome characterisation of a panel (n = 12) of primary GBM models (Q-Cell). We have now generated a systematic, quantitative, and deep proteome abundance atlas of the Q-Cell models grown in 3D culture, representing 6167 human proteins. A recent study has highlighted the degree of functional heterogeneity that coexists within individual GBM tumours, describing four cellular states (MES-like, NPC-like, OPC-like and AC-like). We performed comparative proteomic analysis, confirming a good representation of each of the four cell-states across the 13 models examined. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis identified upregulation of a number of GBM-associated cancer pathway proteins. Bioinformatics analysis, using the OncoKB database, identified a number of functional actionable targets that were either uniquely or ubiquitously expressed across the panel. This study provides an in-depth proteomic analysis of the GBM Q-Cell resource, which should prove a valuable functional dataset for future biological and preclinical investigations.
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spelling pubmed-70724692020-03-19 Q-Cell Glioblastoma Resource: Proteomics Analysis Reveals Unique Cell-States Are Maintained in 3D Culture D’Souza, Rochelle C. J. Offenhäuser, Carolin Straube, Jasmin Baumgartner, Ulrich Kordowski, Anja Li, Yuchen Stringer, Brett W. Alexander, Hamish Lwin, Zarnie Inglis, Po-Ling Jeffree, Rosalind L. Johns, Terrance G. Boyd, Andrew W. Day, Bryan W. Cells Article Glioblastoma (GBM) is a treatment-refractory central nervous system (CNS) tumour, and better therapies to treat this aggressive disease are urgently needed. Primary GBM models that represent the true disease state are essential to better understand disease biology and for accurate preclinical therapy assessment. We have previously presented a comprehensive transcriptome characterisation of a panel (n = 12) of primary GBM models (Q-Cell). We have now generated a systematic, quantitative, and deep proteome abundance atlas of the Q-Cell models grown in 3D culture, representing 6167 human proteins. A recent study has highlighted the degree of functional heterogeneity that coexists within individual GBM tumours, describing four cellular states (MES-like, NPC-like, OPC-like and AC-like). We performed comparative proteomic analysis, confirming a good representation of each of the four cell-states across the 13 models examined. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis identified upregulation of a number of GBM-associated cancer pathway proteins. Bioinformatics analysis, using the OncoKB database, identified a number of functional actionable targets that were either uniquely or ubiquitously expressed across the panel. This study provides an in-depth proteomic analysis of the GBM Q-Cell resource, which should prove a valuable functional dataset for future biological and preclinical investigations. MDPI 2020-01-21 /pmc/articles/PMC7072469/ /pubmed/31973233 http://dx.doi.org/10.3390/cells9020267 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
D’Souza, Rochelle C. J.
Offenhäuser, Carolin
Straube, Jasmin
Baumgartner, Ulrich
Kordowski, Anja
Li, Yuchen
Stringer, Brett W.
Alexander, Hamish
Lwin, Zarnie
Inglis, Po-Ling
Jeffree, Rosalind L.
Johns, Terrance G.
Boyd, Andrew W.
Day, Bryan W.
Q-Cell Glioblastoma Resource: Proteomics Analysis Reveals Unique Cell-States Are Maintained in 3D Culture
title Q-Cell Glioblastoma Resource: Proteomics Analysis Reveals Unique Cell-States Are Maintained in 3D Culture
title_full Q-Cell Glioblastoma Resource: Proteomics Analysis Reveals Unique Cell-States Are Maintained in 3D Culture
title_fullStr Q-Cell Glioblastoma Resource: Proteomics Analysis Reveals Unique Cell-States Are Maintained in 3D Culture
title_full_unstemmed Q-Cell Glioblastoma Resource: Proteomics Analysis Reveals Unique Cell-States Are Maintained in 3D Culture
title_short Q-Cell Glioblastoma Resource: Proteomics Analysis Reveals Unique Cell-States Are Maintained in 3D Culture
title_sort q-cell glioblastoma resource: proteomics analysis reveals unique cell-states are maintained in 3d culture
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7072469/
https://www.ncbi.nlm.nih.gov/pubmed/31973233
http://dx.doi.org/10.3390/cells9020267
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