Utilizing Stimulated Raman Scattering Microscopy To Study Intracellular Distribution of Label-Free Ponatinib in Live Cells

[Image: see text] Stimulated Raman scattering (SRS) microscopy represents a powerful method for imaging label-free drug distribution with high resolution. SRS was applied to image label-free ponatinib with high sensitivity and specificity in live human chronic myeloid leukemia (CML) cell lines. This...

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Autores principales: Sepp, Kristel, Lee, Martin, Bluntzer, Marie T. J., Helgason, G. Vignir, Hulme, Alison N., Brunton, Valerie G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2019
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7073915/
https://www.ncbi.nlm.nih.gov/pubmed/31829628
http://dx.doi.org/10.1021/acs.jmedchem.9b01546
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author Sepp, Kristel
Lee, Martin
Bluntzer, Marie T. J.
Helgason, G. Vignir
Hulme, Alison N.
Brunton, Valerie G.
author_facet Sepp, Kristel
Lee, Martin
Bluntzer, Marie T. J.
Helgason, G. Vignir
Hulme, Alison N.
Brunton, Valerie G.
author_sort Sepp, Kristel
collection PubMed
description [Image: see text] Stimulated Raman scattering (SRS) microscopy represents a powerful method for imaging label-free drug distribution with high resolution. SRS was applied to image label-free ponatinib with high sensitivity and specificity in live human chronic myeloid leukemia (CML) cell lines. This was achieved at biologically relevant, nanomolar concentrations, allowing determination of ponatinib uptake and sequestration into lysosomes during the development of acquired drug resistance and an improved understanding of target engagement.
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spelling pubmed-70739152020-03-17 Utilizing Stimulated Raman Scattering Microscopy To Study Intracellular Distribution of Label-Free Ponatinib in Live Cells Sepp, Kristel Lee, Martin Bluntzer, Marie T. J. Helgason, G. Vignir Hulme, Alison N. Brunton, Valerie G. J Med Chem [Image: see text] Stimulated Raman scattering (SRS) microscopy represents a powerful method for imaging label-free drug distribution with high resolution. SRS was applied to image label-free ponatinib with high sensitivity and specificity in live human chronic myeloid leukemia (CML) cell lines. This was achieved at biologically relevant, nanomolar concentrations, allowing determination of ponatinib uptake and sequestration into lysosomes during the development of acquired drug resistance and an improved understanding of target engagement. American Chemical Society 2019-12-12 2020-03-12 /pmc/articles/PMC7073915/ /pubmed/31829628 http://dx.doi.org/10.1021/acs.jmedchem.9b01546 Text en Copyright © 2019 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Sepp, Kristel
Lee, Martin
Bluntzer, Marie T. J.
Helgason, G. Vignir
Hulme, Alison N.
Brunton, Valerie G.
Utilizing Stimulated Raman Scattering Microscopy To Study Intracellular Distribution of Label-Free Ponatinib in Live Cells
title Utilizing Stimulated Raman Scattering Microscopy To Study Intracellular Distribution of Label-Free Ponatinib in Live Cells
title_full Utilizing Stimulated Raman Scattering Microscopy To Study Intracellular Distribution of Label-Free Ponatinib in Live Cells
title_fullStr Utilizing Stimulated Raman Scattering Microscopy To Study Intracellular Distribution of Label-Free Ponatinib in Live Cells
title_full_unstemmed Utilizing Stimulated Raman Scattering Microscopy To Study Intracellular Distribution of Label-Free Ponatinib in Live Cells
title_short Utilizing Stimulated Raman Scattering Microscopy To Study Intracellular Distribution of Label-Free Ponatinib in Live Cells
title_sort utilizing stimulated raman scattering microscopy to study intracellular distribution of label-free ponatinib in live cells
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7073915/
https://www.ncbi.nlm.nih.gov/pubmed/31829628
http://dx.doi.org/10.1021/acs.jmedchem.9b01546
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