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Application of Fisetin to the Quantitation of Serum Albumin
Fisetin (3,3′,4′,7-tetrahydroxyflavone) is a widely distributed natural flavonol. It interacts with albumin, and thereby generates a fluorescence signal quantitatively. Based on such optical characteristics, we postulated that fisetin was applicable to the quantitation of albumin as an indicator. To...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7073753/ https://www.ncbi.nlm.nih.gov/pubmed/32046075 http://dx.doi.org/10.3390/jcm9020459 |
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author | Park, Jung-Min Do, Van Quan Seo, Yoon-Seok Duong, Men Thi Hoai Ahn, Hee-Chul Huh, Hee Jin Lee, Moo-Yeol |
author_facet | Park, Jung-Min Do, Van Quan Seo, Yoon-Seok Duong, Men Thi Hoai Ahn, Hee-Chul Huh, Hee Jin Lee, Moo-Yeol |
author_sort | Park, Jung-Min |
collection | PubMed |
description | Fisetin (3,3′,4′,7-tetrahydroxyflavone) is a widely distributed natural flavonol. It interacts with albumin, and thereby generates a fluorescence signal quantitatively. Based on such optical characteristics, we postulated that fisetin was applicable to the quantitation of albumin as an indicator. To establish the fisetin-based albumin assay, we examined the optical properties of fisetin and fisetin–albumin complex. The assay conditions were fine-tuned to fit for the actual concentration of serum albumin and to generate an optimal signal with a high signal-to-background ratio. The reaction between fisetin and albumin was linear in a wide range of concentrations. Non-protein serum components did not interfere with the reaction. The reactivity of fisetin was apparently specific for albumin among serum proteins. Both plasma and serum were compatible with the assay. The samples could be stored in a refrigerator or a freezer without the loss of reactivity toward fisetin. The generation and decay rates of the signal were acceptable for manual handling. The recovery of fortified albumin in serum was confirmed and the assay was validated with human sera. Fisetin-based albumin assay is suitable for clinical laboratory testing, considering the simple and short procedure, high specificity and sensitivity, linearity over a wide range of albumin concentrations, and, presumably, potential automatability. |
format | Online Article Text |
id | pubmed-7073753 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-70737532020-03-19 Application of Fisetin to the Quantitation of Serum Albumin Park, Jung-Min Do, Van Quan Seo, Yoon-Seok Duong, Men Thi Hoai Ahn, Hee-Chul Huh, Hee Jin Lee, Moo-Yeol J Clin Med Article Fisetin (3,3′,4′,7-tetrahydroxyflavone) is a widely distributed natural flavonol. It interacts with albumin, and thereby generates a fluorescence signal quantitatively. Based on such optical characteristics, we postulated that fisetin was applicable to the quantitation of albumin as an indicator. To establish the fisetin-based albumin assay, we examined the optical properties of fisetin and fisetin–albumin complex. The assay conditions were fine-tuned to fit for the actual concentration of serum albumin and to generate an optimal signal with a high signal-to-background ratio. The reaction between fisetin and albumin was linear in a wide range of concentrations. Non-protein serum components did not interfere with the reaction. The reactivity of fisetin was apparently specific for albumin among serum proteins. Both plasma and serum were compatible with the assay. The samples could be stored in a refrigerator or a freezer without the loss of reactivity toward fisetin. The generation and decay rates of the signal were acceptable for manual handling. The recovery of fortified albumin in serum was confirmed and the assay was validated with human sera. Fisetin-based albumin assay is suitable for clinical laboratory testing, considering the simple and short procedure, high specificity and sensitivity, linearity over a wide range of albumin concentrations, and, presumably, potential automatability. MDPI 2020-02-07 /pmc/articles/PMC7073753/ /pubmed/32046075 http://dx.doi.org/10.3390/jcm9020459 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Park, Jung-Min Do, Van Quan Seo, Yoon-Seok Duong, Men Thi Hoai Ahn, Hee-Chul Huh, Hee Jin Lee, Moo-Yeol Application of Fisetin to the Quantitation of Serum Albumin |
title | Application of Fisetin to the Quantitation of Serum Albumin |
title_full | Application of Fisetin to the Quantitation of Serum Albumin |
title_fullStr | Application of Fisetin to the Quantitation of Serum Albumin |
title_full_unstemmed | Application of Fisetin to the Quantitation of Serum Albumin |
title_short | Application of Fisetin to the Quantitation of Serum Albumin |
title_sort | application of fisetin to the quantitation of serum albumin |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7073753/ https://www.ncbi.nlm.nih.gov/pubmed/32046075 http://dx.doi.org/10.3390/jcm9020459 |
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