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Induction of Cryptic Antifungal Pulicatin Derivatives from Pantoea Agglomerans by Microbial Co-Culture

Microbial co-culture or mixed fermentation proved to be an efficient strategy to expand chemical diversity by the induction of cryptic biosynthetic pathways, and in many cases led to the production of new antimicrobial agents. In the current study, we report a rare example of the induction of silent...

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Autores principales: Thissera, Bathini, Alhadrami, Hani A., Hassan, Marwa H. A., Hassan, Hossam M., Behery, Fathy A., Bawazeer, Majed, Yaseen, Mohammed, Belbahri, Lassaad, Rateb, Mostafa E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7072716/
https://www.ncbi.nlm.nih.gov/pubmed/32050703
http://dx.doi.org/10.3390/biom10020268
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author Thissera, Bathini
Alhadrami, Hani A.
Hassan, Marwa H. A.
Hassan, Hossam M.
Behery, Fathy A.
Bawazeer, Majed
Yaseen, Mohammed
Belbahri, Lassaad
Rateb, Mostafa E.
author_facet Thissera, Bathini
Alhadrami, Hani A.
Hassan, Marwa H. A.
Hassan, Hossam M.
Behery, Fathy A.
Bawazeer, Majed
Yaseen, Mohammed
Belbahri, Lassaad
Rateb, Mostafa E.
author_sort Thissera, Bathini
collection PubMed
description Microbial co-culture or mixed fermentation proved to be an efficient strategy to expand chemical diversity by the induction of cryptic biosynthetic pathways, and in many cases led to the production of new antimicrobial agents. In the current study, we report a rare example of the induction of silent/cryptic bacterial biosynthetic pathway by the co-culture of Durum wheat plant roots-associated bacterium Pantoea aggolomerans and date palm leaves-derived fungus Penicillium citrinum. The initial co-culture indicated a clear fungal growth inhibition which was confirmed by the promising antifungal activity of the co-culture total extract against Pc. LC-HRMS chemical profiling demonstrated a huge suppression in the production of secondary metabolites (SMs) of axenic cultures of both species with the emergence of new metabolites which were dereplicated as a series of siderophores. Large-scale co-culture fermentation led to the isolation of two new pulicatin derivatives together with six known metabolites which were characterised using HRESIMS and NMR analyses. During the in vitro antimicrobial evaluation of the isolated compounds, pulicatin H (2) exhibited the strongest antifungal activity against Pc, followed by aeruginaldehyde (1) and pulicatin F (4), hence explaining the initial growth suppression of Pc in the co-culture environment.
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spelling pubmed-70727162020-03-19 Induction of Cryptic Antifungal Pulicatin Derivatives from Pantoea Agglomerans by Microbial Co-Culture Thissera, Bathini Alhadrami, Hani A. Hassan, Marwa H. A. Hassan, Hossam M. Behery, Fathy A. Bawazeer, Majed Yaseen, Mohammed Belbahri, Lassaad Rateb, Mostafa E. Biomolecules Article Microbial co-culture or mixed fermentation proved to be an efficient strategy to expand chemical diversity by the induction of cryptic biosynthetic pathways, and in many cases led to the production of new antimicrobial agents. In the current study, we report a rare example of the induction of silent/cryptic bacterial biosynthetic pathway by the co-culture of Durum wheat plant roots-associated bacterium Pantoea aggolomerans and date palm leaves-derived fungus Penicillium citrinum. The initial co-culture indicated a clear fungal growth inhibition which was confirmed by the promising antifungal activity of the co-culture total extract against Pc. LC-HRMS chemical profiling demonstrated a huge suppression in the production of secondary metabolites (SMs) of axenic cultures of both species with the emergence of new metabolites which were dereplicated as a series of siderophores. Large-scale co-culture fermentation led to the isolation of two new pulicatin derivatives together with six known metabolites which were characterised using HRESIMS and NMR analyses. During the in vitro antimicrobial evaluation of the isolated compounds, pulicatin H (2) exhibited the strongest antifungal activity against Pc, followed by aeruginaldehyde (1) and pulicatin F (4), hence explaining the initial growth suppression of Pc in the co-culture environment. MDPI 2020-02-10 /pmc/articles/PMC7072716/ /pubmed/32050703 http://dx.doi.org/10.3390/biom10020268 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Thissera, Bathini
Alhadrami, Hani A.
Hassan, Marwa H. A.
Hassan, Hossam M.
Behery, Fathy A.
Bawazeer, Majed
Yaseen, Mohammed
Belbahri, Lassaad
Rateb, Mostafa E.
Induction of Cryptic Antifungal Pulicatin Derivatives from Pantoea Agglomerans by Microbial Co-Culture
title Induction of Cryptic Antifungal Pulicatin Derivatives from Pantoea Agglomerans by Microbial Co-Culture
title_full Induction of Cryptic Antifungal Pulicatin Derivatives from Pantoea Agglomerans by Microbial Co-Culture
title_fullStr Induction of Cryptic Antifungal Pulicatin Derivatives from Pantoea Agglomerans by Microbial Co-Culture
title_full_unstemmed Induction of Cryptic Antifungal Pulicatin Derivatives from Pantoea Agglomerans by Microbial Co-Culture
title_short Induction of Cryptic Antifungal Pulicatin Derivatives from Pantoea Agglomerans by Microbial Co-Culture
title_sort induction of cryptic antifungal pulicatin derivatives from pantoea agglomerans by microbial co-culture
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7072716/
https://www.ncbi.nlm.nih.gov/pubmed/32050703
http://dx.doi.org/10.3390/biom10020268
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