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The Hypoxic Proteome and Metabolome of Barley (Hordeum vulgare L.) with and without Phytoglobin Priming

Overexpression of phytoglobins (formerly plant hemoglobins) increases the survival rate of plant tissues under hypoxia stress by the following two known mechanisms: (1) scavenging of nitric oxide (NO) in the phytoglobin/NO cycle and (2) mimicking ethylene priming to hypoxia when NO scavenging activa...

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Autores principales: Andrzejczak, Olga A., Havelund, Jesper F., Wang, Wei-Qing, Kovalchuk, Sergey, Hagensen, Christina E., Hasler-Sheetal, Harald, Jensen, Ole N., Rogowska-Wrzesinska, Adelina, Møller, Ian Max, Hebelstrup, Kim H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7073221/
https://www.ncbi.nlm.nih.gov/pubmed/32102473
http://dx.doi.org/10.3390/ijms21041546
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author Andrzejczak, Olga A.
Havelund, Jesper F.
Wang, Wei-Qing
Kovalchuk, Sergey
Hagensen, Christina E.
Hasler-Sheetal, Harald
Jensen, Ole N.
Rogowska-Wrzesinska, Adelina
Møller, Ian Max
Hebelstrup, Kim H.
author_facet Andrzejczak, Olga A.
Havelund, Jesper F.
Wang, Wei-Qing
Kovalchuk, Sergey
Hagensen, Christina E.
Hasler-Sheetal, Harald
Jensen, Ole N.
Rogowska-Wrzesinska, Adelina
Møller, Ian Max
Hebelstrup, Kim H.
author_sort Andrzejczak, Olga A.
collection PubMed
description Overexpression of phytoglobins (formerly plant hemoglobins) increases the survival rate of plant tissues under hypoxia stress by the following two known mechanisms: (1) scavenging of nitric oxide (NO) in the phytoglobin/NO cycle and (2) mimicking ethylene priming to hypoxia when NO scavenging activates transcription factors that are regulated by levels of NO and O(2) in the N-end rule pathway. To map the cellular and metabolic effects of hypoxia in barley (Hordeum vulgare L., cv. Golden Promise), with or without priming to hypoxia, we studied the proteome and metabolome of wild type (WT) and hemoglobin overexpressing (HO) plants in normoxia and after 24 h hypoxia (WT24, HO24). The WT plants were more susceptible to hypoxia than HO plants. The chlorophyll a + b content was lowered by 50% and biomass by 30% in WT24 compared to WT, while HO plants were unaffected. We observed an increase in ROS production during hypoxia treatment in WT seedlings that was not observed in HO seedlings. We identified and quantified 9694 proteins out of which 1107 changed significantly in abundance. Many proteins, such as ion transporters, Ca(2+)-signal transduction, and proteins related to protein degradation were downregulated in HO plants during hypoxia, but not in WT plants. Changes in the levels of histones indicates that chromatin restructuring plays a role in the priming of hypoxia. We also identified and quantified 1470 metabolites, of which the abundance of >500 changed significantly. In summary the data confirm known mechanisms of hypoxia priming by ethylene priming and N-end rule activation; however, the data also indicate the existence of other mechanisms for hypoxia priming in plants.
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spelling pubmed-70732212020-03-19 The Hypoxic Proteome and Metabolome of Barley (Hordeum vulgare L.) with and without Phytoglobin Priming Andrzejczak, Olga A. Havelund, Jesper F. Wang, Wei-Qing Kovalchuk, Sergey Hagensen, Christina E. Hasler-Sheetal, Harald Jensen, Ole N. Rogowska-Wrzesinska, Adelina Møller, Ian Max Hebelstrup, Kim H. Int J Mol Sci Article Overexpression of phytoglobins (formerly plant hemoglobins) increases the survival rate of plant tissues under hypoxia stress by the following two known mechanisms: (1) scavenging of nitric oxide (NO) in the phytoglobin/NO cycle and (2) mimicking ethylene priming to hypoxia when NO scavenging activates transcription factors that are regulated by levels of NO and O(2) in the N-end rule pathway. To map the cellular and metabolic effects of hypoxia in barley (Hordeum vulgare L., cv. Golden Promise), with or without priming to hypoxia, we studied the proteome and metabolome of wild type (WT) and hemoglobin overexpressing (HO) plants in normoxia and after 24 h hypoxia (WT24, HO24). The WT plants were more susceptible to hypoxia than HO plants. The chlorophyll a + b content was lowered by 50% and biomass by 30% in WT24 compared to WT, while HO plants were unaffected. We observed an increase in ROS production during hypoxia treatment in WT seedlings that was not observed in HO seedlings. We identified and quantified 9694 proteins out of which 1107 changed significantly in abundance. Many proteins, such as ion transporters, Ca(2+)-signal transduction, and proteins related to protein degradation were downregulated in HO plants during hypoxia, but not in WT plants. Changes in the levels of histones indicates that chromatin restructuring plays a role in the priming of hypoxia. We also identified and quantified 1470 metabolites, of which the abundance of >500 changed significantly. In summary the data confirm known mechanisms of hypoxia priming by ethylene priming and N-end rule activation; however, the data also indicate the existence of other mechanisms for hypoxia priming in plants. MDPI 2020-02-24 /pmc/articles/PMC7073221/ /pubmed/32102473 http://dx.doi.org/10.3390/ijms21041546 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Andrzejczak, Olga A.
Havelund, Jesper F.
Wang, Wei-Qing
Kovalchuk, Sergey
Hagensen, Christina E.
Hasler-Sheetal, Harald
Jensen, Ole N.
Rogowska-Wrzesinska, Adelina
Møller, Ian Max
Hebelstrup, Kim H.
The Hypoxic Proteome and Metabolome of Barley (Hordeum vulgare L.) with and without Phytoglobin Priming
title The Hypoxic Proteome and Metabolome of Barley (Hordeum vulgare L.) with and without Phytoglobin Priming
title_full The Hypoxic Proteome and Metabolome of Barley (Hordeum vulgare L.) with and without Phytoglobin Priming
title_fullStr The Hypoxic Proteome and Metabolome of Barley (Hordeum vulgare L.) with and without Phytoglobin Priming
title_full_unstemmed The Hypoxic Proteome and Metabolome of Barley (Hordeum vulgare L.) with and without Phytoglobin Priming
title_short The Hypoxic Proteome and Metabolome of Barley (Hordeum vulgare L.) with and without Phytoglobin Priming
title_sort hypoxic proteome and metabolome of barley (hordeum vulgare l.) with and without phytoglobin priming
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7073221/
https://www.ncbi.nlm.nih.gov/pubmed/32102473
http://dx.doi.org/10.3390/ijms21041546
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