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Oxidant/Antioxidant Status of Breast Cancer Patients in Pre- and Post-Operative Periods
Background and Objectives: The purpose of this study is to evaluate the level of oxidative stress before and after breast cancer surgery. Materials and Methods: Malondialdehyde (MDA) level was tested using a thiobarbituric acid (TBA) assay based on the release of a color complex due to TBA reaction...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7073838/ https://www.ncbi.nlm.nih.gov/pubmed/32054000 http://dx.doi.org/10.3390/medicina56020070 |
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author | Didžiapetrienė, Janina Kazbarienė, Birutė Tikuišis, Renatas Dulskas, Audrius Dabkevičienė, Daiva Lukosevičienė, Vaida Kontrimavičiūtė, Eglė Sužiedėlis, Kęstutis Ostapenko, Valerijus |
author_facet | Didžiapetrienė, Janina Kazbarienė, Birutė Tikuišis, Renatas Dulskas, Audrius Dabkevičienė, Daiva Lukosevičienė, Vaida Kontrimavičiūtė, Eglė Sužiedėlis, Kęstutis Ostapenko, Valerijus |
author_sort | Didžiapetrienė, Janina |
collection | PubMed |
description | Background and Objectives: The purpose of this study is to evaluate the level of oxidative stress before and after breast cancer surgery. Materials and Methods: Malondialdehyde (MDA) level was tested using a thiobarbituric acid (TBA) assay based on the release of a color complex due to TBA reaction with MDA. The glutathione S-transferase (GST) activity was evaluated by enzymatic conjugation of reduced glutathione (GSH) with 1-chloro-2,4-dinitrobenzene. The level of total glutathione (reduced GSH and oxidized GSSG) was detected using a recycling system by 5,5-dithiobis(2-nitrobenzoic acid). The levels of the indices were determined in the serum of 52 patients before surgery, two hours and five days after surgery, and in 42 healthy women. Results: In the patients over 50 years old the level of MDA was higher after surgery in comparison with before surgery, and GST activity was lower in comparison with the control. The GSH + GSSG level in both ages groups after surgery was lower than in the control. Significant differences of MDA level were detected in patients with stage III after surgery compared to the control. The level of GSH + GSSG was significantly lower in the patients with I–III stages compared to the control. Conclusion: The most expressed changes demonstrate the significance of MDA as a marker to evaluate oxidative stress in breast cancer patients. The degree of oxidative stress depends on the patient’s age and stage of disease. (1) Malondialdehyde can be used as an oxidative stress marker; (2) A higher stage of the disease and older age correspond to a higher rise of malondialdehyde, suggesting more intensive oxidative stress. |
format | Online Article Text |
id | pubmed-7073838 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-70738382020-03-19 Oxidant/Antioxidant Status of Breast Cancer Patients in Pre- and Post-Operative Periods Didžiapetrienė, Janina Kazbarienė, Birutė Tikuišis, Renatas Dulskas, Audrius Dabkevičienė, Daiva Lukosevičienė, Vaida Kontrimavičiūtė, Eglė Sužiedėlis, Kęstutis Ostapenko, Valerijus Medicina (Kaunas) Article Background and Objectives: The purpose of this study is to evaluate the level of oxidative stress before and after breast cancer surgery. Materials and Methods: Malondialdehyde (MDA) level was tested using a thiobarbituric acid (TBA) assay based on the release of a color complex due to TBA reaction with MDA. The glutathione S-transferase (GST) activity was evaluated by enzymatic conjugation of reduced glutathione (GSH) with 1-chloro-2,4-dinitrobenzene. The level of total glutathione (reduced GSH and oxidized GSSG) was detected using a recycling system by 5,5-dithiobis(2-nitrobenzoic acid). The levels of the indices were determined in the serum of 52 patients before surgery, two hours and five days after surgery, and in 42 healthy women. Results: In the patients over 50 years old the level of MDA was higher after surgery in comparison with before surgery, and GST activity was lower in comparison with the control. The GSH + GSSG level in both ages groups after surgery was lower than in the control. Significant differences of MDA level were detected in patients with stage III after surgery compared to the control. The level of GSH + GSSG was significantly lower in the patients with I–III stages compared to the control. Conclusion: The most expressed changes demonstrate the significance of MDA as a marker to evaluate oxidative stress in breast cancer patients. The degree of oxidative stress depends on the patient’s age and stage of disease. (1) Malondialdehyde can be used as an oxidative stress marker; (2) A higher stage of the disease and older age correspond to a higher rise of malondialdehyde, suggesting more intensive oxidative stress. MDPI 2020-02-11 /pmc/articles/PMC7073838/ /pubmed/32054000 http://dx.doi.org/10.3390/medicina56020070 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Didžiapetrienė, Janina Kazbarienė, Birutė Tikuišis, Renatas Dulskas, Audrius Dabkevičienė, Daiva Lukosevičienė, Vaida Kontrimavičiūtė, Eglė Sužiedėlis, Kęstutis Ostapenko, Valerijus Oxidant/Antioxidant Status of Breast Cancer Patients in Pre- and Post-Operative Periods |
title | Oxidant/Antioxidant Status of Breast Cancer Patients in Pre- and Post-Operative Periods |
title_full | Oxidant/Antioxidant Status of Breast Cancer Patients in Pre- and Post-Operative Periods |
title_fullStr | Oxidant/Antioxidant Status of Breast Cancer Patients in Pre- and Post-Operative Periods |
title_full_unstemmed | Oxidant/Antioxidant Status of Breast Cancer Patients in Pre- and Post-Operative Periods |
title_short | Oxidant/Antioxidant Status of Breast Cancer Patients in Pre- and Post-Operative Periods |
title_sort | oxidant/antioxidant status of breast cancer patients in pre- and post-operative periods |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7073838/ https://www.ncbi.nlm.nih.gov/pubmed/32054000 http://dx.doi.org/10.3390/medicina56020070 |
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