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Influence of Acid Adaptation on the Probability of Germination of Clostridium sporogenes Spores Against pH, NaCl and Time
The Clostridium sp. is a large group of spore-forming, facultative or strictly anaerobic, Gram-positive bacteria that can produce food poisoning. The table olive industry is demanding alternative formulations to respond to market demand for the reduction of acidity and salt contents in final product...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074042/ https://www.ncbi.nlm.nih.gov/pubmed/31991695 http://dx.doi.org/10.3390/foods9020127 |
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author | Valero, Antonio Olague, Elena Medina-Pradas, Eduardo Garrido-Fernández, Antonio Romero-Gil, Verónica Cantalejo, María Jesús García-Gimeno, Rosa María Pérez-Rodríguez, Fernando Posada-Izquierdo, Guiomar Denisse Arroyo-López, Francisco Noé |
author_facet | Valero, Antonio Olague, Elena Medina-Pradas, Eduardo Garrido-Fernández, Antonio Romero-Gil, Verónica Cantalejo, María Jesús García-Gimeno, Rosa María Pérez-Rodríguez, Fernando Posada-Izquierdo, Guiomar Denisse Arroyo-López, Francisco Noé |
author_sort | Valero, Antonio |
collection | PubMed |
description | The Clostridium sp. is a large group of spore-forming, facultative or strictly anaerobic, Gram-positive bacteria that can produce food poisoning. The table olive industry is demanding alternative formulations to respond to market demand for the reduction of acidity and salt contents in final products. while maintaining the appearance of freshness of fruits. In this work, logistic regression models for non-adapted and acid-adapted Clostridium sp. strains were developed in laboratory medium to study the influence of pH, NaCl (%) and time on the probability of germination of their spores. A Clostridium sporogenes cocktail was not able to germinate at pH < 5.0, although the adaptation of the strains produced an increase in the probability of germination at 5.0–5.5 pH levels and 6% NaCl concentration. At acidic pH values (5.0), the adapted strains germinated after 10 days of incubation, while those which were non-adapted required 15 days. At pH 5.75 and with 4% NaCl, germination of the adapted strains took place before 7 days, while several replicates of the non-adapted strains did not germinate after 42 days of storage. The model was validated in natural green olive brines with good results (>81.7% correct prediction cases). The information will be useful for the industry and administration to assess the safety risk in the formulation of new processing conditions in table olives and other fermented vegetables. |
format | Online Article Text |
id | pubmed-7074042 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-70740422020-03-19 Influence of Acid Adaptation on the Probability of Germination of Clostridium sporogenes Spores Against pH, NaCl and Time Valero, Antonio Olague, Elena Medina-Pradas, Eduardo Garrido-Fernández, Antonio Romero-Gil, Verónica Cantalejo, María Jesús García-Gimeno, Rosa María Pérez-Rodríguez, Fernando Posada-Izquierdo, Guiomar Denisse Arroyo-López, Francisco Noé Foods Article The Clostridium sp. is a large group of spore-forming, facultative or strictly anaerobic, Gram-positive bacteria that can produce food poisoning. The table olive industry is demanding alternative formulations to respond to market demand for the reduction of acidity and salt contents in final products. while maintaining the appearance of freshness of fruits. In this work, logistic regression models for non-adapted and acid-adapted Clostridium sp. strains were developed in laboratory medium to study the influence of pH, NaCl (%) and time on the probability of germination of their spores. A Clostridium sporogenes cocktail was not able to germinate at pH < 5.0, although the adaptation of the strains produced an increase in the probability of germination at 5.0–5.5 pH levels and 6% NaCl concentration. At acidic pH values (5.0), the adapted strains germinated after 10 days of incubation, while those which were non-adapted required 15 days. At pH 5.75 and with 4% NaCl, germination of the adapted strains took place before 7 days, while several replicates of the non-adapted strains did not germinate after 42 days of storage. The model was validated in natural green olive brines with good results (>81.7% correct prediction cases). The information will be useful for the industry and administration to assess the safety risk in the formulation of new processing conditions in table olives and other fermented vegetables. MDPI 2020-01-24 /pmc/articles/PMC7074042/ /pubmed/31991695 http://dx.doi.org/10.3390/foods9020127 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Valero, Antonio Olague, Elena Medina-Pradas, Eduardo Garrido-Fernández, Antonio Romero-Gil, Verónica Cantalejo, María Jesús García-Gimeno, Rosa María Pérez-Rodríguez, Fernando Posada-Izquierdo, Guiomar Denisse Arroyo-López, Francisco Noé Influence of Acid Adaptation on the Probability of Germination of Clostridium sporogenes Spores Against pH, NaCl and Time |
title | Influence of Acid Adaptation on the Probability of Germination of Clostridium sporogenes Spores Against pH, NaCl and Time |
title_full | Influence of Acid Adaptation on the Probability of Germination of Clostridium sporogenes Spores Against pH, NaCl and Time |
title_fullStr | Influence of Acid Adaptation on the Probability of Germination of Clostridium sporogenes Spores Against pH, NaCl and Time |
title_full_unstemmed | Influence of Acid Adaptation on the Probability of Germination of Clostridium sporogenes Spores Against pH, NaCl and Time |
title_short | Influence of Acid Adaptation on the Probability of Germination of Clostridium sporogenes Spores Against pH, NaCl and Time |
title_sort | influence of acid adaptation on the probability of germination of clostridium sporogenes spores against ph, nacl and time |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074042/ https://www.ncbi.nlm.nih.gov/pubmed/31991695 http://dx.doi.org/10.3390/foods9020127 |
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