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Encoding Information into Polyethylene Glycol Using an Alcohol-Isocyanate “Click” Reaction

In this article, the capability of encoding information using a homologous series of monodisperse monomethoxypolyethylene glycols (mPEG), with a number of ethylene oxide units ranging from n(EO) = 5 to 8, and monodisperse linear aliphatic isocyanates containing a number of CH(2) units from 3 to 7, i...

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Autores principales: Nagy, Lajos, Kuki, Ákos, Nagy, Tibor, Vadkerti, Bence, Erdélyi, Zoltán, Kárpáti, Levente, Zsuga, Miklós, Kéki, Sándor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7072859/
https://www.ncbi.nlm.nih.gov/pubmed/32075293
http://dx.doi.org/10.3390/ijms21041318
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author Nagy, Lajos
Kuki, Ákos
Nagy, Tibor
Vadkerti, Bence
Erdélyi, Zoltán
Kárpáti, Levente
Zsuga, Miklós
Kéki, Sándor
author_facet Nagy, Lajos
Kuki, Ákos
Nagy, Tibor
Vadkerti, Bence
Erdélyi, Zoltán
Kárpáti, Levente
Zsuga, Miklós
Kéki, Sándor
author_sort Nagy, Lajos
collection PubMed
description In this article, the capability of encoding information using a homologous series of monodisperse monomethoxypolyethylene glycols (mPEG), with a number of ethylene oxide units ranging from n(EO) = 5 to 8, and monodisperse linear aliphatic isocyanates containing a number of CH(2) units from 3 to 7, is demonstrated. The “click” reaction of the two corresponding homologous series yielded 20 different isocyanate end-capped polyethylene glycol derivatives (mPEG-OCONHR) whose sodiated adduct ion’s nominal m/z values spanned from 360 to 548, providing an average ca. 8 m/z unit for the storage of one-bit information. These mPEG-OCONHR oligomers were then used to encode information in binary sequences using a 384-well MALDI sample plate and employing the common dried-droplet sample preparation method capable of encoding 20 bit, i.e., 2.5 byte information in one spot, was employed. The information stored in the spots was read by MALDI-TOF MS using the m/z value of the corresponding mPEG-OCONHR oligomers. The capability of the method to store data was demonstrated by writing and reading a text file, visualizing a small picture and capturing a short audio file written in Musical Instrument Digital Interface (MIDI) sequence. Due to the very large similarities in the chemical structures of the encoding oligomers and their “easy to be ionized” property, as well as their very similar ionization efficiencies, the MALDI-TOF MS signal intensities from each compound was so strong and unambiguous that complete decoding could be performed in each case. In addition, the set of the proposed encoding oligomers can be further extended to attain higher bit “densities”.
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spelling pubmed-70728592020-03-19 Encoding Information into Polyethylene Glycol Using an Alcohol-Isocyanate “Click” Reaction Nagy, Lajos Kuki, Ákos Nagy, Tibor Vadkerti, Bence Erdélyi, Zoltán Kárpáti, Levente Zsuga, Miklós Kéki, Sándor Int J Mol Sci Article In this article, the capability of encoding information using a homologous series of monodisperse monomethoxypolyethylene glycols (mPEG), with a number of ethylene oxide units ranging from n(EO) = 5 to 8, and monodisperse linear aliphatic isocyanates containing a number of CH(2) units from 3 to 7, is demonstrated. The “click” reaction of the two corresponding homologous series yielded 20 different isocyanate end-capped polyethylene glycol derivatives (mPEG-OCONHR) whose sodiated adduct ion’s nominal m/z values spanned from 360 to 548, providing an average ca. 8 m/z unit for the storage of one-bit information. These mPEG-OCONHR oligomers were then used to encode information in binary sequences using a 384-well MALDI sample plate and employing the common dried-droplet sample preparation method capable of encoding 20 bit, i.e., 2.5 byte information in one spot, was employed. The information stored in the spots was read by MALDI-TOF MS using the m/z value of the corresponding mPEG-OCONHR oligomers. The capability of the method to store data was demonstrated by writing and reading a text file, visualizing a small picture and capturing a short audio file written in Musical Instrument Digital Interface (MIDI) sequence. Due to the very large similarities in the chemical structures of the encoding oligomers and their “easy to be ionized” property, as well as their very similar ionization efficiencies, the MALDI-TOF MS signal intensities from each compound was so strong and unambiguous that complete decoding could be performed in each case. In addition, the set of the proposed encoding oligomers can be further extended to attain higher bit “densities”. MDPI 2020-02-15 /pmc/articles/PMC7072859/ /pubmed/32075293 http://dx.doi.org/10.3390/ijms21041318 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nagy, Lajos
Kuki, Ákos
Nagy, Tibor
Vadkerti, Bence
Erdélyi, Zoltán
Kárpáti, Levente
Zsuga, Miklós
Kéki, Sándor
Encoding Information into Polyethylene Glycol Using an Alcohol-Isocyanate “Click” Reaction
title Encoding Information into Polyethylene Glycol Using an Alcohol-Isocyanate “Click” Reaction
title_full Encoding Information into Polyethylene Glycol Using an Alcohol-Isocyanate “Click” Reaction
title_fullStr Encoding Information into Polyethylene Glycol Using an Alcohol-Isocyanate “Click” Reaction
title_full_unstemmed Encoding Information into Polyethylene Glycol Using an Alcohol-Isocyanate “Click” Reaction
title_short Encoding Information into Polyethylene Glycol Using an Alcohol-Isocyanate “Click” Reaction
title_sort encoding information into polyethylene glycol using an alcohol-isocyanate “click” reaction
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7072859/
https://www.ncbi.nlm.nih.gov/pubmed/32075293
http://dx.doi.org/10.3390/ijms21041318
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