Cargando…

Characterization of a Novel Rice Dynamic Narrow-Rolled Leaf Mutant with Deficiencies in Aromatic Amino Acids

The leaf blade is the main photosynthetic organ and its morphology is related to light energy capture and conversion efficiency. We isolated a novel rice Dynamic Narrow-Rolled Leaf 1 (dnrl1) mutant showing reduced width of leaf blades, rolled leaves and lower chlorophyll content. The narrow-rolled l...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Huimei, Shi, Yongfeng, Zhang, Xiaobo, Xu, Xia, Wu, Jian-Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7073152/
https://www.ncbi.nlm.nih.gov/pubmed/32102218
http://dx.doi.org/10.3390/ijms21041521
_version_ 1783506571888689152
author Wang, Huimei
Shi, Yongfeng
Zhang, Xiaobo
Xu, Xia
Wu, Jian-Li
author_facet Wang, Huimei
Shi, Yongfeng
Zhang, Xiaobo
Xu, Xia
Wu, Jian-Li
author_sort Wang, Huimei
collection PubMed
description The leaf blade is the main photosynthetic organ and its morphology is related to light energy capture and conversion efficiency. We isolated a novel rice Dynamic Narrow-Rolled Leaf 1 (dnrl1) mutant showing reduced width of leaf blades, rolled leaves and lower chlorophyll content. The narrow-rolled leaf phenotype resulted from the reduced number of small longitudinal veins per leaf, smaller size and irregular arrangement of bulliform cells compared with the wild-type. DNRL1 was mapped to chromosome 7 and encoded a putative 3-deoxy-7-phosphoheptulonate synthase (DAHPS) which catalyzes the conversion of phosphoenolpyruvate and D-erythrose 4-phosphate to DAHP and phosphate. Sequence analysis revealed that a single base substitution (T–A) was detected in dnrl1, leading to a single amino acid change (L376H) in the coding protein. The mutation led to a lower expression level of DNRL1 as well as the lower activity of DAHPS in the mutant compared with the wild type. Genetic complementation and over-expression of DNRL1 could rescue the narrow-rolled phenotype. DNRL1 was constitutively expressed in all tested organs and exhibited different expression patterns from other narrow-rolled leaf genes. DNRL1-GFP located to chloroplasts. The lower level of chlorophyll in dnrl1 was associated with the downregulation of the genes responsible for chlorophyll biosynthesis and photosynthesis. Furthermore, dnrl1 showed significantly reduced levels of aromatic amino acids including Trp, Phe and Tyr. We conclude that OsDAHPS, encoded by DNRL1, plays a critical role in leaf morphogenesis by mediating the biosynthesis of amino acids in rice.
format Online
Article
Text
id pubmed-7073152
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-70731522020-03-19 Characterization of a Novel Rice Dynamic Narrow-Rolled Leaf Mutant with Deficiencies in Aromatic Amino Acids Wang, Huimei Shi, Yongfeng Zhang, Xiaobo Xu, Xia Wu, Jian-Li Int J Mol Sci Article The leaf blade is the main photosynthetic organ and its morphology is related to light energy capture and conversion efficiency. We isolated a novel rice Dynamic Narrow-Rolled Leaf 1 (dnrl1) mutant showing reduced width of leaf blades, rolled leaves and lower chlorophyll content. The narrow-rolled leaf phenotype resulted from the reduced number of small longitudinal veins per leaf, smaller size and irregular arrangement of bulliform cells compared with the wild-type. DNRL1 was mapped to chromosome 7 and encoded a putative 3-deoxy-7-phosphoheptulonate synthase (DAHPS) which catalyzes the conversion of phosphoenolpyruvate and D-erythrose 4-phosphate to DAHP and phosphate. Sequence analysis revealed that a single base substitution (T–A) was detected in dnrl1, leading to a single amino acid change (L376H) in the coding protein. The mutation led to a lower expression level of DNRL1 as well as the lower activity of DAHPS in the mutant compared with the wild type. Genetic complementation and over-expression of DNRL1 could rescue the narrow-rolled phenotype. DNRL1 was constitutively expressed in all tested organs and exhibited different expression patterns from other narrow-rolled leaf genes. DNRL1-GFP located to chloroplasts. The lower level of chlorophyll in dnrl1 was associated with the downregulation of the genes responsible for chlorophyll biosynthesis and photosynthesis. Furthermore, dnrl1 showed significantly reduced levels of aromatic amino acids including Trp, Phe and Tyr. We conclude that OsDAHPS, encoded by DNRL1, plays a critical role in leaf morphogenesis by mediating the biosynthesis of amino acids in rice. MDPI 2020-02-23 /pmc/articles/PMC7073152/ /pubmed/32102218 http://dx.doi.org/10.3390/ijms21041521 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Huimei
Shi, Yongfeng
Zhang, Xiaobo
Xu, Xia
Wu, Jian-Li
Characterization of a Novel Rice Dynamic Narrow-Rolled Leaf Mutant with Deficiencies in Aromatic Amino Acids
title Characterization of a Novel Rice Dynamic Narrow-Rolled Leaf Mutant with Deficiencies in Aromatic Amino Acids
title_full Characterization of a Novel Rice Dynamic Narrow-Rolled Leaf Mutant with Deficiencies in Aromatic Amino Acids
title_fullStr Characterization of a Novel Rice Dynamic Narrow-Rolled Leaf Mutant with Deficiencies in Aromatic Amino Acids
title_full_unstemmed Characterization of a Novel Rice Dynamic Narrow-Rolled Leaf Mutant with Deficiencies in Aromatic Amino Acids
title_short Characterization of a Novel Rice Dynamic Narrow-Rolled Leaf Mutant with Deficiencies in Aromatic Amino Acids
title_sort characterization of a novel rice dynamic narrow-rolled leaf mutant with deficiencies in aromatic amino acids
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7073152/
https://www.ncbi.nlm.nih.gov/pubmed/32102218
http://dx.doi.org/10.3390/ijms21041521
work_keys_str_mv AT wanghuimei characterizationofanovelricedynamicnarrowrolledleafmutantwithdeficienciesinaromaticaminoacids
AT shiyongfeng characterizationofanovelricedynamicnarrowrolledleafmutantwithdeficienciesinaromaticaminoacids
AT zhangxiaobo characterizationofanovelricedynamicnarrowrolledleafmutantwithdeficienciesinaromaticaminoacids
AT xuxia characterizationofanovelricedynamicnarrowrolledleafmutantwithdeficienciesinaromaticaminoacids
AT wujianli characterizationofanovelricedynamicnarrowrolledleafmutantwithdeficienciesinaromaticaminoacids