Utilizing Stimulated Raman Scattering Microscopy To Study Intracellular Distribution of Label-Free Ponatinib in Live Cells

[Image: see text] Stimulated Raman scattering (SRS) microscopy represents a powerful method for imaging label-free drug distribution with high resolution. SRS was applied to image label-free ponatinib with high sensitivity and specificity in live human chronic myeloid leukemia (CML) cell lines. This...

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Detalles Bibliográficos
Autores principales: Sepp, Kristel, Lee, Martin, Bluntzer, Marie T. J., Helgason, G. Vignir, Hulme, Alison N., Brunton, Valerie G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2019
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7073915/
https://www.ncbi.nlm.nih.gov/pubmed/31829628
http://dx.doi.org/10.1021/acs.jmedchem.9b01546
Descripción
Sumario:[Image: see text] Stimulated Raman scattering (SRS) microscopy represents a powerful method for imaging label-free drug distribution with high resolution. SRS was applied to image label-free ponatinib with high sensitivity and specificity in live human chronic myeloid leukemia (CML) cell lines. This was achieved at biologically relevant, nanomolar concentrations, allowing determination of ponatinib uptake and sequestration into lysosomes during the development of acquired drug resistance and an improved understanding of target engagement.

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