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Brassica Rapa SR45a Regulates Drought Tolerance via the Alternative Splicing of Target Genes

The emerging evidence has shown that plant serine/arginine-rich (SR) proteins play a crucial role in abiotic stress responses by regulating the alternative splicing (AS) of key genes. Recently, we have shown that drought stress enhances the expression of SR45a (also known as SR-like 3) in Brassica r...

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Autores principales: Muthusamy, Muthusamy, Yoon, Eun Kyung, Kim, Jin A, Jeong, Mi-Jeong, Lee, Soo In
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074037/
https://www.ncbi.nlm.nih.gov/pubmed/32050656
http://dx.doi.org/10.3390/genes11020182
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author Muthusamy, Muthusamy
Yoon, Eun Kyung
Kim, Jin A
Jeong, Mi-Jeong
Lee, Soo In
author_facet Muthusamy, Muthusamy
Yoon, Eun Kyung
Kim, Jin A
Jeong, Mi-Jeong
Lee, Soo In
author_sort Muthusamy, Muthusamy
collection PubMed
description The emerging evidence has shown that plant serine/arginine-rich (SR) proteins play a crucial role in abiotic stress responses by regulating the alternative splicing (AS) of key genes. Recently, we have shown that drought stress enhances the expression of SR45a (also known as SR-like 3) in Brassica rapa. Herein, we unraveled the hitherto unknown functions of BrSR45a in drought stress response by comparing the phenotypes, chlorophyll a fluorescence and splicing patterns of the drought-responsive genes of Arabidopsis BrSR45a overexpressors (OEs), homozygous mutants (SALK_052345), and controls (Col-0). Overexpression and loss of function did not result in aberrant phenotypes; however, the overexpression of BrSR45a was positively correlated with drought tolerance and the stress recovery rate in an expression-dependent manner. Moreover, OEs showed a higher drought tolerance index during seed germination (38.16%) than the control lines. Additionally, the overexpression of BrSR45a induced the expression of the drought stress-inducible genes RD29A, NCED3, and DREB2A under normal conditions. To further illustrate the molecular linkages between BrSR45a and drought tolerance, we investigated the AS patterns of key drought-tolerance and BrSR45a interacting genes in OEs, mutants, and controls under both normal and drought conditions. The splicing patterns of DCP5, RD29A, GOLS1, AKR, U2AF, and SDR were different between overexpressors and mutants under normal conditions. Furthermore, drought stress altered the splicing patterns of NCED2, SQE, UPF1, U4/U6-U5 tri-snRNP-associated protein, and UPF1 between OEs and mutants, indicating that both overexpression and loss of function differently influenced the splicing patterns of target genes. This study revealed that BrSR45a regulates the drought stress response via the alternative splicing of target genes in a concentration-dependent manner.
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spelling pubmed-70740372020-03-19 Brassica Rapa SR45a Regulates Drought Tolerance via the Alternative Splicing of Target Genes Muthusamy, Muthusamy Yoon, Eun Kyung Kim, Jin A Jeong, Mi-Jeong Lee, Soo In Genes (Basel) Article The emerging evidence has shown that plant serine/arginine-rich (SR) proteins play a crucial role in abiotic stress responses by regulating the alternative splicing (AS) of key genes. Recently, we have shown that drought stress enhances the expression of SR45a (also known as SR-like 3) in Brassica rapa. Herein, we unraveled the hitherto unknown functions of BrSR45a in drought stress response by comparing the phenotypes, chlorophyll a fluorescence and splicing patterns of the drought-responsive genes of Arabidopsis BrSR45a overexpressors (OEs), homozygous mutants (SALK_052345), and controls (Col-0). Overexpression and loss of function did not result in aberrant phenotypes; however, the overexpression of BrSR45a was positively correlated with drought tolerance and the stress recovery rate in an expression-dependent manner. Moreover, OEs showed a higher drought tolerance index during seed germination (38.16%) than the control lines. Additionally, the overexpression of BrSR45a induced the expression of the drought stress-inducible genes RD29A, NCED3, and DREB2A under normal conditions. To further illustrate the molecular linkages between BrSR45a and drought tolerance, we investigated the AS patterns of key drought-tolerance and BrSR45a interacting genes in OEs, mutants, and controls under both normal and drought conditions. The splicing patterns of DCP5, RD29A, GOLS1, AKR, U2AF, and SDR were different between overexpressors and mutants under normal conditions. Furthermore, drought stress altered the splicing patterns of NCED2, SQE, UPF1, U4/U6-U5 tri-snRNP-associated protein, and UPF1 between OEs and mutants, indicating that both overexpression and loss of function differently influenced the splicing patterns of target genes. This study revealed that BrSR45a regulates the drought stress response via the alternative splicing of target genes in a concentration-dependent manner. MDPI 2020-02-10 /pmc/articles/PMC7074037/ /pubmed/32050656 http://dx.doi.org/10.3390/genes11020182 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Muthusamy, Muthusamy
Yoon, Eun Kyung
Kim, Jin A
Jeong, Mi-Jeong
Lee, Soo In
Brassica Rapa SR45a Regulates Drought Tolerance via the Alternative Splicing of Target Genes
title Brassica Rapa SR45a Regulates Drought Tolerance via the Alternative Splicing of Target Genes
title_full Brassica Rapa SR45a Regulates Drought Tolerance via the Alternative Splicing of Target Genes
title_fullStr Brassica Rapa SR45a Regulates Drought Tolerance via the Alternative Splicing of Target Genes
title_full_unstemmed Brassica Rapa SR45a Regulates Drought Tolerance via the Alternative Splicing of Target Genes
title_short Brassica Rapa SR45a Regulates Drought Tolerance via the Alternative Splicing of Target Genes
title_sort brassica rapa sr45a regulates drought tolerance via the alternative splicing of target genes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074037/
https://www.ncbi.nlm.nih.gov/pubmed/32050656
http://dx.doi.org/10.3390/genes11020182
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