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CLIC1 Protein Accumulates in Circulating Monocyte Membrane during Neurodegeneration

Pathologies that lead to neurodegeneration in the central nervous system (CNS) represent a major contemporary medical challenge. Neurodegenerative processes, like those that occur in Alzheimer’s disease (AD) are progressive, and at the moment, they are unstoppable. Not only is an adequate therapy mi...

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Autores principales: Carlini, Valentina, Verduci, Ivan, Cianci, Francesca, Cannavale, Gaetano, Fenoglio, Chiara, Galimberti, Daniela, Mazzanti, Michele
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7073046/
https://www.ncbi.nlm.nih.gov/pubmed/32098256
http://dx.doi.org/10.3390/ijms21041484
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author Carlini, Valentina
Verduci, Ivan
Cianci, Francesca
Cannavale, Gaetano
Fenoglio, Chiara
Galimberti, Daniela
Mazzanti, Michele
author_facet Carlini, Valentina
Verduci, Ivan
Cianci, Francesca
Cannavale, Gaetano
Fenoglio, Chiara
Galimberti, Daniela
Mazzanti, Michele
author_sort Carlini, Valentina
collection PubMed
description Pathologies that lead to neurodegeneration in the central nervous system (CNS) represent a major contemporary medical challenge. Neurodegenerative processes, like those that occur in Alzheimer’s disease (AD) are progressive, and at the moment, they are unstoppable. Not only is an adequate therapy missing but diagnosis is also extremely complicated. The most reliable method is the measurement of beta amyloid and tau peptides concentration in the cerebrospinal fluid (CSF). However, collecting liquid samples from the CNS is an invasive procedure, thus it is not suitable for a large-scale prevention program. Ideally, blood testing is the most manageable and appropriate diagnostic procedure for a massive population screening. Recently, a few candidates, including proteins or microRNAs present in plasma/serum have been identified. The aim of the present work is to propose the chloride intracellular channel 1 (CLIC1) protein as a potential marker of neurodegenerative processes. CLIC1 protein accumulates in peripheral blood mononuclear cells (PBMCs), and increases drastically when the CNS is in a chronic inflammatory state. In AD patients, both immunolocalization and mRNA quantification are able to show the behavior of CLIC1 during a persistent inflammatory state of the CNS. In particular, confocal microscopy analysis and electrophysiological measurements highlight the significant presence of transmembrane CLIC1 (tmCLIC1) in PBMCs from AD patients. Recent investigations suggest that tmCLIC1 has a very specific role. This provides an opportunity to use blood tests and conventional technologies to discriminate between healthy individuals and patients with ongoing neurodegenerative processes.
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spelling pubmed-70730462020-03-19 CLIC1 Protein Accumulates in Circulating Monocyte Membrane during Neurodegeneration Carlini, Valentina Verduci, Ivan Cianci, Francesca Cannavale, Gaetano Fenoglio, Chiara Galimberti, Daniela Mazzanti, Michele Int J Mol Sci Article Pathologies that lead to neurodegeneration in the central nervous system (CNS) represent a major contemporary medical challenge. Neurodegenerative processes, like those that occur in Alzheimer’s disease (AD) are progressive, and at the moment, they are unstoppable. Not only is an adequate therapy missing but diagnosis is also extremely complicated. The most reliable method is the measurement of beta amyloid and tau peptides concentration in the cerebrospinal fluid (CSF). However, collecting liquid samples from the CNS is an invasive procedure, thus it is not suitable for a large-scale prevention program. Ideally, blood testing is the most manageable and appropriate diagnostic procedure for a massive population screening. Recently, a few candidates, including proteins or microRNAs present in plasma/serum have been identified. The aim of the present work is to propose the chloride intracellular channel 1 (CLIC1) protein as a potential marker of neurodegenerative processes. CLIC1 protein accumulates in peripheral blood mononuclear cells (PBMCs), and increases drastically when the CNS is in a chronic inflammatory state. In AD patients, both immunolocalization and mRNA quantification are able to show the behavior of CLIC1 during a persistent inflammatory state of the CNS. In particular, confocal microscopy analysis and electrophysiological measurements highlight the significant presence of transmembrane CLIC1 (tmCLIC1) in PBMCs from AD patients. Recent investigations suggest that tmCLIC1 has a very specific role. This provides an opportunity to use blood tests and conventional technologies to discriminate between healthy individuals and patients with ongoing neurodegenerative processes. MDPI 2020-02-21 /pmc/articles/PMC7073046/ /pubmed/32098256 http://dx.doi.org/10.3390/ijms21041484 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Carlini, Valentina
Verduci, Ivan
Cianci, Francesca
Cannavale, Gaetano
Fenoglio, Chiara
Galimberti, Daniela
Mazzanti, Michele
CLIC1 Protein Accumulates in Circulating Monocyte Membrane during Neurodegeneration
title CLIC1 Protein Accumulates in Circulating Monocyte Membrane during Neurodegeneration
title_full CLIC1 Protein Accumulates in Circulating Monocyte Membrane during Neurodegeneration
title_fullStr CLIC1 Protein Accumulates in Circulating Monocyte Membrane during Neurodegeneration
title_full_unstemmed CLIC1 Protein Accumulates in Circulating Monocyte Membrane during Neurodegeneration
title_short CLIC1 Protein Accumulates in Circulating Monocyte Membrane during Neurodegeneration
title_sort clic1 protein accumulates in circulating monocyte membrane during neurodegeneration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7073046/
https://www.ncbi.nlm.nih.gov/pubmed/32098256
http://dx.doi.org/10.3390/ijms21041484
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