Cargando…

mRNA with Mammalian Codon Bias Accumulates in Yeast Mutants with Constitutive Stress Granules

Stress granules and P bodies are cytoplasmic structures assembled in response to various stress factors and represent sites of temporary storage or decay of mRNAs. Depending on the source of stress, the formation of these structures may be driven by distinct mechanisms, but several stresses have bee...

Descripción completa

Detalles Bibliográficos
Autores principales: Kozlova, Natalia V., Pichon, Chantal, Rahmouni, A. Rachid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7072924/
https://www.ncbi.nlm.nih.gov/pubmed/32059599
http://dx.doi.org/10.3390/ijms21041234
_version_ 1783506519674847232
author Kozlova, Natalia V.
Pichon, Chantal
Rahmouni, A. Rachid
author_facet Kozlova, Natalia V.
Pichon, Chantal
Rahmouni, A. Rachid
author_sort Kozlova, Natalia V.
collection PubMed
description Stress granules and P bodies are cytoplasmic structures assembled in response to various stress factors and represent sites of temporary storage or decay of mRNAs. Depending on the source of stress, the formation of these structures may be driven by distinct mechanisms, but several stresses have been shown to stabilize mRNAs via inhibition of deadenylation. A recent study identified yeast gene deletion mutants with constitutive stress granules and elevated P bodies; however, the mechanisms which trigger its formation remain poorly understood. Here, we investigate the possibility of accumulating mRNA with mammalian codon bias, which we termed the model RNA, in these mutants. We found that the model RNA accumulates in dcp2 and xrn1 mutants and in four mutants with constitutive stress granules overlapping with P bodies. However, in eight other mutants with constitutive stress granules, the model RNA is downregulated, or its steady state levels vary. We further suggest that the accumulation of the model RNA is linked to its protection from the main mRNA surveillance path. However, there is no obvious targeting of the model RNA to stress granules or P bodies. Thus, accumulation of the model RNA and formation of constitutive stress granules occur independently and only some paths inducing formation of constitutive stress granules will stabilize mRNA as well.
format Online
Article
Text
id pubmed-7072924
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-70729242020-03-19 mRNA with Mammalian Codon Bias Accumulates in Yeast Mutants with Constitutive Stress Granules Kozlova, Natalia V. Pichon, Chantal Rahmouni, A. Rachid Int J Mol Sci Article Stress granules and P bodies are cytoplasmic structures assembled in response to various stress factors and represent sites of temporary storage or decay of mRNAs. Depending on the source of stress, the formation of these structures may be driven by distinct mechanisms, but several stresses have been shown to stabilize mRNAs via inhibition of deadenylation. A recent study identified yeast gene deletion mutants with constitutive stress granules and elevated P bodies; however, the mechanisms which trigger its formation remain poorly understood. Here, we investigate the possibility of accumulating mRNA with mammalian codon bias, which we termed the model RNA, in these mutants. We found that the model RNA accumulates in dcp2 and xrn1 mutants and in four mutants with constitutive stress granules overlapping with P bodies. However, in eight other mutants with constitutive stress granules, the model RNA is downregulated, or its steady state levels vary. We further suggest that the accumulation of the model RNA is linked to its protection from the main mRNA surveillance path. However, there is no obvious targeting of the model RNA to stress granules or P bodies. Thus, accumulation of the model RNA and formation of constitutive stress granules occur independently and only some paths inducing formation of constitutive stress granules will stabilize mRNA as well. MDPI 2020-02-12 /pmc/articles/PMC7072924/ /pubmed/32059599 http://dx.doi.org/10.3390/ijms21041234 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kozlova, Natalia V.
Pichon, Chantal
Rahmouni, A. Rachid
mRNA with Mammalian Codon Bias Accumulates in Yeast Mutants with Constitutive Stress Granules
title mRNA with Mammalian Codon Bias Accumulates in Yeast Mutants with Constitutive Stress Granules
title_full mRNA with Mammalian Codon Bias Accumulates in Yeast Mutants with Constitutive Stress Granules
title_fullStr mRNA with Mammalian Codon Bias Accumulates in Yeast Mutants with Constitutive Stress Granules
title_full_unstemmed mRNA with Mammalian Codon Bias Accumulates in Yeast Mutants with Constitutive Stress Granules
title_short mRNA with Mammalian Codon Bias Accumulates in Yeast Mutants with Constitutive Stress Granules
title_sort mrna with mammalian codon bias accumulates in yeast mutants with constitutive stress granules
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7072924/
https://www.ncbi.nlm.nih.gov/pubmed/32059599
http://dx.doi.org/10.3390/ijms21041234
work_keys_str_mv AT kozlovanataliav mrnawithmammaliancodonbiasaccumulatesinyeastmutantswithconstitutivestressgranules
AT pichonchantal mrnawithmammaliancodonbiasaccumulatesinyeastmutantswithconstitutivestressgranules
AT rahmouniarachid mrnawithmammaliancodonbiasaccumulatesinyeastmutantswithconstitutivestressgranules