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Are the Immune Properties of Mesenchymal Stem Cells from Wharton’s Jelly Maintained during Chondrogenic Differentiation?

Background: Umbilical mesenchymal stem/stromal cells (MSCs), and especially those derived from Wharton’s jelly (WJ), are a promising engineering tool for tissue repair in an allogeneic context. This is due to their differentiation capacity and immunological properties, like their immunomodulatory po...

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Autores principales: Voisin, Charlotte, Cauchois, Ghislaine, Reppel, Loïc, Laroye, Caroline, Louarn, Laetitia, Schenowitz, Chantal, Sonon, Paulin, Poras, Isabelle, Wang, Valentine, D. Carosella, Edgardo, Benkirane-Jessel, Nadia, Moreau, Philippe, Rouas-Freiss, Nathalie, Bensoussan, Danièle, Huselstein, Céline
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7073626/
https://www.ncbi.nlm.nih.gov/pubmed/32033151
http://dx.doi.org/10.3390/jcm9020423
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author Voisin, Charlotte
Cauchois, Ghislaine
Reppel, Loïc
Laroye, Caroline
Louarn, Laetitia
Schenowitz, Chantal
Sonon, Paulin
Poras, Isabelle
Wang, Valentine
D. Carosella, Edgardo
Benkirane-Jessel, Nadia
Moreau, Philippe
Rouas-Freiss, Nathalie
Bensoussan, Danièle
Huselstein, Céline
author_facet Voisin, Charlotte
Cauchois, Ghislaine
Reppel, Loïc
Laroye, Caroline
Louarn, Laetitia
Schenowitz, Chantal
Sonon, Paulin
Poras, Isabelle
Wang, Valentine
D. Carosella, Edgardo
Benkirane-Jessel, Nadia
Moreau, Philippe
Rouas-Freiss, Nathalie
Bensoussan, Danièle
Huselstein, Céline
author_sort Voisin, Charlotte
collection PubMed
description Background: Umbilical mesenchymal stem/stromal cells (MSCs), and especially those derived from Wharton’s jelly (WJ), are a promising engineering tool for tissue repair in an allogeneic context. This is due to their differentiation capacity and immunological properties, like their immunomodulatory potential and paracrine activity. Hence, these cells may be considered an Advanced Therapy Medicinal Product (ATMP). The purpose of this work was to differentiate MSCs from WJ (WJ-MSCs) into chondrocytes using a scaffold and to evaluate, in vitro, the immunomodulatory capacities of WJ-MSCs in an allogeneic and inflammatory context, mimicked by IFN-γ and TNF-α priming during the chondrogenic differentiation. Methods: Scaffolds were made from hydrogel composed by alginate enriched in hyaluronic acid (Alg/HA). Chondrogenic differentiation, immunological function, phenotype expression, but also secreted soluble factors were the different parameters followed during 28 days of culture. Results: During chondrocyte differentiation, even in an allogeneic context, WJ-MSCs remained unable to establish the immunological synapse or to induce T cell alloproliferation. Moreover, interestingly, paracrine activity and functional immunomodulation were maintained during cell differentiation. Conclusion: These results show that WJ-MSCs remained hypoimmunogenic and retained immunomodulatory properties even when they had undergone chondrocyte differentiation.
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spelling pubmed-70736262020-03-19 Are the Immune Properties of Mesenchymal Stem Cells from Wharton’s Jelly Maintained during Chondrogenic Differentiation? Voisin, Charlotte Cauchois, Ghislaine Reppel, Loïc Laroye, Caroline Louarn, Laetitia Schenowitz, Chantal Sonon, Paulin Poras, Isabelle Wang, Valentine D. Carosella, Edgardo Benkirane-Jessel, Nadia Moreau, Philippe Rouas-Freiss, Nathalie Bensoussan, Danièle Huselstein, Céline J Clin Med Article Background: Umbilical mesenchymal stem/stromal cells (MSCs), and especially those derived from Wharton’s jelly (WJ), are a promising engineering tool for tissue repair in an allogeneic context. This is due to their differentiation capacity and immunological properties, like their immunomodulatory potential and paracrine activity. Hence, these cells may be considered an Advanced Therapy Medicinal Product (ATMP). The purpose of this work was to differentiate MSCs from WJ (WJ-MSCs) into chondrocytes using a scaffold and to evaluate, in vitro, the immunomodulatory capacities of WJ-MSCs in an allogeneic and inflammatory context, mimicked by IFN-γ and TNF-α priming during the chondrogenic differentiation. Methods: Scaffolds were made from hydrogel composed by alginate enriched in hyaluronic acid (Alg/HA). Chondrogenic differentiation, immunological function, phenotype expression, but also secreted soluble factors were the different parameters followed during 28 days of culture. Results: During chondrocyte differentiation, even in an allogeneic context, WJ-MSCs remained unable to establish the immunological synapse or to induce T cell alloproliferation. Moreover, interestingly, paracrine activity and functional immunomodulation were maintained during cell differentiation. Conclusion: These results show that WJ-MSCs remained hypoimmunogenic and retained immunomodulatory properties even when they had undergone chondrocyte differentiation. MDPI 2020-02-04 /pmc/articles/PMC7073626/ /pubmed/32033151 http://dx.doi.org/10.3390/jcm9020423 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Voisin, Charlotte
Cauchois, Ghislaine
Reppel, Loïc
Laroye, Caroline
Louarn, Laetitia
Schenowitz, Chantal
Sonon, Paulin
Poras, Isabelle
Wang, Valentine
D. Carosella, Edgardo
Benkirane-Jessel, Nadia
Moreau, Philippe
Rouas-Freiss, Nathalie
Bensoussan, Danièle
Huselstein, Céline
Are the Immune Properties of Mesenchymal Stem Cells from Wharton’s Jelly Maintained during Chondrogenic Differentiation?
title Are the Immune Properties of Mesenchymal Stem Cells from Wharton’s Jelly Maintained during Chondrogenic Differentiation?
title_full Are the Immune Properties of Mesenchymal Stem Cells from Wharton’s Jelly Maintained during Chondrogenic Differentiation?
title_fullStr Are the Immune Properties of Mesenchymal Stem Cells from Wharton’s Jelly Maintained during Chondrogenic Differentiation?
title_full_unstemmed Are the Immune Properties of Mesenchymal Stem Cells from Wharton’s Jelly Maintained during Chondrogenic Differentiation?
title_short Are the Immune Properties of Mesenchymal Stem Cells from Wharton’s Jelly Maintained during Chondrogenic Differentiation?
title_sort are the immune properties of mesenchymal stem cells from wharton’s jelly maintained during chondrogenic differentiation?
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7073626/
https://www.ncbi.nlm.nih.gov/pubmed/32033151
http://dx.doi.org/10.3390/jcm9020423
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