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Cbx2, a PcG Family Gene, Plays a Regulatory Role in Medaka Gonadal Development
Chromobox homolog 2 (CBX2), a key member of the polycomb group (PcG) family, is essential for gonadal development in mammals. A functional deficiency or genetic mutation in cbx2 can lead to sex reversal in mice and humans. However, little is known about the function of cbx2 in gonadal development in...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7072964/ https://www.ncbi.nlm.nih.gov/pubmed/32075028 http://dx.doi.org/10.3390/ijms21041288 |
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author | Chao, Qinghe Shen, Fengfeng Xue, Yidong Wu, Jikui Zhang, Junling |
author_facet | Chao, Qinghe Shen, Fengfeng Xue, Yidong Wu, Jikui Zhang, Junling |
author_sort | Chao, Qinghe |
collection | PubMed |
description | Chromobox homolog 2 (CBX2), a key member of the polycomb group (PcG) family, is essential for gonadal development in mammals. A functional deficiency or genetic mutation in cbx2 can lead to sex reversal in mice and humans. However, little is known about the function of cbx2 in gonadal development in fish. In this study, the cbx2 gene was identified in medaka, which is a model species for the study of gonadal development in fish. Transcription of cbx2 was abundant in the gonads, with testicular levels relatively higher than ovarian levels. In situ hybridization (ISH) revealed that cbx2 mRNA was predominately localized in spermatogonia and spermatocytes, and was also observed in oocytes at stages I, II, and III. Furthermore, cbx2 and vasa (a marker gene) were co-localized in germ cells by fluorescent in situ hybridization (FISH). After cbx2 knockdown in the gonads by RNA interference (RNAi), the sex-related genes, including sox9 and foxl2, were influenced. These results suggest that cbx2 not only plays a positive role in spermatogenesis and oogenesis but is also involved in gonadal differentiation through regulating the expression levels of sex-related genes in fish. |
format | Online Article Text |
id | pubmed-7072964 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-70729642020-03-19 Cbx2, a PcG Family Gene, Plays a Regulatory Role in Medaka Gonadal Development Chao, Qinghe Shen, Fengfeng Xue, Yidong Wu, Jikui Zhang, Junling Int J Mol Sci Article Chromobox homolog 2 (CBX2), a key member of the polycomb group (PcG) family, is essential for gonadal development in mammals. A functional deficiency or genetic mutation in cbx2 can lead to sex reversal in mice and humans. However, little is known about the function of cbx2 in gonadal development in fish. In this study, the cbx2 gene was identified in medaka, which is a model species for the study of gonadal development in fish. Transcription of cbx2 was abundant in the gonads, with testicular levels relatively higher than ovarian levels. In situ hybridization (ISH) revealed that cbx2 mRNA was predominately localized in spermatogonia and spermatocytes, and was also observed in oocytes at stages I, II, and III. Furthermore, cbx2 and vasa (a marker gene) were co-localized in germ cells by fluorescent in situ hybridization (FISH). After cbx2 knockdown in the gonads by RNA interference (RNAi), the sex-related genes, including sox9 and foxl2, were influenced. These results suggest that cbx2 not only plays a positive role in spermatogenesis and oogenesis but is also involved in gonadal differentiation through regulating the expression levels of sex-related genes in fish. MDPI 2020-02-14 /pmc/articles/PMC7072964/ /pubmed/32075028 http://dx.doi.org/10.3390/ijms21041288 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Chao, Qinghe Shen, Fengfeng Xue, Yidong Wu, Jikui Zhang, Junling Cbx2, a PcG Family Gene, Plays a Regulatory Role in Medaka Gonadal Development |
title | Cbx2, a PcG Family Gene, Plays a Regulatory Role in Medaka Gonadal Development |
title_full | Cbx2, a PcG Family Gene, Plays a Regulatory Role in Medaka Gonadal Development |
title_fullStr | Cbx2, a PcG Family Gene, Plays a Regulatory Role in Medaka Gonadal Development |
title_full_unstemmed | Cbx2, a PcG Family Gene, Plays a Regulatory Role in Medaka Gonadal Development |
title_short | Cbx2, a PcG Family Gene, Plays a Regulatory Role in Medaka Gonadal Development |
title_sort | cbx2, a pcg family gene, plays a regulatory role in medaka gonadal development |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7072964/ https://www.ncbi.nlm.nih.gov/pubmed/32075028 http://dx.doi.org/10.3390/ijms21041288 |
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