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Metabolic and Proteomic Perspectives of Augmentation of Nutritional Contents and Plant Defense in Vigna unguiculata

The current study enlists metabolites of Alstonia scholaris with bioactivities, and the most active compound, 3-(1-methylpyrrolidin-2-yl) pyridine, was selected against Macrophomina phaseolina. Appraisal of the Alstonia metabolites identified the 3-(1-methylpyrrolidin-2-yl) pyridine as a bioactive c...

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Autores principales: Ahmad, Aqeel, Khan, Tanveer Alam, Mubeen, Samavia, Shahzadi, Iqra, Akram, Waheed, Saeed, Taiba, Bashir, Zoobia, Wang, Rui, Alam, Mufid, Ahmed, Shakeel, Hu, Du, Li, Guihua, Wu, Tingquan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7072685/
https://www.ncbi.nlm.nih.gov/pubmed/32028654
http://dx.doi.org/10.3390/biom10020224
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author Ahmad, Aqeel
Khan, Tanveer Alam
Mubeen, Samavia
Shahzadi, Iqra
Akram, Waheed
Saeed, Taiba
Bashir, Zoobia
Wang, Rui
Alam, Mufid
Ahmed, Shakeel
Hu, Du
Li, Guihua
Wu, Tingquan
author_facet Ahmad, Aqeel
Khan, Tanveer Alam
Mubeen, Samavia
Shahzadi, Iqra
Akram, Waheed
Saeed, Taiba
Bashir, Zoobia
Wang, Rui
Alam, Mufid
Ahmed, Shakeel
Hu, Du
Li, Guihua
Wu, Tingquan
author_sort Ahmad, Aqeel
collection PubMed
description The current study enlists metabolites of Alstonia scholaris with bioactivities, and the most active compound, 3-(1-methylpyrrolidin-2-yl) pyridine, was selected against Macrophomina phaseolina. Appraisal of the Alstonia metabolites identified the 3-(1-methylpyrrolidin-2-yl) pyridine as a bioactive compound which elevated vitamins and nutritional contents of Vigna unguiculata up to ≥18%, and other physiological parameters up to 28.9%. The bioactive compound (0.1%) upregulated key defense genes, shifted defense metabolism from salicylic acid to jasmonic acid, and induced glucanase enzymes for improved defenses. The structural studies categorized four glucanase-isozymes under beta-glycanases falling in (Trans) glycosidases with TIM beta/alpha-barrel fold. The study determined key-protein factors (Q9SAJ4) for elevated nutritional contents, along with its structural and functional mechanisms, as well as interactions with other loci. The nicotine-docked Q9SAJ4 protein showed a 200% elevated activity and interacted with AT1G79550.2, AT1G12900.1, AT1G13440.1, AT3G04120.1, and AT3G26650.1 loci to ramp up the metabolic processes. Furthermore, the study emphasizes the physiological mechanism involved in the enrichment of the nutritional contents of V. unguiculata. Metabolic studies concluded that increased melibiose and glucose 6-phosphate contents, accompanied by reduced trehalose (-0.9-fold), with sugar drifts to downstream pyruvate biosynthesis and acetyl Co-A metabolism mainly triggered nutritional contents. Hydrogen bonding at residues G.357, G.380, and G.381 docked nicotine with Q9SAJ4 and transformed its bilobed structure for easy exposure toward substrate molecules. The current study augments the nutritional value of edible stuff and supports agriculture-based country economies.
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spelling pubmed-70726852020-03-19 Metabolic and Proteomic Perspectives of Augmentation of Nutritional Contents and Plant Defense in Vigna unguiculata Ahmad, Aqeel Khan, Tanveer Alam Mubeen, Samavia Shahzadi, Iqra Akram, Waheed Saeed, Taiba Bashir, Zoobia Wang, Rui Alam, Mufid Ahmed, Shakeel Hu, Du Li, Guihua Wu, Tingquan Biomolecules Article The current study enlists metabolites of Alstonia scholaris with bioactivities, and the most active compound, 3-(1-methylpyrrolidin-2-yl) pyridine, was selected against Macrophomina phaseolina. Appraisal of the Alstonia metabolites identified the 3-(1-methylpyrrolidin-2-yl) pyridine as a bioactive compound which elevated vitamins and nutritional contents of Vigna unguiculata up to ≥18%, and other physiological parameters up to 28.9%. The bioactive compound (0.1%) upregulated key defense genes, shifted defense metabolism from salicylic acid to jasmonic acid, and induced glucanase enzymes for improved defenses. The structural studies categorized four glucanase-isozymes under beta-glycanases falling in (Trans) glycosidases with TIM beta/alpha-barrel fold. The study determined key-protein factors (Q9SAJ4) for elevated nutritional contents, along with its structural and functional mechanisms, as well as interactions with other loci. The nicotine-docked Q9SAJ4 protein showed a 200% elevated activity and interacted with AT1G79550.2, AT1G12900.1, AT1G13440.1, AT3G04120.1, and AT3G26650.1 loci to ramp up the metabolic processes. Furthermore, the study emphasizes the physiological mechanism involved in the enrichment of the nutritional contents of V. unguiculata. Metabolic studies concluded that increased melibiose and glucose 6-phosphate contents, accompanied by reduced trehalose (-0.9-fold), with sugar drifts to downstream pyruvate biosynthesis and acetyl Co-A metabolism mainly triggered nutritional contents. Hydrogen bonding at residues G.357, G.380, and G.381 docked nicotine with Q9SAJ4 and transformed its bilobed structure for easy exposure toward substrate molecules. The current study augments the nutritional value of edible stuff and supports agriculture-based country economies. MDPI 2020-02-03 /pmc/articles/PMC7072685/ /pubmed/32028654 http://dx.doi.org/10.3390/biom10020224 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ahmad, Aqeel
Khan, Tanveer Alam
Mubeen, Samavia
Shahzadi, Iqra
Akram, Waheed
Saeed, Taiba
Bashir, Zoobia
Wang, Rui
Alam, Mufid
Ahmed, Shakeel
Hu, Du
Li, Guihua
Wu, Tingquan
Metabolic and Proteomic Perspectives of Augmentation of Nutritional Contents and Plant Defense in Vigna unguiculata
title Metabolic and Proteomic Perspectives of Augmentation of Nutritional Contents and Plant Defense in Vigna unguiculata
title_full Metabolic and Proteomic Perspectives of Augmentation of Nutritional Contents and Plant Defense in Vigna unguiculata
title_fullStr Metabolic and Proteomic Perspectives of Augmentation of Nutritional Contents and Plant Defense in Vigna unguiculata
title_full_unstemmed Metabolic and Proteomic Perspectives of Augmentation of Nutritional Contents and Plant Defense in Vigna unguiculata
title_short Metabolic and Proteomic Perspectives of Augmentation of Nutritional Contents and Plant Defense in Vigna unguiculata
title_sort metabolic and proteomic perspectives of augmentation of nutritional contents and plant defense in vigna unguiculata
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7072685/
https://www.ncbi.nlm.nih.gov/pubmed/32028654
http://dx.doi.org/10.3390/biom10020224
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