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Accuracy of the QIAxcel Automated System for MIRU-VNTR Genotyping of Mycobacterium tuberculosis in Two Limited Resource Settings

Mycobacterial interspersed repetitive units variable number tandem repeat (MIRU-VNTR) typing of Mycobacterium tuberculosis complex (MTBC) isolates, based on 24 loci, is still widely used as the standard for routine molecular surveillance of tuberculosis (TB). QIAxcel system is proposed as an afforda...

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Autores principales: Tafaj, Silva, Ghariani, Asma, Trovato, Alberto, Kapisyzi, Perlat, Essalah, Leila, Mehiri, Emna, Kasmi, Gentian, Burazeri, Genc, Slim Saidi, Leila, Cirillo, Daniela Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074178/
https://www.ncbi.nlm.nih.gov/pubmed/32024091
http://dx.doi.org/10.3390/jcm9020389
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author Tafaj, Silva
Ghariani, Asma
Trovato, Alberto
Kapisyzi, Perlat
Essalah, Leila
Mehiri, Emna
Kasmi, Gentian
Burazeri, Genc
Slim Saidi, Leila
Cirillo, Daniela Maria
author_facet Tafaj, Silva
Ghariani, Asma
Trovato, Alberto
Kapisyzi, Perlat
Essalah, Leila
Mehiri, Emna
Kasmi, Gentian
Burazeri, Genc
Slim Saidi, Leila
Cirillo, Daniela Maria
author_sort Tafaj, Silva
collection PubMed
description Mycobacterial interspersed repetitive units variable number tandem repeat (MIRU-VNTR) typing of Mycobacterium tuberculosis complex (MTBC) isolates, based on 24 loci, is still widely used as the standard for routine molecular surveillance of tuberculosis (TB). QIAxcel system is proposed as an affordable tool that could replace conventional gel electrophoresis and provide high concordance with the reference methods regarding MIRU-VNTR typing of MTBC. We aimed to evaluate the QIAxcel accuracy for allele calling of MIRU-VNTR loci in two regional reference laboratories. A total of 173 DNA were used for the study. Results obtained with QIAxcel were compared to the reference results obtained with an ABI 3730 DNA analyzer. In Albania, the overall agreement with the reference method was 97.92%. A complete agreement result was obtained for 17 loci. In Tunisia, the overall agreement with the reference method was 98.95%. A complete agreement result was obtained for 17 loci. Overall agreement in both centers was 98.43%. In our opinion, use of QIAxcel technology has the potential to be reliable, given an optimized algorithm. Inaccuracies in sizing of long fragments should be solved, especially regarding locus 4052.
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spelling pubmed-70741782020-03-19 Accuracy of the QIAxcel Automated System for MIRU-VNTR Genotyping of Mycobacterium tuberculosis in Two Limited Resource Settings Tafaj, Silva Ghariani, Asma Trovato, Alberto Kapisyzi, Perlat Essalah, Leila Mehiri, Emna Kasmi, Gentian Burazeri, Genc Slim Saidi, Leila Cirillo, Daniela Maria J Clin Med Article Mycobacterial interspersed repetitive units variable number tandem repeat (MIRU-VNTR) typing of Mycobacterium tuberculosis complex (MTBC) isolates, based on 24 loci, is still widely used as the standard for routine molecular surveillance of tuberculosis (TB). QIAxcel system is proposed as an affordable tool that could replace conventional gel electrophoresis and provide high concordance with the reference methods regarding MIRU-VNTR typing of MTBC. We aimed to evaluate the QIAxcel accuracy for allele calling of MIRU-VNTR loci in two regional reference laboratories. A total of 173 DNA were used for the study. Results obtained with QIAxcel were compared to the reference results obtained with an ABI 3730 DNA analyzer. In Albania, the overall agreement with the reference method was 97.92%. A complete agreement result was obtained for 17 loci. In Tunisia, the overall agreement with the reference method was 98.95%. A complete agreement result was obtained for 17 loci. Overall agreement in both centers was 98.43%. In our opinion, use of QIAxcel technology has the potential to be reliable, given an optimized algorithm. Inaccuracies in sizing of long fragments should be solved, especially regarding locus 4052. MDPI 2020-02-01 /pmc/articles/PMC7074178/ /pubmed/32024091 http://dx.doi.org/10.3390/jcm9020389 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tafaj, Silva
Ghariani, Asma
Trovato, Alberto
Kapisyzi, Perlat
Essalah, Leila
Mehiri, Emna
Kasmi, Gentian
Burazeri, Genc
Slim Saidi, Leila
Cirillo, Daniela Maria
Accuracy of the QIAxcel Automated System for MIRU-VNTR Genotyping of Mycobacterium tuberculosis in Two Limited Resource Settings
title Accuracy of the QIAxcel Automated System for MIRU-VNTR Genotyping of Mycobacterium tuberculosis in Two Limited Resource Settings
title_full Accuracy of the QIAxcel Automated System for MIRU-VNTR Genotyping of Mycobacterium tuberculosis in Two Limited Resource Settings
title_fullStr Accuracy of the QIAxcel Automated System for MIRU-VNTR Genotyping of Mycobacterium tuberculosis in Two Limited Resource Settings
title_full_unstemmed Accuracy of the QIAxcel Automated System for MIRU-VNTR Genotyping of Mycobacterium tuberculosis in Two Limited Resource Settings
title_short Accuracy of the QIAxcel Automated System for MIRU-VNTR Genotyping of Mycobacterium tuberculosis in Two Limited Resource Settings
title_sort accuracy of the qiaxcel automated system for miru-vntr genotyping of mycobacterium tuberculosis in two limited resource settings
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7074178/
https://www.ncbi.nlm.nih.gov/pubmed/32024091
http://dx.doi.org/10.3390/jcm9020389
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