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Characterization of a Unique Bordetella bronchiseptica vB_BbrP_BB8 Bacteriophage and Its Application as an Antibacterial Agent

Bordetella bronchiseptica, an emerging zoonotic pathogen, infects a broad range of mammalian hosts. B. bronchiseptica-associated atrophic rhinitis incurs substantial losses to the pig breeding industry. The true burden of human disease caused by B. bronchiseptica is unknown, but it has been postulat...

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Autores principales: Szymczak, Mateusz, Grygorcewicz, Bartłomiej, Karczewska-Golec, Joanna, Decewicz, Przemysław, Pankowski, Jarosław Adam, Országh-Szturo, Hanna, Bącal, Paweł, Dołęgowska, Barbara, Golec, Piotr
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7073063/
https://www.ncbi.nlm.nih.gov/pubmed/32093105
http://dx.doi.org/10.3390/ijms21041403
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author Szymczak, Mateusz
Grygorcewicz, Bartłomiej
Karczewska-Golec, Joanna
Decewicz, Przemysław
Pankowski, Jarosław Adam
Országh-Szturo, Hanna
Bącal, Paweł
Dołęgowska, Barbara
Golec, Piotr
author_facet Szymczak, Mateusz
Grygorcewicz, Bartłomiej
Karczewska-Golec, Joanna
Decewicz, Przemysław
Pankowski, Jarosław Adam
Országh-Szturo, Hanna
Bącal, Paweł
Dołęgowska, Barbara
Golec, Piotr
author_sort Szymczak, Mateusz
collection PubMed
description Bordetella bronchiseptica, an emerging zoonotic pathogen, infects a broad range of mammalian hosts. B. bronchiseptica-associated atrophic rhinitis incurs substantial losses to the pig breeding industry. The true burden of human disease caused by B. bronchiseptica is unknown, but it has been postulated that some hypervirulent B. bronchiseptica isolates may be responsible for undiagnosed respiratory infections in humans. B. bronchiseptica was shown to acquire antibiotic resistance genes from other bacterial genera, especially Escherichia coli. Here, we present a new B. bronchiseptica lytic bacteriophage—vB_BbrP_BB8—of the Podoviridae family, which offers a safe alternative to antibiotic treatment of B. bronchiseptica infections. We explored the phage at the level of genome, physiology, morphology, and infection kinetics. Its therapeutic potential was investigated in biofilms and in an in vivo Galleria mellonella model, both of which mimic the natural environment of infection. The BB8 is a unique phage with a genome structure resembling that of T7-like phages. Its latent period is 75 ± 5 min and its burst size is 88 ± 10 phages. The BB8 infection causes complete lysis of B. bronchiseptica cultures irrespective of the MOI used. The phage efficiently removes bacterial biofilm and prevents the lethality induced by B. bronchiseptica in G. mellonella honeycomb moth larvae.
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spelling pubmed-70730632020-03-19 Characterization of a Unique Bordetella bronchiseptica vB_BbrP_BB8 Bacteriophage and Its Application as an Antibacterial Agent Szymczak, Mateusz Grygorcewicz, Bartłomiej Karczewska-Golec, Joanna Decewicz, Przemysław Pankowski, Jarosław Adam Országh-Szturo, Hanna Bącal, Paweł Dołęgowska, Barbara Golec, Piotr Int J Mol Sci Article Bordetella bronchiseptica, an emerging zoonotic pathogen, infects a broad range of mammalian hosts. B. bronchiseptica-associated atrophic rhinitis incurs substantial losses to the pig breeding industry. The true burden of human disease caused by B. bronchiseptica is unknown, but it has been postulated that some hypervirulent B. bronchiseptica isolates may be responsible for undiagnosed respiratory infections in humans. B. bronchiseptica was shown to acquire antibiotic resistance genes from other bacterial genera, especially Escherichia coli. Here, we present a new B. bronchiseptica lytic bacteriophage—vB_BbrP_BB8—of the Podoviridae family, which offers a safe alternative to antibiotic treatment of B. bronchiseptica infections. We explored the phage at the level of genome, physiology, morphology, and infection kinetics. Its therapeutic potential was investigated in biofilms and in an in vivo Galleria mellonella model, both of which mimic the natural environment of infection. The BB8 is a unique phage with a genome structure resembling that of T7-like phages. Its latent period is 75 ± 5 min and its burst size is 88 ± 10 phages. The BB8 infection causes complete lysis of B. bronchiseptica cultures irrespective of the MOI used. The phage efficiently removes bacterial biofilm and prevents the lethality induced by B. bronchiseptica in G. mellonella honeycomb moth larvae. MDPI 2020-02-19 /pmc/articles/PMC7073063/ /pubmed/32093105 http://dx.doi.org/10.3390/ijms21041403 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Szymczak, Mateusz
Grygorcewicz, Bartłomiej
Karczewska-Golec, Joanna
Decewicz, Przemysław
Pankowski, Jarosław Adam
Országh-Szturo, Hanna
Bącal, Paweł
Dołęgowska, Barbara
Golec, Piotr
Characterization of a Unique Bordetella bronchiseptica vB_BbrP_BB8 Bacteriophage and Its Application as an Antibacterial Agent
title Characterization of a Unique Bordetella bronchiseptica vB_BbrP_BB8 Bacteriophage and Its Application as an Antibacterial Agent
title_full Characterization of a Unique Bordetella bronchiseptica vB_BbrP_BB8 Bacteriophage and Its Application as an Antibacterial Agent
title_fullStr Characterization of a Unique Bordetella bronchiseptica vB_BbrP_BB8 Bacteriophage and Its Application as an Antibacterial Agent
title_full_unstemmed Characterization of a Unique Bordetella bronchiseptica vB_BbrP_BB8 Bacteriophage and Its Application as an Antibacterial Agent
title_short Characterization of a Unique Bordetella bronchiseptica vB_BbrP_BB8 Bacteriophage and Its Application as an Antibacterial Agent
title_sort characterization of a unique bordetella bronchiseptica vb_bbrp_bb8 bacteriophage and its application as an antibacterial agent
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7073063/
https://www.ncbi.nlm.nih.gov/pubmed/32093105
http://dx.doi.org/10.3390/ijms21041403
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