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Oxidant/Antioxidant Status of Breast Cancer Patients in Pre- and Post-Operative Periods

Background and Objectives: The purpose of this study is to evaluate the level of oxidative stress before and after breast cancer surgery. Materials and Methods: Malondialdehyde (MDA) level was tested using a thiobarbituric acid (TBA) assay based on the release of a color complex due to TBA reaction...

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Autores principales: Didžiapetrienė, Janina, Kazbarienė, Birutė, Tikuišis, Renatas, Dulskas, Audrius, Dabkevičienė, Daiva, Lukosevičienė, Vaida, Kontrimavičiūtė, Eglė, Sužiedėlis, Kęstutis, Ostapenko, Valerijus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7073838/
https://www.ncbi.nlm.nih.gov/pubmed/32054000
http://dx.doi.org/10.3390/medicina56020070
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author Didžiapetrienė, Janina
Kazbarienė, Birutė
Tikuišis, Renatas
Dulskas, Audrius
Dabkevičienė, Daiva
Lukosevičienė, Vaida
Kontrimavičiūtė, Eglė
Sužiedėlis, Kęstutis
Ostapenko, Valerijus
author_facet Didžiapetrienė, Janina
Kazbarienė, Birutė
Tikuišis, Renatas
Dulskas, Audrius
Dabkevičienė, Daiva
Lukosevičienė, Vaida
Kontrimavičiūtė, Eglė
Sužiedėlis, Kęstutis
Ostapenko, Valerijus
author_sort Didžiapetrienė, Janina
collection PubMed
description Background and Objectives: The purpose of this study is to evaluate the level of oxidative stress before and after breast cancer surgery. Materials and Methods: Malondialdehyde (MDA) level was tested using a thiobarbituric acid (TBA) assay based on the release of a color complex due to TBA reaction with MDA. The glutathione S-transferase (GST) activity was evaluated by enzymatic conjugation of reduced glutathione (GSH) with 1-chloro-2,4-dinitrobenzene. The level of total glutathione (reduced GSH and oxidized GSSG) was detected using a recycling system by 5,5-dithiobis(2-nitrobenzoic acid). The levels of the indices were determined in the serum of 52 patients before surgery, two hours and five days after surgery, and in 42 healthy women. Results: In the patients over 50 years old the level of MDA was higher after surgery in comparison with before surgery, and GST activity was lower in comparison with the control. The GSH + GSSG level in both ages groups after surgery was lower than in the control. Significant differences of MDA level were detected in patients with stage III after surgery compared to the control. The level of GSH + GSSG was significantly lower in the patients with I–III stages compared to the control. Conclusion: The most expressed changes demonstrate the significance of MDA as a marker to evaluate oxidative stress in breast cancer patients. The degree of oxidative stress depends on the patient’s age and stage of disease. (1) Malondialdehyde can be used as an oxidative stress marker; (2) A higher stage of the disease and older age correspond to a higher rise of malondialdehyde, suggesting more intensive oxidative stress.
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spelling pubmed-70738382020-03-19 Oxidant/Antioxidant Status of Breast Cancer Patients in Pre- and Post-Operative Periods Didžiapetrienė, Janina Kazbarienė, Birutė Tikuišis, Renatas Dulskas, Audrius Dabkevičienė, Daiva Lukosevičienė, Vaida Kontrimavičiūtė, Eglė Sužiedėlis, Kęstutis Ostapenko, Valerijus Medicina (Kaunas) Article Background and Objectives: The purpose of this study is to evaluate the level of oxidative stress before and after breast cancer surgery. Materials and Methods: Malondialdehyde (MDA) level was tested using a thiobarbituric acid (TBA) assay based on the release of a color complex due to TBA reaction with MDA. The glutathione S-transferase (GST) activity was evaluated by enzymatic conjugation of reduced glutathione (GSH) with 1-chloro-2,4-dinitrobenzene. The level of total glutathione (reduced GSH and oxidized GSSG) was detected using a recycling system by 5,5-dithiobis(2-nitrobenzoic acid). The levels of the indices were determined in the serum of 52 patients before surgery, two hours and five days after surgery, and in 42 healthy women. Results: In the patients over 50 years old the level of MDA was higher after surgery in comparison with before surgery, and GST activity was lower in comparison with the control. The GSH + GSSG level in both ages groups after surgery was lower than in the control. Significant differences of MDA level were detected in patients with stage III after surgery compared to the control. The level of GSH + GSSG was significantly lower in the patients with I–III stages compared to the control. Conclusion: The most expressed changes demonstrate the significance of MDA as a marker to evaluate oxidative stress in breast cancer patients. The degree of oxidative stress depends on the patient’s age and stage of disease. (1) Malondialdehyde can be used as an oxidative stress marker; (2) A higher stage of the disease and older age correspond to a higher rise of malondialdehyde, suggesting more intensive oxidative stress. MDPI 2020-02-11 /pmc/articles/PMC7073838/ /pubmed/32054000 http://dx.doi.org/10.3390/medicina56020070 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Didžiapetrienė, Janina
Kazbarienė, Birutė
Tikuišis, Renatas
Dulskas, Audrius
Dabkevičienė, Daiva
Lukosevičienė, Vaida
Kontrimavičiūtė, Eglė
Sužiedėlis, Kęstutis
Ostapenko, Valerijus
Oxidant/Antioxidant Status of Breast Cancer Patients in Pre- and Post-Operative Periods
title Oxidant/Antioxidant Status of Breast Cancer Patients in Pre- and Post-Operative Periods
title_full Oxidant/Antioxidant Status of Breast Cancer Patients in Pre- and Post-Operative Periods
title_fullStr Oxidant/Antioxidant Status of Breast Cancer Patients in Pre- and Post-Operative Periods
title_full_unstemmed Oxidant/Antioxidant Status of Breast Cancer Patients in Pre- and Post-Operative Periods
title_short Oxidant/Antioxidant Status of Breast Cancer Patients in Pre- and Post-Operative Periods
title_sort oxidant/antioxidant status of breast cancer patients in pre- and post-operative periods
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7073838/
https://www.ncbi.nlm.nih.gov/pubmed/32054000
http://dx.doi.org/10.3390/medicina56020070
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