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Fluorescent Light Energy (FLE) Acts on Mitochondrial Physiology Improving Wound Healing
Fluorescent light energy (FLE) has been used to treat various injured tissues in a non-pharmacological and non-thermal fashion. It was applied to stimulate cell proliferation, accelerate healing in chronic and acute wounds, and reduce pain and inflammation. FLE has been shown to reduce pro-inflammat...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7073965/ https://www.ncbi.nlm.nih.gov/pubmed/32085605 http://dx.doi.org/10.3390/jcm9020559 |
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author | Ferroni, Letizia Zago, Michela Patergnani, Simone Campbell, Shannon E. Hébert, Lise Nielsen, Michael Scarpa, Carlotta Bassetto, Franco Pinton, Paolo Zavan, Barbara |
author_facet | Ferroni, Letizia Zago, Michela Patergnani, Simone Campbell, Shannon E. Hébert, Lise Nielsen, Michael Scarpa, Carlotta Bassetto, Franco Pinton, Paolo Zavan, Barbara |
author_sort | Ferroni, Letizia |
collection | PubMed |
description | Fluorescent light energy (FLE) has been used to treat various injured tissues in a non-pharmacological and non-thermal fashion. It was applied to stimulate cell proliferation, accelerate healing in chronic and acute wounds, and reduce pain and inflammation. FLE has been shown to reduce pro-inflammatory cytokines while promoting an environment conducive to healing. A possible mechanism of action of FLE is linked to regulation of mitochondrial homeostasis. This work aims to investigate the effect of FLE on mitochondrial homeostasis in an in vitro model of inflammation. Confocal microscopy and gene expression profiling were performed on cultures of inflamed human dermal fibroblasts treated with either direct light from a multi-LED lamp, or FLE from either an amorphous gel or sheet hydrogel matrix. Assessment using confocal microscopy revealed mitochondrial fragmentation in inflamed cells, likely due to exposure to inflammatory cytokines, however, mitochondrial networks were restored to normal 24-h after treatment with FLE. Moreover, gene expression analysis found that treatment with FLE resulted in upregulation of uncoupling protein 1 (UCP1) and carnitine palmitoyltransferase 1B (CPT1B) genes, which encode proteins favoring mitochondrial ATP production through oxidative phosphorylation and lipid β-oxidation, respectively. These observations demonstrate a beneficial effect of FLE on mitochondrial homeostasis in inflamed cells. |
format | Online Article Text |
id | pubmed-7073965 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-70739652020-03-19 Fluorescent Light Energy (FLE) Acts on Mitochondrial Physiology Improving Wound Healing Ferroni, Letizia Zago, Michela Patergnani, Simone Campbell, Shannon E. Hébert, Lise Nielsen, Michael Scarpa, Carlotta Bassetto, Franco Pinton, Paolo Zavan, Barbara J Clin Med Article Fluorescent light energy (FLE) has been used to treat various injured tissues in a non-pharmacological and non-thermal fashion. It was applied to stimulate cell proliferation, accelerate healing in chronic and acute wounds, and reduce pain and inflammation. FLE has been shown to reduce pro-inflammatory cytokines while promoting an environment conducive to healing. A possible mechanism of action of FLE is linked to regulation of mitochondrial homeostasis. This work aims to investigate the effect of FLE on mitochondrial homeostasis in an in vitro model of inflammation. Confocal microscopy and gene expression profiling were performed on cultures of inflamed human dermal fibroblasts treated with either direct light from a multi-LED lamp, or FLE from either an amorphous gel or sheet hydrogel matrix. Assessment using confocal microscopy revealed mitochondrial fragmentation in inflamed cells, likely due to exposure to inflammatory cytokines, however, mitochondrial networks were restored to normal 24-h after treatment with FLE. Moreover, gene expression analysis found that treatment with FLE resulted in upregulation of uncoupling protein 1 (UCP1) and carnitine palmitoyltransferase 1B (CPT1B) genes, which encode proteins favoring mitochondrial ATP production through oxidative phosphorylation and lipid β-oxidation, respectively. These observations demonstrate a beneficial effect of FLE on mitochondrial homeostasis in inflamed cells. MDPI 2020-02-18 /pmc/articles/PMC7073965/ /pubmed/32085605 http://dx.doi.org/10.3390/jcm9020559 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ferroni, Letizia Zago, Michela Patergnani, Simone Campbell, Shannon E. Hébert, Lise Nielsen, Michael Scarpa, Carlotta Bassetto, Franco Pinton, Paolo Zavan, Barbara Fluorescent Light Energy (FLE) Acts on Mitochondrial Physiology Improving Wound Healing |
title | Fluorescent Light Energy (FLE) Acts on Mitochondrial Physiology Improving Wound Healing |
title_full | Fluorescent Light Energy (FLE) Acts on Mitochondrial Physiology Improving Wound Healing |
title_fullStr | Fluorescent Light Energy (FLE) Acts on Mitochondrial Physiology Improving Wound Healing |
title_full_unstemmed | Fluorescent Light Energy (FLE) Acts on Mitochondrial Physiology Improving Wound Healing |
title_short | Fluorescent Light Energy (FLE) Acts on Mitochondrial Physiology Improving Wound Healing |
title_sort | fluorescent light energy (fle) acts on mitochondrial physiology improving wound healing |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7073965/ https://www.ncbi.nlm.nih.gov/pubmed/32085605 http://dx.doi.org/10.3390/jcm9020559 |
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