Micronucleus Assay: The State of Art, and Future Directions

During almost 40 years of use, the micronucleus assay (MN) has become one of the most popular methods to assess genotoxicity of different chemical and physical factors, including ionizing radiation-induced DNA damage. In this minireview, we focus on the position of MN among the other genotoxicity te...

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Autores principales: Sommer, Sylwester, Buraczewska, Iwona, Kruszewski, Marcin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7073234/
https://www.ncbi.nlm.nih.gov/pubmed/32102335
http://dx.doi.org/10.3390/ijms21041534
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author Sommer, Sylwester
Buraczewska, Iwona
Kruszewski, Marcin
author_facet Sommer, Sylwester
Buraczewska, Iwona
Kruszewski, Marcin
author_sort Sommer, Sylwester
collection PubMed
description During almost 40 years of use, the micronucleus assay (MN) has become one of the most popular methods to assess genotoxicity of different chemical and physical factors, including ionizing radiation-induced DNA damage. In this minireview, we focus on the position of MN among the other genotoxicity tests, its usefulness in different applications and visibility by international organizations, such as International Atomic Energy Agency, Organization for Economic Co-operation and Development and International Organization for Standardization. In addition, the mechanism of micronuclei formation is discussed. Finally, foreseen directions of the MN development are pointed, such as automation, buccal cells MN and chromothripsis phenomenon.
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spelling pubmed-70732342020-03-19 Micronucleus Assay: The State of Art, and Future Directions Sommer, Sylwester Buraczewska, Iwona Kruszewski, Marcin Int J Mol Sci Review During almost 40 years of use, the micronucleus assay (MN) has become one of the most popular methods to assess genotoxicity of different chemical and physical factors, including ionizing radiation-induced DNA damage. In this minireview, we focus on the position of MN among the other genotoxicity tests, its usefulness in different applications and visibility by international organizations, such as International Atomic Energy Agency, Organization for Economic Co-operation and Development and International Organization for Standardization. In addition, the mechanism of micronuclei formation is discussed. Finally, foreseen directions of the MN development are pointed, such as automation, buccal cells MN and chromothripsis phenomenon. MDPI 2020-02-24 /pmc/articles/PMC7073234/ /pubmed/32102335 http://dx.doi.org/10.3390/ijms21041534 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Sommer, Sylwester
Buraczewska, Iwona
Kruszewski, Marcin
Micronucleus Assay: The State of Art, and Future Directions
title Micronucleus Assay: The State of Art, and Future Directions
title_full Micronucleus Assay: The State of Art, and Future Directions
title_fullStr Micronucleus Assay: The State of Art, and Future Directions
title_full_unstemmed Micronucleus Assay: The State of Art, and Future Directions
title_short Micronucleus Assay: The State of Art, and Future Directions
title_sort micronucleus assay: the state of art, and future directions
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7073234/
https://www.ncbi.nlm.nih.gov/pubmed/32102335
http://dx.doi.org/10.3390/ijms21041534
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