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Dynamic Genome Editing Using In Vivo Synthesized Donor ssDNA in Escherichia coli
As a key element of genome editing, donor DNA introduces the desired exogenous sequence while working with other crucial machinery such as CRISPR-Cas or recombinases. However, current methods for the delivery of donor DNA into cells are both inefficient and complicated. Here, we developed a new meth...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
MDPI
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7072734/ https://www.ncbi.nlm.nih.gov/pubmed/32085579 http://dx.doi.org/10.3390/cells9020467 |