Cargando…

Cbx2, a PcG Family Gene, Plays a Regulatory Role in Medaka Gonadal Development

Chromobox homolog 2 (CBX2), a key member of the polycomb group (PcG) family, is essential for gonadal development in mammals. A functional deficiency or genetic mutation in cbx2 can lead to sex reversal in mice and humans. However, little is known about the function of cbx2 in gonadal development in...

Descripción completa

Detalles Bibliográficos
Autores principales: Chao, Qinghe, Shen, Fengfeng, Xue, Yidong, Wu, Jikui, Zhang, Junling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7072964/
https://www.ncbi.nlm.nih.gov/pubmed/32075028
http://dx.doi.org/10.3390/ijms21041288
_version_ 1783506529060651008
author Chao, Qinghe
Shen, Fengfeng
Xue, Yidong
Wu, Jikui
Zhang, Junling
author_facet Chao, Qinghe
Shen, Fengfeng
Xue, Yidong
Wu, Jikui
Zhang, Junling
author_sort Chao, Qinghe
collection PubMed
description Chromobox homolog 2 (CBX2), a key member of the polycomb group (PcG) family, is essential for gonadal development in mammals. A functional deficiency or genetic mutation in cbx2 can lead to sex reversal in mice and humans. However, little is known about the function of cbx2 in gonadal development in fish. In this study, the cbx2 gene was identified in medaka, which is a model species for the study of gonadal development in fish. Transcription of cbx2 was abundant in the gonads, with testicular levels relatively higher than ovarian levels. In situ hybridization (ISH) revealed that cbx2 mRNA was predominately localized in spermatogonia and spermatocytes, and was also observed in oocytes at stages I, II, and III. Furthermore, cbx2 and vasa (a marker gene) were co-localized in germ cells by fluorescent in situ hybridization (FISH). After cbx2 knockdown in the gonads by RNA interference (RNAi), the sex-related genes, including sox9 and foxl2, were influenced. These results suggest that cbx2 not only plays a positive role in spermatogenesis and oogenesis but is also involved in gonadal differentiation through regulating the expression levels of sex-related genes in fish.
format Online
Article
Text
id pubmed-7072964
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-70729642020-03-19 Cbx2, a PcG Family Gene, Plays a Regulatory Role in Medaka Gonadal Development Chao, Qinghe Shen, Fengfeng Xue, Yidong Wu, Jikui Zhang, Junling Int J Mol Sci Article Chromobox homolog 2 (CBX2), a key member of the polycomb group (PcG) family, is essential for gonadal development in mammals. A functional deficiency or genetic mutation in cbx2 can lead to sex reversal in mice and humans. However, little is known about the function of cbx2 in gonadal development in fish. In this study, the cbx2 gene was identified in medaka, which is a model species for the study of gonadal development in fish. Transcription of cbx2 was abundant in the gonads, with testicular levels relatively higher than ovarian levels. In situ hybridization (ISH) revealed that cbx2 mRNA was predominately localized in spermatogonia and spermatocytes, and was also observed in oocytes at stages I, II, and III. Furthermore, cbx2 and vasa (a marker gene) were co-localized in germ cells by fluorescent in situ hybridization (FISH). After cbx2 knockdown in the gonads by RNA interference (RNAi), the sex-related genes, including sox9 and foxl2, were influenced. These results suggest that cbx2 not only plays a positive role in spermatogenesis and oogenesis but is also involved in gonadal differentiation through regulating the expression levels of sex-related genes in fish. MDPI 2020-02-14 /pmc/articles/PMC7072964/ /pubmed/32075028 http://dx.doi.org/10.3390/ijms21041288 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chao, Qinghe
Shen, Fengfeng
Xue, Yidong
Wu, Jikui
Zhang, Junling
Cbx2, a PcG Family Gene, Plays a Regulatory Role in Medaka Gonadal Development
title Cbx2, a PcG Family Gene, Plays a Regulatory Role in Medaka Gonadal Development
title_full Cbx2, a PcG Family Gene, Plays a Regulatory Role in Medaka Gonadal Development
title_fullStr Cbx2, a PcG Family Gene, Plays a Regulatory Role in Medaka Gonadal Development
title_full_unstemmed Cbx2, a PcG Family Gene, Plays a Regulatory Role in Medaka Gonadal Development
title_short Cbx2, a PcG Family Gene, Plays a Regulatory Role in Medaka Gonadal Development
title_sort cbx2, a pcg family gene, plays a regulatory role in medaka gonadal development
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7072964/
https://www.ncbi.nlm.nih.gov/pubmed/32075028
http://dx.doi.org/10.3390/ijms21041288
work_keys_str_mv AT chaoqinghe cbx2apcgfamilygeneplaysaregulatoryroleinmedakagonadaldevelopment
AT shenfengfeng cbx2apcgfamilygeneplaysaregulatoryroleinmedakagonadaldevelopment
AT xueyidong cbx2apcgfamilygeneplaysaregulatoryroleinmedakagonadaldevelopment
AT wujikui cbx2apcgfamilygeneplaysaregulatoryroleinmedakagonadaldevelopment
AT zhangjunling cbx2apcgfamilygeneplaysaregulatoryroleinmedakagonadaldevelopment