Cargando…

Interpenetrating Hydrogel Networks Enhance Mechanical Stability, Rheological Properties, Release Behavior and Adhesiveness of Platelet-Rich Plasma

Platelet-rich plasma (PRP) has attracted much attention for the treatment of articular cartilage defects or wounds due to its intrinsic content of growth factors relevant for tissue repair. However, the short residence time of PRP in vivo, due to the action of lytic enzymes, its weak mechanical prop...

Descripción completa

Detalles Bibliográficos
Autores principales: Censi, Roberta, Casadidio, Cristina, Deng, Siyuan, Gigliobianco, Maria Rosa, Sabbieti, Maria Giovanna, Agas, Dimitrios, Laus, Fulvio, Di Martino, Piera
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7073123/
https://www.ncbi.nlm.nih.gov/pubmed/32092976
http://dx.doi.org/10.3390/ijms21041399
_version_ 1783506565344526336
author Censi, Roberta
Casadidio, Cristina
Deng, Siyuan
Gigliobianco, Maria Rosa
Sabbieti, Maria Giovanna
Agas, Dimitrios
Laus, Fulvio
Di Martino, Piera
author_facet Censi, Roberta
Casadidio, Cristina
Deng, Siyuan
Gigliobianco, Maria Rosa
Sabbieti, Maria Giovanna
Agas, Dimitrios
Laus, Fulvio
Di Martino, Piera
author_sort Censi, Roberta
collection PubMed
description Platelet-rich plasma (PRP) has attracted much attention for the treatment of articular cartilage defects or wounds due to its intrinsic content of growth factors relevant for tissue repair. However, the short residence time of PRP in vivo, due to the action of lytic enzymes, its weak mechanical properties and the consequent short-term release of bioactive factors has restricted its application and efficacy. The present work aimed at designing new formulation strategies for PRP, based on the use of platelet concentrate (PC)-loaded hydrogels or interpenetrating polymer networks, directed at improving mechanical stability and sustaining the release of bioactive growth factors over a prolonged time-span. The interpenetrating hydrogels comprised two polymer networks interlaced on a molecular scale: (a) a first covalent network of thermosensitive and biodegradable vinyl sulfone bearing p(hydroxypropyl methacrylamide-lacate)-polyethylene glycol triblock copolymers, tandem cross-linked by thermal gelation and Michael addition when combined with thiolated hyaluronic acid, and (b) a second network composed of cross-linked fibrin. The PC-loaded hydrogels, instead, was formed only by network (a). All the designed and successfully synthesized formulations greatly increased the stability of PRP in vitro, leading to significant increase in degradation time and storage modulus of PRP gel. The resulting viscoelastic networks showed the ability to controllably release platelet derived growth factor and transforming growth factr β1, and to improve the tissue adhesiveness of PRP. The newly developed hydrogels show great potential for application in the field of wound healing, cartilage repair and beyond.
format Online
Article
Text
id pubmed-7073123
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-70731232020-03-19 Interpenetrating Hydrogel Networks Enhance Mechanical Stability, Rheological Properties, Release Behavior and Adhesiveness of Platelet-Rich Plasma Censi, Roberta Casadidio, Cristina Deng, Siyuan Gigliobianco, Maria Rosa Sabbieti, Maria Giovanna Agas, Dimitrios Laus, Fulvio Di Martino, Piera Int J Mol Sci Article Platelet-rich plasma (PRP) has attracted much attention for the treatment of articular cartilage defects or wounds due to its intrinsic content of growth factors relevant for tissue repair. However, the short residence time of PRP in vivo, due to the action of lytic enzymes, its weak mechanical properties and the consequent short-term release of bioactive factors has restricted its application and efficacy. The present work aimed at designing new formulation strategies for PRP, based on the use of platelet concentrate (PC)-loaded hydrogels