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Dynamic Genome Editing Using In Vivo Synthesized Donor ssDNA in Escherichia coli

As a key element of genome editing, donor DNA introduces the desired exogenous sequence while working with other crucial machinery such as CRISPR-Cas or recombinases. However, current methods for the delivery of donor DNA into cells are both inefficient and complicated. Here, we developed a new meth...

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Detalles Bibliográficos
Autores principales: Hao, Min, Wang, Zhaoguan, Qiao, Hongyan, Yin, Peng, Qiao, Jianjun, Qi, Hao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7072734/
https://www.ncbi.nlm.nih.gov/pubmed/32085579
http://dx.doi.org/10.3390/cells9020467