Cargando…

Dynamics of the Transcriptome Response to Heat in the Moss, Physcomitrella patens

Thermal stress negatively impacts crop yields, and as the overall temperature of the earth’s atmosphere is gradually increasing, the identification of the temperature transduction pathway of the heat signal is essential in developing new strategies in order to adapt plant breeding to warmer climates...

Descripción completa

Detalles Bibliográficos
Autores principales: Elzanati, Osama, Mouzeyar, Said, Roche, Jane
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7073223/
https://www.ncbi.nlm.nih.gov/pubmed/32098429
http://dx.doi.org/10.3390/ijms21041512
_version_ 1783506588588310528
author Elzanati, Osama
Mouzeyar, Said
Roche, Jane
author_facet Elzanati, Osama
Mouzeyar, Said
Roche, Jane
author_sort Elzanati, Osama
collection PubMed
description Thermal stress negatively impacts crop yields, and as the overall temperature of the earth’s atmosphere is gradually increasing, the identification of the temperature transduction pathway of the heat signal is essential in developing new strategies in order to adapt plant breeding to warmer climates. Heat stress damages the molecular structures and physiological processes in plants in proportion to the level and duration of the stress, which leads to different types of responses. In general, plants respond more efficiently when they are first subjected to a moderate temperature increase before being subjected to a higher temperature stress. This adaptive response is called the acclimation period and has been investigated in several plant species. However, there is a lack of information on the dynamic of the Heat Shock Response (HSR) over a continuous period of temperature rise without an acclimation period. In this paper, we investigated the effects of mild (30 °C) and high (37 °C) continuous heat stress over a 24-h period. Through RNA-Seq analysis, we assessed the remodeling of the transcriptome in the moss Physcomitrella patens. Our results showed that the 30 °C treatment particularly affected the expression of a few genes at 1 and 24 h, suggesting a biphasic response. Up-regulated genes at 1 h encode mainly HSR proteins (protein folding and endoplasmic reticulum stress), indicating an early heat response; while the up-regulated genes at 24 h belong to the thiamine biosynthesis pathway. In contrast, the genes involved in photosynthesis and carbon partitioning were repressed by this treatment. Under a higher temperature stress (37 °C), the induction of the HSR occurred rapidly (1 h) and was then attenuated throughout the time points investigated. A network approach (Weighted Gene Correlation Network Analysis, WGCNA) was used to identify the groups of genes expressing similar profiles, highlighting a HsfA1E binding motif within the promoters of some unrelated genes which displayed rapid and transient heat-activation. Therefore, it could be suggested that these genes could be direct targets of activation by a HsfA1E transcription factors.
format Online
Article
Text
id pubmed-7073223
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-70732232020-03-19 Dynamics of the Transcriptome Response to Heat in the Moss, Physcomitrella patens Elzanati, Osama Mouzeyar, Said Roche, Jane Int J Mol Sci Article Thermal stress negatively impacts crop yields, and as the overall temperature of the earth’s atmosphere is gradually increasing, the identification of the temperature transduction pathway of the heat signal is essential in developing new strategies in order to adapt plant breeding to warmer climates. Heat stress damages the molecular structures and physiological processes in plants in proportion to the level and duration of the stress, which leads to different types of responses. In general, plants respond more efficiently when they are first subjected to a moderate temperature increase before being subjected to a higher temperature stress. This adaptive response is called the acclimation period and has been investigated in several plant species. However, there is a lack of information on the dynamic of the Heat Shock Response (HSR) over a continuous period of temperature rise without an acclimation period. In this paper, we investigated the effects of mild (30 °C) and high (37 °C) continuous heat stress over a 24-h period. Through RNA-Seq analysis, we assessed the remodeling of the transcriptome in the moss Physcomitrella patens. Our results showed that the 30 °C treatment particularly affected the expression of a few genes at 1 and 24 h, suggesting a biphasic response. Up-regulated genes at 1 h encode mainly HSR proteins (protein folding and endoplasmic reticulum stress), indicating an early heat response; while the up-regulated genes at 24 h belong to the thiamine biosynthesis pathway. In contrast, the genes involved in photosynthesis and carbon partitioning were repressed by this treatment. Under a higher temperature stress (37 °C), the induction of the HSR occurred rapidly (1 h) and was then attenuated throughout the time points investigated. A network approach (Weighted Gene Correlation Network Analysis, WGCNA) was used to identify the groups of genes expressing similar profiles, highlighting a HsfA1E binding motif within the promoters of some unrelated genes which displayed rapid and transient heat-activation. Therefore, it could be suggested that these genes could be direct targets of activation by a HsfA1E transcription factors. MDPI 2020-02-22 /pmc/articles/PMC7073223/ /pubmed/32098429 http://dx.doi.org/10.3390/ijms21041512 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Elzanati, Osama
Mouzeyar, Said
Roche, Jane
Dynamics of the Transcriptome Response to Heat in the Moss, Physcomitrella patens
title Dynamics of the Transcriptome Response to Heat in the Moss, Physcomitrella patens
title_full Dynamics of the Transcriptome Response to Heat in the Moss, Physcomitrella patens
title_fullStr Dynamics of the Transcriptome Response to Heat in the Moss, Physcomitrella patens
title_full_unstemmed Dynamics of the Transcriptome Response to Heat in the Moss, Physcomitrella patens
title_short Dynamics of the Transcriptome Response to Heat in the Moss, Physcomitrella patens
title_sort dynamics of the transcriptome response to heat in the moss, physcomitrella patens
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7073223/
https://www.ncbi.nlm.nih.gov/pubmed/32098429
http://dx.doi.org/10.3390/ijms21041512
work_keys_str_mv AT elzanatiosama dynamicsofthetranscriptomeresponsetoheatinthemossphyscomitrellapatens
AT mouzeyarsaid dynamicsofthetranscriptomeresponsetoheatinthemossphyscomitrellapatens
AT rochejane dynamicsofthetranscriptomeresponsetoheatinthemossphyscomitrellapatens