Cargando…

HPTLC-DESI-HRMS-Based Profiling of Anthraquinones in Complex Mixtures—A Proof-of-Concept Study Using Crude Extracts of Chilean Mushrooms

High-performance thin-layer chromatography (HPTLC) coupled with negative ion desorption electrospray ionization high-resolution mass spectrometry (DESI-HRMS) was used for the analysis of anthraquinones in complex crude extracts of Chilean dermocyboid Cortinarii. For this proof-of-concept study, the...

Descripción completa

Detalles Bibliográficos
Autores principales: Laub, Annegret, Sendatzki, Ann-Katrin, Palfner, Götz, Wessjohann, Ludger A., Schmidt, Jürgen, Arnold, Norbert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074031/
https://www.ncbi.nlm.nih.gov/pubmed/32041163
http://dx.doi.org/10.3390/foods9020156
_version_ 1783506746147340288
author Laub, Annegret
Sendatzki, Ann-Katrin
Palfner, Götz
Wessjohann, Ludger A.
Schmidt, Jürgen
Arnold, Norbert
author_facet Laub, Annegret
Sendatzki, Ann-Katrin
Palfner, Götz
Wessjohann, Ludger A.
Schmidt, Jürgen
Arnold, Norbert
author_sort Laub, Annegret
collection PubMed
description High-performance thin-layer chromatography (HPTLC) coupled with negative ion desorption electrospray ionization high-resolution mass spectrometry (DESI-HRMS) was used for the analysis of anthraquinones in complex crude extracts of Chilean dermocyboid Cortinarii. For this proof-of-concept study, the known anthraquinones emodin, physcion, endocrocin, dermolutein, hypericin, and skyrin were identified by their elemental composition. HRMS also allowed the differentiation of the investigated anthraquinones from accompanying compounds with the same nominal mass in the crude extracts. An investigation of the characteristic fragmentation pattern of skyrin in comparison with a reference compound showed, exemplarily, the feasibility of the method for the determination of these coloring, bioactive and chemotaxonomically important marker compounds. Accordingly, we demonstrate that the coupling of HPTLC with DESI-HRMS represents an advanced and efficient technique for the detection of anthraquinones in complex matrices. This analytical approach may be applied in the field of anthraquinone-containing food and plants such as Rheum spp. (rhubarb), Aloe spp., Morinda spp., Cassia spp. and others. Furthermore, the described method can be suitable for the analysis of anthraquinone-based colorants and dyes, which are used in the food, cosmetic, and pharmaceutical industry.
format Online
Article
Text
id pubmed-7074031
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-70740312020-03-19 HPTLC-DESI-HRMS-Based Profiling of Anthraquinones in Complex Mixtures—A Proof-of-Concept Study Using Crude Extracts of Chilean Mushrooms Laub, Annegret Sendatzki, Ann-Katrin Palfner, Götz Wessjohann, Ludger A. Schmidt, Jürgen Arnold, Norbert Foods Communication High-performance thin-layer chromatography (HPTLC) coupled with negative ion desorption electrospray ionization high-resolution mass spectrometry (DESI-HRMS) was used for the analysis of anthraquinones in complex crude extracts of Chilean dermocyboid Cortinarii. For this proof-of-concept study, the known anthraquinones emodin, physcion, endocrocin, dermolutein, hypericin, and skyrin were identified by their elemental composition. HRMS also allowed the differentiation of the investigated anthraquinones from accompanying compounds with the same nominal mass in the crude extracts. An investigation of the characteristic fragmentation pattern of skyrin in comparison with a reference compound showed, exemplarily, the feasibility of the method for the determination of these coloring, bioactive and chemotaxonomically important marker compounds. Accordingly, we demonstrate that the coupling of HPTLC with DESI-HRMS represents an advanced and efficient technique for the detection of anthraquinones in complex matrices. This analytical approach may be applied in the field of anthraquinone-containing food and plants such as Rheum spp. (rhubarb), Aloe spp., Morinda spp., Cassia spp. and others. Furthermore, the described method can be suitable for the analysis of anthraquinone-based colorants and dyes, which are used in the food, cosmetic, and pharmaceutical industry. MDPI 2020-02-06 /pmc/articles/PMC7074031/ /pubmed/32041163 http://dx.doi.org/10.3390/foods9020156 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Laub, Annegret
Sendatzki, Ann-Katrin
Palfner, Götz
Wessjohann, Ludger A.
Schmidt, Jürgen
Arnold, Norbert
HPTLC-DESI-HRMS-Based Profiling of Anthraquinones in Complex Mixtures—A Proof-of-Concept Study Using Crude Extracts of Chilean Mushrooms
title HPTLC-DESI-HRMS-Based Profiling of Anthraquinones in Complex Mixtures—A Proof-of-Concept Study Using Crude Extracts of Chilean Mushrooms
title_full HPTLC-DESI-HRMS-Based Profiling of Anthraquinones in Complex Mixtures—A Proof-of-Concept Study Using Crude Extracts of Chilean Mushrooms
title_fullStr HPTLC-DESI-HRMS-Based Profiling of Anthraquinones in Complex Mixtures—A Proof-of-Concept Study Using Crude Extracts of Chilean Mushrooms
title_full_unstemmed HPTLC-DESI-HRMS-Based Profiling of Anthraquinones in Complex Mixtures—A Proof-of-Concept Study Using Crude Extracts of Chilean Mushrooms
title_short HPTLC-DESI-HRMS-Based Profiling of Anthraquinones in Complex Mixtures—A Proof-of-Concept Study Using Crude Extracts of Chilean Mushrooms
title_sort hptlc-desi-hrms-based profiling of anthraquinones in complex mixtures—a proof-of-concept study using crude extracts of chilean mushrooms
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074031/
https://www.ncbi.nlm.nih.gov/pubmed/32041163
http://dx.doi.org/10.3390/foods9020156
work_keys_str_mv AT laubannegret hptlcdesihrmsbasedprofilingofanthraquinonesincomplexmixturesaproofofconceptstudyusingcrudeextractsofchileanmushrooms
AT sendatzkiannkatrin hptlcdesihrmsbasedprofilingofanthraquinonesincomplexmixturesaproofofconceptstudyusingcrudeextractsofchileanmushrooms
AT palfnergotz hptlcdesihrmsbasedprofilingofanthraquinonesincomplexmixturesaproofofconceptstudyusingcrudeextractsofchileanmushrooms
AT wessjohannludgera hptlcdesihrmsbasedprofilingofanthraquinonesincomplexmixturesaproofofconceptstudyusingcrudeextractsofchileanmushrooms
AT schmidtjurgen hptlcdesihrmsbasedprofilingofanthraquinonesincomplexmixturesaproofofconceptstudyusingcrudeextractsofchileanmushrooms
AT arnoldnorbert hptlcdesihrmsbasedprofilingofanthraquinonesincomplexmixturesaproofofconceptstudyusingcrudeextractsofchileanmushrooms