or interpenetrating polymer networks, directed at improving mechanical stability and sustaining the release of bioactive growth factors over a prolonged time-span. The interpenetrating hydrogels comprised two polymer networks interlaced on a molecular scale: (a) a first covalent network of thermosensitive and biodegradable vinyl sulfone bearing p(hydroxypropyl methacrylamide-lacate)-polyethylene glycol triblock copolymers, tandem cross-linked by thermal gelation and Michael addition when combined with thiolated hyaluronic acid, and (b) a second network composed of cross-linked fibrin. The PC-loaded hydrogels, instead, was formed only by network (a). All the designed and successfully synthesized formulations greatly increased the stability of PRP in vitro, leading to significant increase in degradation time and storage modulus of PRP gel. The resulting viscoelastic networks showed the ability to controllably release platelet derived growth factor and transforming growth factr β1, and to improve the tissue adhesiveness of PRP. The newly developed hydrogels show great potential for application in the field of wound healing, cartilage repair and beyond. MDPI 2020-02-19 /pmc/articles/PMC7073123/ /pubmed/32092976 http://dx.doi.org/10.3390/ijms21041399 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Censi, Roberta
Casadidio, Cristina
Deng, Siyuan
Gigliobianco, Maria Rosa
Sabbieti, Maria Giovanna
Agas, Dimitrios
Laus, Fulvio
Di Martino, Piera
Interpenetrating Hydrogel Networks Enhance Mechanical Stability, Rheological Properties, Release Behavior and Adhesiveness of Platelet-Rich Plasma
title Interpenetrating Hydrogel Networks Enhance Mechanical Stability, Rheological Properties, Release Behavior and Adhesiveness of Platelet-Rich Plasma
title_full Interpenetrating Hydrogel Networks Enhance Mechanical Stability, Rheological Properties, Release Behavior and Adhesiveness of Platelet-Rich Plasma
title_fullStr Interpenetrating Hydrogel Networks Enhance Mechanical Stability, Rheological Properties, Release Behavior and Adhesiveness of Platelet-Rich Plasma
title_full_unstemmed Interpenetrating Hydrogel Networks Enhance Mechanical Stability, Rheological Properties, Release Behavior and Adhesiveness of Platelet-Rich Plasma
title_short Interpenetrating Hydrogel Networks Enhance Mechanical Stability, Rheological Properties, Release Behavior and Adhesiveness of Platelet-Rich Plasma
title_sort interpenetrating hydrogel networks enhance mechanical stability, rheological properties, release behavior and adhesiveness of platelet-rich plasma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7073123/
https://www.ncbi.nlm.nih.gov/pubmed/32092976
http://dx.doi.org/10.3390/ijms21041399
work_keys_str_mv AT censiroberta interpenetratinghydrogelnetworksenhancemechanicalstabilityrheologicalpropertiesreleasebehaviorandadhesivenessofplateletrichplasma
AT casadidiocristina interpenetratinghydrogelnetworksenhancemechanicalstabilityrheologicalpropertiesreleasebehaviorandadhesivenessofplateletrichplasma
AT dengsiyuan interpenetratinghydrogelnetworksenhancemechanicalstabilityrheologicalpropertiesreleasebehaviorandadhesivenessofplateletrichplasma
AT gigliobiancomariarosa interpenetratinghydrogelnetworksenhancemechanicalstabilityrheologicalpropertiesreleasebehaviorandadhesivenessofplateletrichplasma
AT sabbietimariagiovanna interpenetratinghydrogelnetworksenhancemechanicalstabilityrheologicalpropertiesreleasebehaviorandadhesivenessofplateletrichplasma
AT agasdimitrios interpenetratinghydrogelnetworksenhancemechanicalstabilityrheologicalpropertiesreleasebehaviorandadhesivenessofplateletrichplasma
AT lausfulvio interpenetratinghydrogelnetworksenhancemechanicalstabilityrheologicalpropertiesreleasebehaviorandadhesivenessofplateletrichplasma
AT dimartinopiera interpenetratinghydrogelnetworksenhancemechanicalstabilityrheologicalpropertiesreleasebehaviorandadhesivenessofplateletrichplasma