Identifying the Translatome of Mouse NEBD-Stage Oocytes via SSP-Profiling; A Novel Polysome Fractionation Method

Meiotic maturation of oocyte relies on pre-synthesised maternal mRNA, the translation of which is highly coordinated in space and time. Here, we provide a detailed polysome profiling protocol that demonstrates a combination of the sucrose gradient ultracentrifugation in small SW55Ti tubes with the q...

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Detalles Bibliográficos
Autores principales: Masek, Tomas, del Llano, Edgar, Gahurova, Lenka, Kubelka, Michal, Susor, Andrej, Roucova, Kristina, Lin, Chih-Jen, Bruce, Alexander W., Pospisek, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7072993/
https://www.ncbi.nlm.nih.gov/pubmed/32070012
http://dx.doi.org/10.3390/ijms21041254

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