Cargando…
Metabolic and Proteomic Perspectives of Augmentation of Nutritional Contents and Plant Defense in Vigna unguiculata
The current study enlists metabolites of Alstonia scholaris with bioactivities, and the most active compound, 3-(1-methylpyrrolidin-2-yl) pyridine, was selected against Macrophomina phaseolina. Appraisal of the Alstonia metabolites identified the 3-(1-methylpyrrolidin-2-yl) pyridine as a bioactive c...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7072685/ https://www.ncbi.nlm.nih.gov/pubmed/32028654 http://dx.doi.org/10.3390/biom10020224 |
_version_ | 1783506464128630784 |
---|---|
author | Ahmad, Aqeel Khan, Tanveer Alam Mubeen, Samavia Shahzadi, Iqra Akram, Waheed Saeed, Taiba Bashir, Zoobia Wang, Rui Alam, Mufid Ahmed, Shakeel Hu, Du Li, Guihua Wu, Tingquan |
author_facet | Ahmad, Aqeel Khan, Tanveer Alam Mubeen, Samavia Shahzadi, Iqra Akram, Waheed Saeed, Taiba Bashir, Zoobia Wang, Rui Alam, Mufid Ahmed, Shakeel Hu, Du Li, Guihua Wu, Tingquan |
author_sort | Ahmad, Aqeel |
collection | PubMed |
description | The current study enlists metabolites of Alstonia scholaris with bioactivities, and the most active compound, 3-(1-methylpyrrolidin-2-yl) pyridine, was selected against Macrophomina phaseolina. Appraisal of the Alstonia metabolites identified the 3-(1-methylpyrrolidin-2-yl) pyridine as a bioactive compound which elevated vitamins and nutritional contents of Vigna unguiculata up to ≥18%, and other physiological parameters up to 28.9%. The bioactive compound (0.1%) upregulated key defense genes, shifted defense metabolism from salicylic acid to jasmonic acid, and induced glucanase enzymes for improved defenses. The structural studies categorized four glucanase-isozymes under beta-glycanases falling in (Trans) glycosidases with TIM beta/alpha-barrel fold. The study determined key-protein factors (Q9SAJ4) for elevated nutritional contents, along with its structural and functional mechanisms, as well as interactions with other loci. The nicotine-docked Q9SAJ4 protein showed a 200% elevated activity and interacted with AT1G79550.2, AT1G12900.1, AT1G13440.1, AT3G04120.1, and AT3G26650.1 loci to ramp up the metabolic processes. Furthermore, the study emphasizes the physiological mechanism involved in the enrichment of the nutritional contents of V. unguiculata. Metabolic studies concluded that increased melibiose and glucose 6-phosphate contents, accompanied by reduced trehalose (-0.9-fold), with sugar drifts to downstream pyruvate biosynthesis and acetyl Co-A metabolism mainly triggered nutritional contents. Hydrogen bonding at residues G.357, G.380, and G.381 docked nicotine with Q9SAJ4 and transformed its bilobed structure for easy exposure toward substrate molecules. The current study augments the nutritional value of edible stuff and supports agriculture-based country economies. |
format | Online Article Text |
id | pubmed-7072685 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-70726852020-03-19 Metabolic and Proteomic Perspectives of Augmentation of Nutritional Contents and Plant Defense in Vigna unguiculata Ahmad, Aqeel Khan, Tanveer Alam Mubeen, Samavia Shahzadi, Iqra Akram, Waheed Saeed, Taiba Bashir, Zoobia Wang, Rui Alam, Mufid Ahmed, Shakeel Hu, Du Li, Guihua Wu, Tingquan Biomolecules Article The current study enlists metabolites of Alstonia scholaris with bioactivities, and the most active compound, 3-(1-methylpyrrolidin-2-yl) pyridine, was selected against Macrophomina phaseolina. Appraisal of the Alstonia metabolites identified the 3-(1-methylpyrrolidin-2-yl) pyridine as a bioactive compound which elevated vitamins and nutritional contents of Vigna unguiculata up to ≥18%, and other physiological parameters up to 28.9%. The bioactive compound (0.1%) upregulated key defense genes, shifted defense metabolism from salicylic acid to jasmonic acid, and induced glucanase enzymes for improved defenses. The structural studies categorized four glucanase-isozymes under beta-glycanases falling in (Trans) glycosidases with TIM beta/alpha-barrel fold. The study determined key-protein factors (Q9SAJ4) for elevated nutritional contents, along with its structural and functional mechanisms, as well as interactions with other loci. The nicotine-docked Q9SAJ4 protein showed a 200% elevated activity and interacted with AT1G79550.2, AT1G12900.1, AT1G13440.1, AT3G04120.1, and AT3G26650.1 loci to ramp up the metabolic processes. Furthermore, the study emphasizes the physiological mechanism involved in the enrichment of the nutritional contents of V. unguiculata. Metabolic studies concluded that increased melibiose and glucose 6-phosphate contents, accompanied by reduced trehalose (-0.9-fold), with sugar drifts to downstream pyruvate biosynthesis and acetyl Co-A metabolism mainly triggered nutritional contents. Hydrogen bonding at residues G.357, G.380, and G.381 docked nicotine with Q9SAJ4 and transformed its bilobed structure for easy exposure toward substrate molecules. The current study augments the nutritional value of edible stuff and supports agriculture-based country economies. MDPI 2020-02-03 /pmc/articles/PMC7072685/ /pubmed/32028654 http://dx.doi.org/10.3390/biom10020224 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ahmad, Aqeel Khan, Tanveer Alam Mubeen, Samavia Shahzadi, Iqra Akram, Waheed Saeed, Taiba Bashir, Zoobia Wang, Rui Alam, Mufid Ahmed, Shakeel Hu, Du Li, Guihua Wu, Tingquan Metabolic and Proteomic Perspectives of Augmentation of Nutritional Contents and Plant Defense in Vigna unguiculata |
title | Metabolic and Proteomic Perspectives of Augmentation of Nutritional Contents and Plant Defense in Vigna unguiculata |
title_full | Metabolic and Proteomic Perspectives of Augmentation of Nutritional Contents and Plant Defense in Vigna unguiculata |
title_fullStr | Metabolic and Proteomic Perspectives of Augmentation of Nutritional Contents and Plant Defense in Vigna unguiculata |
title_full_unstemmed | Metabolic and Proteomic Perspectives of Augmentation of Nutritional Contents and Plant Defense in Vigna unguiculata |
title_short | Metabolic and Proteomic Perspectives of Augmentation of Nutritional Contents and Plant Defense in Vigna unguiculata |
title_sort | metabolic and proteomic perspectives of augmentation of nutritional contents and plant defense in vigna unguiculata |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7072685/ https://www.ncbi.nlm.nih.gov/pubmed/32028654 http://dx.doi.org/10.3390/biom10020224 |
work_keys_str_mv | AT ahmadaqeel metabolicandproteomicperspectivesofaugmentationofnutritionalcontentsandplantdefenseinvignaunguiculata AT khantanveeralam metabolicandproteomicperspectivesofaugmentationofnutritionalcontentsandplantdefenseinvignaunguiculata AT mubeensamavia metabolicandproteomicperspectivesofaugmentationofnutritionalcontentsandplantdefenseinvignaunguiculata AT shahzadiiqra metabolicandproteomicperspectivesofaugmentationofnutritionalcontentsandplantdefenseinvignaunguiculata AT akramwaheed metabolicandproteomicperspectivesofaugmentationofnutritionalcontentsandplantdefenseinvignaunguiculata AT saeedtaiba metabolicandproteomicperspectivesofaugmentationofnutritionalcontentsandplantdefenseinvignaunguiculata AT bashirzoobia metabolicandproteomicperspectivesofaugmentationofnutritionalcontentsandplantdefenseinvignaunguiculata AT wangrui metabolicandproteomicperspectivesofaugmentationofnutritionalcontentsandplantdefenseinvignaunguiculata AT alammufid metabolicandproteomicperspectivesofaugmentationofnutritionalcontentsandplantdefenseinvignaunguiculata AT ahmedshakeel metabolicandproteomicperspectivesofaugmentationofnutritionalcontentsandplantdefenseinvignaunguiculata AT hudu metabolicandproteomicperspectivesofaugmentationofnutritionalcontentsandplantdefenseinvignaunguiculata AT liguihua metabolicandproteomicperspectivesofaugmentationofnutritionalcontentsandplantdefenseinvignaunguiculata AT wutingquan metabolicandproteomicperspectivesofaugmentationofnutritionalcontentsandplantdefenseinvignaunguiculata |