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Characterization of the OFP Gene Family and its Putative Involvement of Tuberous Root Shape in Radish

The shape of the tuberous root, a very important quality trait, varies dramatically among radish cultivars. Ovate family proteins (OFPs) are plant-specific proteins that regulate multiple aspects of plant growth and development. To investigate the possible role of OFPs in radish tuberous root format...

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Autores principales: Wang, Yanping, Wang, Qingbiao, Hao, Wei, Sun, Honghe, Zhang, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7072887/
https://www.ncbi.nlm.nih.gov/pubmed/32075122
http://dx.doi.org/10.3390/ijms21041293
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author Wang, Yanping
Wang, Qingbiao
Hao, Wei
Sun, Honghe
Zhang, Li
author_facet Wang, Yanping
Wang, Qingbiao
Hao, Wei
Sun, Honghe
Zhang, Li
author_sort Wang, Yanping
collection PubMed
description The shape of the tuberous root, a very important quality trait, varies dramatically among radish cultivars. Ovate family proteins (OFPs) are plant-specific proteins that regulate multiple aspects of plant growth and development. To investigate the possible role of OFPs in radish tuberous root formation, 35 putative RsOFPs were identified from radish, and their expression patterns were detected during tuberous root development in six different radish cultivars. Phylogenetically, RsOFP2.3 clustered together with AtOFP1 and other members of this family that are known to regulate organ shape. Moreover, RsOFP2.3 expression was negatively correlated with tuberous root elongation after the cortex splitting stage, which made this gene the top candidate for the involvement of tuberous root shape. To further characterize the function of RsOFP2.3, it was ectopically expressed in Arabidopsis. RsOFP2.3 overexpression in Arabidopsis led to multiple phenotypical changes, especially the decreased length and increased width of the hypocotyl. Furthermore, RsOFP2.3 expression was induced by all the five classic plant hormones except ethylene, and it was most sensitive to exogenous gibberellic acid treatment. We also found that RsOFP2.3 was localized in the cytoplasm. Taken together, our results suggested the possible involvement for RsOFP2.3 in suppressing radish tuberous root elongation and that it encodes a functional protein which mainly inhibits the elongation of Arabidopsis aerial organs.
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spelling pubmed-70728872020-03-19 Characterization of the OFP Gene Family and its Putative Involvement of Tuberous Root Shape in Radish Wang, Yanping Wang, Qingbiao Hao, Wei Sun, Honghe Zhang, Li Int J Mol Sci Article The shape of the tuberous root, a very important quality trait, varies dramatically among radish cultivars. Ovate family proteins (OFPs) are plant-specific proteins that regulate multiple aspects of plant growth and development. To investigate the possible role of OFPs in radish tuberous root formation, 35 putative RsOFPs were identified from radish, and their expression patterns were detected during tuberous root development in six different radish cultivars. Phylogenetically, RsOFP2.3 clustered together with AtOFP1 and other members of this family that are known to regulate organ shape. Moreover, RsOFP2.3 expression was negatively correlated with tuberous root elongation after the cortex splitting stage, which made this gene the top candidate for the involvement of tuberous root shape. To further characterize the function of RsOFP2.3, it was ectopically expressed in Arabidopsis. RsOFP2.3 overexpression in Arabidopsis led to multiple phenotypical changes, especially the decreased length and increased width of the hypocotyl. Furthermore, RsOFP2.3 expression was induced by all the five classic plant hormones except ethylene, and it was most sensitive to exogenous gibberellic acid treatment. We also found that RsOFP2.3 was localized in the cytoplasm. Taken together, our results suggested the possible involvement for RsOFP2.3 in suppressing radish tuberous root elongation and that it encodes a functional protein which mainly inhibits the elongation of Arabidopsis aerial organs. MDPI 2020-02-14 /pmc/articles/PMC7072887/ /pubmed/32075122 http://dx.doi.org/10.3390/ijms21041293 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Yanping
Wang, Qingbiao
Hao, Wei
Sun, Honghe
Zhang, Li
Characterization of the OFP Gene Family and its Putative Involvement of Tuberous Root Shape in Radish
title Characterization of the OFP Gene Family and its Putative Involvement of Tuberous Root Shape in Radish
title_full Characterization of the OFP Gene Family and its Putative Involvement of Tuberous Root Shape in Radish
title_fullStr Characterization of the OFP Gene Family and its Putative Involvement of Tuberous Root Shape in Radish
title_full_unstemmed Characterization of the OFP Gene Family and its Putative Involvement of Tuberous Root Shape in Radish
title_short Characterization of the OFP Gene Family and its Putative Involvement of Tuberous Root Shape in Radish
title_sort characterization of the ofp gene family and its putative involvement of tuberous root shape in radish
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7072887/
https://www.ncbi.nlm.nih.gov/pubmed/32075122
http://dx.doi.org/10.3390/ijms21041293
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