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miR-34a regulates adipogenesis in porcine intramuscular adipocytes by targeting ACSL4
BACKGROUND: Intramuscular fat (IMF) content is an important factor in porcine meat quality. Previously, we showed that miR-34a was less abundant in liver tissue from pigs with higher backfat thickness, compared to pigs with lower backfat thickness. The purpose of this present study was to explore th...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7073017/ https://www.ncbi.nlm.nih.gov/pubmed/32171241 http://dx.doi.org/10.1186/s12863-020-0836-7 |
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author | Wang, Wenwen Li, Xiuxiu Ding, Ning Teng, Jun Zhang, Shen Zhang, Qin Tang, Hui |
author_facet | Wang, Wenwen Li, Xiuxiu Ding, Ning Teng, Jun Zhang, Shen Zhang, Qin Tang, Hui |
author_sort | Wang, Wenwen |
collection | PubMed |
description | BACKGROUND: Intramuscular fat (IMF) content is an important factor in porcine meat quality. Previously, we showed that miR-34a was less abundant in liver tissue from pigs with higher backfat thickness, compared to pigs with lower backfat thickness. The purpose of this present study was to explore the role of miR-34a in adipogenesis. RESULT: Bioinformatics analysis identified Acyl-CoA synthetase long chain family member 4 (ACSL4) as a putative target of miR-34a. Using a luciferase reporter assay, we verified that miR-34a binds the ACSL4 mRNA at the 3’UTR. To examine the role of the miR-34a-ACSL4 interaction in IMF deposition in the pig, mRNA and protein expression of the ACSL4 gene was measured in primary intramuscular preadipocytes transfected with miR-34a mimic and inhibitor. Our results showed that ACSL4 is expressed throughout the entire differentiation process in pig preadipocytes, similar to the lipogenesis-associated genes PPARγ and aP2. Transfection with miR-34a mimic reduced lipid droplet formation during adipogenesis, while miR-34a inhibitor increased lipid droplet accumulation. Transfection with miR-34a mimic also reduced the mRNA and protein expression of ACSL4 and lipogenesis genes, including PPARγ, aP2, and SREBP-1C, but increased the expression of steatolysis genes such as ATGL and Sirt1. In contrast, the miR-34a inhibitor had the opposite effect on gene expression. Further, knockdown of ACSL4 decreased lipid droplet accumulation. CONCLUSIONS: Our results support the hypothesis that miR-34a regulates intramuscular fat deposition in porcine adipocytes by targeting ACSL4. |
format | Online Article Text |
id | pubmed-7073017 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-70730172020-03-18 miR-34a regulates adipogenesis in porcine intramuscular adipocytes by targeting ACSL4 Wang, Wenwen Li, Xiuxiu Ding, Ning Teng, Jun Zhang, Shen Zhang, Qin Tang, Hui BMC Genet Research Article BACKGROUND: Intramuscular fat (IMF) content is an important factor in porcine meat quality. Previously, we showed that miR-34a was less abundant in liver tissue from pigs with higher backfat thickness, compared to pigs with lower backfat thickness. The purpose of this present study was to explore the role of miR-34a in adipogenesis. RESULT: Bioinformatics analysis identified Acyl-CoA synthetase long chain family member 4 (ACSL4) as a putative target of miR-34a. Using a luciferase reporter assay, we verified that miR-34a binds the ACSL4 mRNA at the 3’UTR. To examine the role of the miR-34a-ACSL4 interaction in IMF deposition in the pig, mRNA and protein expression of the ACSL4 gene was measured in primary intramuscular preadipocytes transfected with miR-34a mimic and inhibitor. Our results showed that ACSL4 is expressed throughout the entire differentiation process in pig preadipocytes, similar to the lipogenesis-associated genes PPARγ and aP2. Transfection with miR-34a mimic reduced lipid droplet formation during adipogenesis, while miR-34a inhibitor increased lipid droplet accumulation. Transfection with miR-34a mimic also reduced the mRNA and protein expression of ACSL4 and lipogenesis genes, including PPARγ, aP2, and SREBP-1C, but increased the expression of steatolysis genes such as ATGL and Sirt1. In contrast, the miR-34a inhibitor had the opposite effect on gene expression. Further, knockdown of ACSL4 decreased lipid droplet accumulation. CONCLUSIONS: Our results support the hypothesis that miR-34a regulates intramuscular fat deposition in porcine adipocytes by targeting ACSL4. BioMed Central 2020-03-14 /pmc/articles/PMC7073017/ /pubmed/32171241 http://dx.doi.org/10.1186/s12863-020-0836-7 Text en © The Author(s). 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Wang, Wenwen Li, Xiuxiu Ding, Ning Teng, Jun Zhang, Shen Zhang, Qin Tang, Hui miR-34a regulates adipogenesis in porcine intramuscular adipocytes by targeting ACSL4 |
title | miR-34a regulates adipogenesis in porcine intramuscular adipocytes by targeting ACSL4 |
title_full | miR-34a regulates adipogenesis in porcine intramuscular adipocytes by targeting ACSL4 |
title_fullStr | miR-34a regulates adipogenesis in porcine intramuscular adipocytes by targeting ACSL4 |
title_full_unstemmed | miR-34a regulates adipogenesis in porcine intramuscular adipocytes by targeting ACSL4 |
title_short | miR-34a regulates adipogenesis in porcine intramuscular adipocytes by targeting ACSL4 |
title_sort | mir-34a regulates adipogenesis in porcine intramuscular adipocytes by targeting acsl4 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7073017/ https://www.ncbi.nlm.nih.gov/pubmed/32171241 http://dx.doi.org/10.1186/s12863-020-0836-7 |
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