Cargando…

Application of Fisetin to the Quantitation of Serum Albumin

Fisetin (3,3′,4′,7-tetrahydroxyflavone) is a widely distributed natural flavonol. It interacts with albumin, and thereby generates a fluorescence signal quantitatively. Based on such optical characteristics, we postulated that fisetin was applicable to the quantitation of albumin as an indicator. To...

Descripción completa

Detalles Bibliográficos
Autores principales: Park, Jung-Min, Do, Van Quan, Seo, Yoon-Seok, Duong, Men Thi Hoai, Ahn, Hee-Chul, Huh, Hee Jin, Lee, Moo-Yeol
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7073753/
https://www.ncbi.nlm.nih.gov/pubmed/32046075
http://dx.doi.org/10.3390/jcm9020459
_version_ 1783506691103391744
author Park, Jung-Min
Do, Van Quan
Seo, Yoon-Seok
Duong, Men Thi Hoai
Ahn, Hee-Chul
Huh, Hee Jin
Lee, Moo-Yeol
author_facet Park, Jung-Min
Do, Van Quan
Seo, Yoon-Seok
Duong, Men Thi Hoai
Ahn, Hee-Chul
Huh, Hee Jin
Lee, Moo-Yeol
author_sort Park, Jung-Min
collection PubMed
description Fisetin (3,3′,4′,7-tetrahydroxyflavone) is a widely distributed natural flavonol. It interacts with albumin, and thereby generates a fluorescence signal quantitatively. Based on such optical characteristics, we postulated that fisetin was applicable to the quantitation of albumin as an indicator. To establish the fisetin-based albumin assay, we examined the optical properties of fisetin and fisetin–albumin complex. The assay conditions were fine-tuned to fit for the actual concentration of serum albumin and to generate an optimal signal with a high signal-to-background ratio. The reaction between fisetin and albumin was linear in a wide range of concentrations. Non-protein serum components did not interfere with the reaction. The reactivity of fisetin was apparently specific for albumin among serum proteins. Both plasma and serum were compatible with the assay. The samples could be stored in a refrigerator or a freezer without the loss of reactivity toward fisetin. The generation and decay rates of the signal were acceptable for manual handling. The recovery of fortified albumin in serum was confirmed and the assay was validated with human sera. Fisetin-based albumin assay is suitable for clinical laboratory testing, considering the simple and short procedure, high specificity and sensitivity, linearity over a wide range of albumin concentrations, and, presumably, potential automatability.
format Online
Article
Text
id pubmed-7073753
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-70737532020-03-19 Application of Fisetin to the Quantitation of Serum Albumin Park, Jung-Min Do, Van Quan Seo, Yoon-Seok Duong, Men Thi Hoai Ahn, Hee-Chul Huh, Hee Jin Lee, Moo-Yeol J Clin Med Article Fisetin (3,3′,4′,7-tetrahydroxyflavone) is a widely distributed natural flavonol. It interacts with albumin, and thereby generates a fluorescence signal quantitatively. Based on such optical characteristics, we postulated that fisetin was applicable to the quantitation of albumin as an indicator. To establish the fisetin-based albumin assay, we examined the optical properties of fisetin and fisetin–albumin complex. The assay conditions were fine-tuned to fit for the actual concentration of serum albumin and to generate an optimal signal with a high signal-to-background ratio. The reaction between fisetin and albumin was linear in a wide range of concentrations. Non-protein serum components did not interfere with the reaction. The reactivity of fisetin was apparently specific for albumin among serum proteins. Both plasma and serum were compatible with the assay. The samples could be stored in a refrigerator or a freezer without the loss of reactivity toward fisetin. The generation and decay rates of the signal were acceptable for manual handling. The recovery of fortified albumin in serum was confirmed and the assay was validated with human sera. Fisetin-based albumin assay is suitable for clinical laboratory testing, considering the simple and short procedure, high specificity and sensitivity, linearity over a wide range of albumin concentrations, and, presumably, potential automatability. MDPI 2020-02-07 /pmc/articles/PMC7073753/ /pubmed/32046075 http://dx.doi.org/10.3390/jcm9020459 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Park, Jung-Min
Do, Van Quan
Seo, Yoon-Seok
Duong, Men Thi Hoai
Ahn, Hee-Chul
Huh, Hee Jin
Lee, Moo-Yeol
Application of Fisetin to the Quantitation of Serum Albumin
title Application of Fisetin to the Quantitation of Serum Albumin
title_full Application of Fisetin to the Quantitation of Serum Albumin
title_fullStr Application of Fisetin to the Quantitation of Serum Albumin
title_full_unstemmed Application of Fisetin to the Quantitation of Serum Albumin
title_short Application of Fisetin to the Quantitation of Serum Albumin
title_sort application of fisetin to the quantitation of serum albumin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7073753/
https://www.ncbi.nlm.nih.gov/pubmed/32046075
http://dx.doi.org/10.3390/jcm9020459
work_keys_str_mv AT parkjungmin applicationoffisetintothequantitationofserumalbumin
AT dovanquan applicationoffisetintothequantitationofserumalbumin
AT seoyoonseok applicationoffisetintothequantitationofserumalbumin
AT duongmenthihoai applicationoffisetintothequantitationofserumalbumin
AT ahnheechul applicationoffisetintothequantitationofserumalbumin
AT huhheejin applicationoffisetintothequantitationofserumalbumin
AT leemooyeol applicationoffisetintothequantitationofserumalbumin