Cargando…

Surface Functionalization with Polyethylene Glycol and Polyethyleneimine Improves the Performance of Graphene-Based Materials for Safe and Efficient Intracellular Delivery by Laser-Induced Photoporation

Nanoparticle mediated laser-induced photoporation is a physical cell membrane disruption approach to directly deliver extrinsic molecules into living cells, which is particularly promising in applications for both adherent and suspension cells. In this work, we explored surface modifications of grap...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Jing, Li, Chengnan, Brans, Toon, Harizaj, Aranit, Van de Steene, Shana, De Beer, Thomas, De Smedt, Stefaan, Szunerits, Sabine, Boukherroub, Rabah, Xiong, Ranhua, Braeckmans, Kevin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7073198/
https://www.ncbi.nlm.nih.gov/pubmed/32102402
http://dx.doi.org/10.3390/ijms21041540
_version_ 1783506582692167680
author Liu, Jing
Li, Chengnan
Brans, Toon
Harizaj, Aranit
Van de Steene, Shana
De Beer, Thomas
De Smedt, Stefaan
Szunerits, Sabine
Boukherroub, Rabah
Xiong, Ranhua
Braeckmans, Kevin
author_facet Liu, Jing
Li, Chengnan
Brans, Toon
Harizaj, Aranit
Van de Steene, Shana
De Beer, Thomas
De Smedt, Stefaan
Szunerits, Sabine
Boukherroub, Rabah
Xiong, Ranhua
Braeckmans, Kevin
author_sort Liu, Jing
collection PubMed
description Nanoparticle mediated laser-induced photoporation is a physical cell membrane disruption approach to directly deliver extrinsic molecules into living cells, which is particularly promising in applications for both adherent and suspension cells. In this work, we explored surface modifications of graphene quantum dots (GQD) and reduced graphene oxide (rGO) with polyethylene glycol (PEG) and polyethyleneimine (PEI) to enhance colloidal stability while retaining photoporation functionality. After photoporation with FITC-dextran 10 kDa (FD10), the percentage of positive HeLa cells (81% for GQD-PEG, 74% for rGO-PEG and 90% for rGO-PEI) increased approximately two-fold compared to the bare nanomaterials. While for Jurkat suspension cells, the photoporation efficiency with polymer-modified graphene-based nanomaterial reached as high as 80%. Cell viability was >80% in all these cases. In addition, polymer functionalization proved to be beneficial for the delivery of larger macromolecules (FD70 and FD500) as well. Finally, we show that rGO is suitable for photoporation using a near-infrared laser to reach 80% FD10 positive HeLa cells at 80% cell viability. We conclude that modification of graphene-based nanoparticles with PEG and especially PEI provide better colloidal stability in cell medium, resulting in more uniform transfection and overall increased efficiency.
format Online
Article
Text
id pubmed-7073198
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-70731982020-03-19 Surface Functionalization with Polyethylene Glycol and Polyethyleneimine Improves the Performance of Graphene-Based Materials for Safe and Efficient Intracellular Delivery by Laser-Induced Photoporation Liu, Jing Li, Chengnan Brans, Toon Harizaj, Aranit Van de Steene, Shana De Beer, Thomas De Smedt, Stefaan Szunerits, Sabine Boukherroub, Rabah Xiong, Ranhua Braeckmans, Kevin Int J Mol Sci Article Nanoparticle mediated laser-induced photoporation is a physical cell membrane disruption approach to directly deliver extrinsic molecules into living cells, which is particularly promising in applications for both adherent and suspension cells. In this work, we explored surface modifications of graphene quantum dots (GQD) and reduced graphene oxide (rGO) with polyethylene glycol (PEG) and polyethyleneimine (PEI) to enhance colloidal stability while retaining photoporation functionality. After photoporation with FITC-dextran 10 kDa (FD10), the percentage of positive HeLa cells (81% for GQD-PEG, 74% for rGO-PEG and 90% for rGO-PEI) increased approximately two-fold compared to the bare nanomaterials. While for Jurkat suspension cells, the photoporation efficiency with polymer-modified graphene-based nanomaterial reached as high as 80%. Cell viability was >80% in all these cases. In addition, polymer functionalization proved to be beneficial for the delivery of larger macromolecules (FD70 and FD500) as well. Finally, we show that rGO is suitable for photoporation using a near-infrared laser to reach 80% FD10 positive HeLa cells at 80% cell viability. We conclude that modification of graphene-based nanoparticles with PEG and especially PEI provide better colloidal stability in cell medium, resulting in more uniform transfection and overall increased efficiency. MDPI 2020-02-24 /pmc/articles/PMC7073198/ /pubmed/32102402 http://dx.doi.org/10.3390/ijms21041540 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Jing
Li, Chengnan
Brans, Toon
Harizaj, Aranit
Van de Steene, Shana
De Beer, Thomas
De Smedt, Stefaan
Szunerits, Sabine
Boukherroub, Rabah
Xiong, Ranhua
Braeckmans, Kevin
Surface Functionalization with Polyethylene Glycol and Polyethyleneimine Improves the Performance of Graphene-Based Materials for Safe and Efficient Intracellular Delivery by Laser-Induced Photoporation
title Surface Functionalization with Polyethylene Glycol and Polyethyleneimine Improves the Performance of Graphene-Based Materials for Safe and Efficient Intracellular Delivery by Laser-Induced Photoporation
title_full Surface Functionalization with Polyethylene Glycol and Polyethyleneimine Improves the Performance of Graphene-Based Materials for Safe and Efficient Intracellular Delivery by Laser-Induced Photoporation
title_fullStr Surface Functionalization with Polyethylene Glycol and Polyethyleneimine Improves the Performance of Graphene-Based Materials for Safe and Efficient Intracellular Delivery by Laser-Induced Photoporation
title_full_unstemmed Surface Functionalization with Polyethylene Glycol and Polyethyleneimine Improves the Performance of Graphene-Based Materials for Safe and Efficient Intracellular Delivery by Laser-Induced Photoporation
title_short Surface Functionalization with Polyethylene Glycol and Polyethyleneimine Improves the Performance of Graphene-Based Materials for Safe and Efficient Intracellular Delivery by Laser-Induced Photoporation
title_sort surface functionalization with polyethylene glycol and polyethyleneimine improves the performance of graphene-based materials for safe and efficient intracellular delivery by laser-induced photoporation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7073198/
https://www.ncbi.nlm.nih.gov/pubmed/32102402
http://dx.doi.org/10.3390/ijms21041540
work_keys_str_mv AT liujing surfacefunctionalizationwithpolyethyleneglycolandpolyethyleneimineimprovestheperformanceofgraphenebasedmaterialsforsafeandefficientintracellulardeliverybylaserinducedphotoporation
AT lichengnan surfacefunctionalizationwithpolyethyleneglycolandpolyethyleneimineimprovestheperformanceofgraphenebasedmaterialsforsafeandefficientintracellulardeliverybylaserinducedphotoporation
AT branstoon surfacefunctionalizationwithpolyethyleneglycolandpolyethyleneimineimprovestheperformanceofgraphenebasedmaterialsforsafeandefficientintracellulardeliverybylaserinducedphotoporation
AT harizajaranit surfacefunctionalizationwithpolyethyleneglycolandpolyethyleneimineimprovestheperformanceofgraphenebasedmaterialsforsafeandefficientintracellulardeliverybylaserinducedphotoporation
AT vandesteeneshana surfacefunctionalizationwithpolyethyleneglycolandpolyethyleneimineimprovestheperformanceofgraphenebasedmaterialsforsafeandefficientintracellulardeliverybylaserinducedphotoporation
AT debeerthomas surfacefunctionalizationwithpolyethyleneglycolandpolyethyleneimineimprovestheperformanceofgraphenebasedmaterialsforsafeandefficientintracellulardeliverybylaserinducedphotoporation
AT desmedtstefaan surfacefunctionalizationwithpolyethyleneglycolandpolyethyleneimineimprovestheperformanceofgraphenebasedmaterialsforsafeandefficientintracellulardeliverybylaserinducedphotoporation
AT szuneritssabine surfacefunctionalizationwithpolyethyleneglycolandpolyethyleneimineimprovestheperformanceofgraphenebasedmaterialsforsafeandefficientintracellulardeliverybylaserinducedphotoporation
AT boukherroubrabah surfacefunctionalizationwithpolyethyleneglycolandpolyethyleneimineimprovestheperformanceofgraphenebasedmaterialsforsafeandefficientintracellulardeliverybylaserinducedphotoporation
AT xiongranhua surfacefunctionalizationwithpolyethyleneglycolandpolyethyleneimineimprovestheperformanceofgraphenebasedmaterialsforsafeandefficientintracellulardeliverybylaserinducedphotoporation
AT braeckmanskevin surfacefunctionalizationwithpolyethyleneglycolandpolyethyleneimineimprovestheperformanceofgraphenebasedmaterialsforsafeandefficientintracellulardeliverybylaserinducedphotoporation